长链非编码RNA MUC5B-AS1在人肺腺癌细胞系中的功能研究

王贵露; 袁帅; 胡泽曜; 刘庆云; 向颖; 吴龙; 邬娜; 李成英; 余祖滨; 白莉; 杨敬源; 李亚斐

doi:10.16016/j.1000-5404.201705020

您当前的位置：

首页 >

文章列表页 >

长链非编码RNA MUC5B-AS1在人肺腺癌细胞系中的功能研究

基础医学 | 更新时间：2026-01-09

- 长链非编码RNA MUC5B-AS1在人肺腺癌细胞系中的功能研究
- Functional study of LncRNA MUC5B-AS1 in lung adenocarcinoma cell lines
- 第三军医大学学报 2017年39卷第21期页码：2072-2077
- 作者机构：
  
  1.550000 贵阳，贵州医科大学公共卫生学院流行病学教研室
  2.400038 重庆，第三军医大学军事预防医学院军队流行病学教研室
  3.400037 重庆，第三军医大学新桥医院：胸外科
  4.400037 重庆，第三军医大学新桥医院：全军呼吸内科研究所，全军呼吸病研究重点实验室
- 作者简介：
  
  [李亚斐，电话：(023)68752293, E-mail:liyafei2008@hotmail.comLI Yafei, Tel:86-23-68752293, E-mail:liyafei2008@hotmail.com
- 基金信息：
  
  国家自然科学基金面上项目(81472190);国家自然科学基金面上项目(81672316);国家自然科学基金面上项目(81171903);重庆市自然科学基金重点项目(CSTC2015jcyBX0110)
- DOI：10.16016/j.1000-5404.201705020
  中图分类号： R394.2;R730.23;R734.2
- 收稿：2017-05-04，
  
  修回：2017-07-04，
  
  纸质出版：2017-11-15
- 稿件说明：
移动端阅览
王贵露, 袁帅, 胡泽曜, 等. 长链非编码RNA MUC5B-AS1在人肺腺癌细胞系中的功能研究[J]. 第三军医大学学报, 2017,39(21):2072-2077.

Guilu WANG, Shuai YUAN, Zeyao HU, et al. Functional study of LncRNA MUC5B-AS1 in lung adenocarcinoma cell lines[J]. Journal of Third Military Medical University, 2017, 39(21): 2072-2077.
王贵露, 袁帅, 胡泽曜, 等. 长链非编码RNA MUC5B-AS1在人肺腺癌细胞系中的功能研究[J]. 第三军医大学学报, 2017,39(21):2072-2077. DOI： 10.16016/j.1000-5404.201705020.

Guilu WANG, Shuai YUAN, Zeyao HU, et al. Functional study of LncRNA MUC5B-AS1 in lung adenocarcinoma cell lines[J]. Journal of Third Military Medical University, 2017, 39(21): 2072-2077. DOI： 10.16016/j.1000-5404.201705020.

摘要

目的

研究lncRNA MUC5B-AS1基因在肺腺癌中的表达情况及对肺腺癌细胞系生物学行为的影响。

方法

利用RT-PCR、qRT-PCR检测肺腺癌细胞系中MUC5B-AS1基因的表达情况；构建MUC5B-AS1过表达载体，通过转染构建MUC5B-AS1过表达细胞系；采用CCK-8检测肺腺癌细胞的增殖情况；应用Transwell小室检测MUC5B-AS1过表达对肺腺癌细胞系迁移和侵袭的影响。

结果

检测了MUC5B-AS1在4株肺腺癌细胞系(A549、SPCA1、H1975和H1299)和1株肺正常上皮细胞系(HBE)中的表达情况，发现MUC5B-AS1在H1299细胞系中表达最低，同时A549细胞系表达最高；通过转染构建MUC5B-AS1过表达H1299、A549细胞系，利用qRT-PCR检测细胞系中MUC5B-AS1基因的表达，与对照组比较，MUC5B-AS1基因在H1299、A549细胞系均升高，且有统计学差异(

0.05)；CCK-8检测细胞增殖，与对照组比较过表达MUC5B-AS1对H1299、A549细胞生长并无影响(

0.05)；应用Transwell小室检测MUC5B-AS1过表达对肺腺癌细胞系迁移和侵袭，过表达MUC5B-AS1促进H1299、A549细胞的迁移和侵袭，差异有统计学意义(

0.05)。

结论

MUC5B-AS1可能在肺癌的迁移和侵袭发挥重要作用，但对肺腺癌细胞的增殖能力没有显著影响。

Abstract

Objective

To investigate the expression profiles of lncRNA MUC5B-AS1 in lung adenocarcinoma cell lines and the effect of MUC5B-AS1 on the biological behaviors of the lung adenocarcinoma cells.

Methods

The expression level of MUC5B-AS1 in 4 lung adenocarcinoma cell lines (H1299

H1975

SPCA1 and A549) and a normal lung epithelial cell line (HBE) was detected by reverse transcription PCR (RT-PCR) and real-time quantitative PCR (qRT-PCR). LncRNA MUC5B-AS1 was overexpressed by transfection of overexpression vector in H1299 and A549 cells. Cell counting kit-8 (CCK-8) assays were performed to measure the proliferation of lung adenocarcinoma cell lines. Transwell chamber assay was performed to observe the cell invasion and migration ability in the MUC5B-AS1 overexpression H1299 and A549 cells.

Results

MUC5B-AS1 was expressed in 4 strains of lung adenocarcinoma cell lines and normal lung epithelial cell line. Its expression level was quite lower in the H1299 cells

but higher in the A549 cells than other cell lines. After transfection of overexpression vector in the H1299 and A549 cells

the expression of MUC5B-AS1 was remarkably enhanced (

0.05). CCK-8 assays indicated that MUC5B-AS1 overexpression had no effect on the proliferation in H1299 and A549 cells when compared with non-transfected cells (

0.05). Transwell chamber assay showed the MUC5B-AS1 overexpression promoted the migration and invasion in the H1299 and A549 cells (

0.05).

Conclusion

MUC5B-AS1 may play an important roles in the migration and invasion in lung adenocarcinoma cells

but had no obvious effect on proliferation.

关键词

Keywords

references

SIEGEL R L, MILLER K D, FEDEWA S A, et al. Colorectal cancer statistics, 2017[J]. CA Cancer J Clin, 2017, 67(3): 177-193. DOI: 10.3322/caac.21395.

PONTING C P, OLIVER P L, REIK W. Evolution and functions of long noncoding RNAs[J]. Cell, 2009, 136(4): 629-641. DOI: 10.1016/j.cell.2009.02.006.

FATICA A, BOZZONI I. Long non-coding RNAs: new players in cell differentiation and development[J]. Nat Rev Genet, 2014, 15(1): 7-21. DOI: 10.1038/nrg3606.

XIE W, YUAN S, SUN Z, et al. Long noncoding and circular RNAs in lung cancer: advances and perspectives[J]. Epigenomics, 2016, 8(9): 1275-1287. DOI: 10.2217/epi-2016-0036.

傅莉媛, 熊刚, 李娟, 等. MTF-1对食管鳞癌EC109细胞株生长和迁移的影响[J].第三军医大学学报, 2014, 36(8): 750-754.DOI:10.16016/j.1000-5404.2014.08.027.

FU L Y, XIONG G, LI J, et al.Effects of MTF-1 on cell prolife-ration and migration in human esophageal carcinoma EC109 cells[J].J Third Mil Med Univ, 2014, 36(8): 750-754. DOI: 10.16016/j.1000-5404.2014.08.027.

OKAZAKI Y, FURUNO M, KASUKAWA T, et al. Analysis of the mouse transcriptome based on functional annotation of 60, 770 full-length cDNAs[J]. Nature, 2002, 420(6915): 563-573. DOI: 10.1038/nature01266.

FATICA A, BOZZONI I. Long non-coding RNAs: new players in cell differentiation and development[J]. Nat Rev Genet, 2014, 15(1): 7-21. DOI: 10.1038/nrg3606.

MATOUK I J, HALLE D, GILON M, et al. The non-coding RNAs of the H19-IGF2 imprinted loci: a focus on biological roles and therapeutic potential in Lung Cancer[J]. J Transl Med, 2015,13: 113. DOI: 10.1186/s12967-015-0467-3.

ONO H, MOTOI N, NAGANO H, et al. Long noncoding RNA HOTAIR is relevant to cellular proliferation, invasiveness, and clinical relapse in s mall-cell lung cancer[J ] . Cancer Med, 2014, 3(3): 632-642. DOI: 10.1002/cam4.220.

GUTSCHNER T, HAMMERLE M, EISSMANN M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells[J]. Cancer Res, 2013, 73(3): 1180-1189. DOI: 10.1158/0008-5472.CAN-12-2850.

LIN L, GU Z T, CHEN W H, et al. Increased expression of the long non-coding RNA ANRIL promotes lung cancer cell metastasis and correlates with poor prognosis.[J]. Diagn Pathol, 2015, 10(1): 14. DOI: 10.1186/s13000-015-0247-7.

DONG S, QU X, LI W, et al. The long non-coding RNA, GAS5, enhances gefitinib-induced cell death in innate EGFR tyrosine kinase inhibitor-resistant lung adenocarcinoma cells with wide-type EGFR via downregulation of the IGF-1R expression[J ] . J Hematol Oncol, 2015, 8: 43. DOI: 10.1186/ s13045-015-0140-6.

LIVRAGHI-BUTRICO A, GRUBB B R, WILKINSON K J, et al. Contribution of mucus concentration and secreted mucins Muc5ac and Muc5b to the pathogenesis of muco-obstructive lung disease[J]. Mucosal Immunology, 2016, 10(2): 395-407. DOI: 10.1038/mi.2016.63.

VALQUE H, GOUYER V, GOTTRAND F, et al. MUC5B leads to aggressive behavior of breast cancer MCF7 cells[J]. PLoS ONE, 2012, 7(10): e46699. DOI: 10.1371/journal.pone. 0046699.

WAKATA K, TSUCHIYA T, TOMOSHIGE K, et al. A favourable prognostic marker for EGFR mutant non-small cell lung cancer: immunohistochemical analysis of MUC5B[J]. BMJ Open, 2015, 5(7): e008366. DOI: 10.1136/ bmjopen-2015-008366.

WU Y, LIU H, SHI X, et al. The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma[J]. Oncotarget, 2015, 6(11): 9160-9172. DOI: 10.18632/oncotarget.3247.

FENG J, SUN Y, ZHANG E B, et al. A novel long noncoding RNA IRAIN regulates cell proliferation in non small cell lung cancer[J]. Int J Clin Exp Pathol, 2015, 8(10): 12268-12275.

MIAO L, HUANG Z, ZENGLI Z, et al. Loss of long noncoding RNA FOXF1-AS1 regulates epithelial-mesenchymal transition, stemness and metastasis of non-small cell lung cancer cells[J]. Oncotarget, 2016, 7(42): 68339-68349. DOI: 10.18632/oncotarget.11630.

JIA X, WANG Z, QIU L, et al. Upregulation of LncRNA-HIT promotes migration and invasion of non-small cell lung cancer cells by association with ZEB1[J]. Cancer Med, 2016, 5(12): 3555-3563. DOI: 10.1002/cam4.948.

BAFNA S, KAUR S, BATRA S K. Membrane-bound mucins: the mechanistic basis for alterations in the growth and survival of cancer cells[J]. Oncogene, 2010, 29(20): 2893-2904. DOI: 10.1038/onc.2010.87.

LEE J, LEE J, YUN J H, et al. DUSP28 links regulation of Mucin 5B and Mucin 16 to migration and survival of AsPC-1 human pancreatic cancer cells[J]. Tumour Biol, 2016, 37(9): 12193-12202. DOI: 10.1007/s13277-016-5079-x.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

长链非编码RNA OSER1-AS1在非小细胞肺癌细胞中的功能研究

RNA结合蛋白TTP对肺腺癌细胞增殖影响的实验研究

GPC5基因在肺腺癌细胞系中的功能研究

超低浓度二甲基亚砜促进皮肤成纤维细胞增殖作用及机制研究

谷胱甘肽过氧化物酶7通过PI3K-AKT通路调控肝癌细胞增殖和衰老