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豚鼠胰腺内telocytes形态学特点及甲磺酸伊马替尼对telocytes的干预作用
Morphological characteristics of telocytes in the pancreas of guinea pigs and effect of imatinib mesylate on the cells
- 第三军医大学学报 2017年39卷第20期 页码：1986-1992
- 作者机构：
550025 贵阳, 贵州医科大学基础医学院人体组织学与胚胎学教研室
- 作者简介：
[黄悦, E-mail: huranggaigaiyu@sina.com HUANG Yue, E-mail:huranggaigaiyu@sina.com
- 基金信息：贵州省科技厅基金([2008]2277);贵阳市科技局贵州医科大学联合基金([20161001]013)
DOI：10.16016/j.1000-5404.201704093
中图分类号： R322.491;R329.24;R977.3收稿：2017-04-18，
修回：2017-05-15，
纸质出版：2017-10-30
- 稿件说明：
移动端阅览
闫丽丽, 严会文, 彭丽娟, 等. 豚鼠胰腺内telocytes形态学特点及甲磺酸伊马替尼对telocytes的干预作用[J]. 第三军医大学学报, 2017,39(20):1986-1992.
Lili YAN, Huiwen YAN, Lijuan PENG, et al. Morphological characteristics of telocytes in the pancreas of guinea pigs and effect of imatinib mesylate on the cells[J]. Journal of Third Military Medical University, 2017, 39(20): 1986-1992.
闫丽丽, 严会文, 彭丽娟, 等. 豚鼠胰腺内telocytes形态学特点及甲磺酸伊马替尼对telocytes的干预作用[J]. 第三军医大学学报, 2017,39(20):1986-1992. DOI： 10.16016/j.1000-5404.201704093.
Lili YAN, Huiwen YAN, Lijuan PENG, et al. Morphological characteristics of telocytes in the pancreas of guinea pigs and effect of imatinib mesylate on the cells[J]. Journal of Third Military Medical University, 2017, 39(20): 1986-1992. DOI： 10.16016/j.1000-5404.201704093.
摘要
目的
2
对豚鼠胰腺组织特络细胞(telocytes，TCs)的形态特征进行观察与分析，并探讨甲磺酸伊马替尼对TCs的干预作用。
方法
2
以健康9周龄豚鼠胰腺为研究对象，利用免疫组化染色对TCs免疫标志(c-kit/CD117、vimentin)进行观察分析。透射电镜观察TCs的超微结构。建立甲磺酸伊马替尼干预模型：豚鼠54只，雌雄不限，分正常对照组、用药组(7、14、21、28 d)、停药组(W7、W14、W21、W28 d)；免疫组化染色观察用/停药后TCs变化；Western blot观察用/停药后胰腺组织内CD117、vimentin蛋白表达。
结果
2
免疫组化染色显示：胰腺内TCs位于结缔组织内，细胞呈现三角形及长梭形等多种形态，有一至多条长而细的细胞突起，突起包绕腺泡，并相连形成网络结构；电镜超微结构显示：TCs有念珠状的细长突起，核质比较高，胞质内含有少量的线粒体、滑面内质网等；甲磺酸伊马替尼干预后，TCs突起变短，网络结构减少，停药后逐渐恢复；用药后胰腺组织内c-kit/CD117和vimentin蛋白表达降低，停药后逐渐恢复，差异有统计学意义(
P
<
0.05)。
结论
2
豚鼠胰腺内存在梭形有念珠状突起的TCs，TCs包绕腺泡形成网络结构；甲磺酸伊马替尼可影响胰腺内TCs的形态及蛋白表达。
Abstract
Objective
2
To observe the morphological characteristics of telocytes(TCs) in the pancreas of guinea pigs
and determine the effect of imatinib mesylate on TCs.
Methods
2
Immunohistochemical staining was used to observe the immune markers
c-kit
CD117 and vimentin in the TCs from the pancreas of guinea pigs(9 weeks old). Transmission electron microscopy(TEM) was used to observed the ultrastructure of TCs. Fifty-four guinea pigs of either sex were randomly divided into normal control group
imatinib mesylate treatment groups (7
14
21 and 28 d) and drug withdrawal groups (in 7
14
21 and 28 d after withdraw). After treatment and drug withdrawal
immunohistochemical staining was employed to observe the changes in the TCs
and Western blotting was adopted to detect the expression changes of c-kit
CD117 and vimentin.
Results
2
Immunohistochemical staining showed that pancreatic TCs were located in the connective tissues
and the cells were in triangular
long spindle and other shapes with one or more long and fine cell processes
protruding around the acini and connected into network structures. ETM results displayed that the TCs had slender moniliform processes
higher nucleus/cytoplasm ratio
and mitochondria and smooth endoplasmic reticula in the cytoplasm. Imatinib mesylate treatment induced shorter cell processes and less network structures
but the phenomena were recovered after drug withdrawal. The drug intervention also decreased the protein levels of c-kit/CD117 and vimentin
and the levels were also restored after the withdrawal (
P
<
0.05).
Conclusion
2
In the pancreas of guinea pigs
there are spindle-shaped TCs with moniliform processes
and the TCs protrude around the acini and connect into network structures. Imatinib mesylate affects the morphology and protein expression in the TCs.
关键词
Keywords
references
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