石榴皮多酚对大鼠应激性胃溃疡的保护作用及与Gli1-Gli2-Sufu信号通路和细胞因子水平改变的关系

赖舒; 张玉方

doi:10.16016/j.1000-5404.201704083

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石榴皮多酚对大鼠应激性胃溃疡的保护作用及与Gli1-Gli2-Sufu信号通路和细胞因子水平改变的关系

基础医学 | 更新时间：2026-01-09

- 石榴皮多酚对大鼠应激性胃溃疡的保护作用及与Gli1-Gli2-Sufu信号通路和细胞因子水平改变的关系
- Protective effects of pomegranate peel polyphenols against stress gastric ulcer in rats and their effect on cytokine levels and Gli1-Gli2-Sufu signaling pathway
- 第三军医大学学报 2017年39卷第19期页码：1913-1918
- 作者机构：
  
  1.400050 重庆，重庆市九龙坡区第一人民医院药剂科
  2.400020 重庆，重庆市江北区人民医院药剂科
- 作者简介：
  
  [ 张玉方，电话：(023)88519015，E-mail：zhangyufang001@163.com ZHANG Yufang, Tel: 86-23-88519015, E-mail:zhangyufang001@163.com
- 基金信息：
  
  重庆市医学科研计划(2013-2-195)
- DOI：10.16016/j.1000-5404.201704083
  中图分类号： R573.1;R914.4;R966
- 收稿：2017-04-17，
  
  修回：2017-06-21，
  
  纸质出版：2017-10-15
- 稿件说明：
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赖舒, 张玉方. 石榴皮多酚对大鼠应激性胃溃疡的保护作用及与Gli1-Gli2-Sufu信号通路和细胞因子水平改变的关系[J]. 第三军医大学学报, 2017,39(19):1913-1918.

Shu LAI, Yufang ZHANG. Protective effects of pomegranate peel polyphenols against stress gastric ulcer in rats and their effect on cytokine levels and Gli1-Gli2-Sufu signaling pathway[J]. Journal of Third Military Medical University, 2017, 39(19): 1913-1918.
赖舒, 张玉方. 石榴皮多酚对大鼠应激性胃溃疡的保护作用及与Gli1-Gli2-Sufu信号通路和细胞因子水平改变的关系[J]. 第三军医大学学报, 2017,39(19):1913-1918. DOI： 10.16016/j.1000-5404.201704083.

Shu LAI, Yufang ZHANG. Protective effects of pomegranate peel polyphenols against stress gastric ulcer in rats and their effect on cytokine levels and Gli1-Gli2-Sufu signaling pathway[J]. Journal of Third Military Medical University, 2017, 39(19): 1913-1918. DOI： 10.16016/j.1000-5404.201704083.

摘要

目的

探讨石榴皮多酚对大鼠应激性胃溃疡的保护作用及与Gli1-Gli2-Sufu信号通路和细胞因子水平改变的关系。

方法

选取50只7周龄健康SD雄性大鼠为受试对象，按随机数字表法分为空白对照组、应激性胃溃疡组(模型组)、奥美拉唑+应激性胃溃疡模型组(阳性对照组)、石榴皮多酚组和应激性胃溃疡模型+石榴皮多酚组，每组10只。分别比较各组大鼠胃液pH值和黏膜损伤指数；采用ELISA法测定前列腺素E2(PGE2)、促肾上腺皮质激素(ATCH)和皮质酮(CORT)的含量，应用化学比色法检测丙二醛(MDA)和超氧化物歧化酶(SOD)；采用qPCR和Western blot法测定各组大鼠胃组织Gli1、Gli2和Sufu的mRNA和蛋白表达水平；免疫共沉淀法观察大鼠胃组织Gli2和Sufu蛋白复合物的形成情况。

结果

与空白对照组比较，模型组大鼠的损伤指数、MDA、ACTH和CORT的含量明显升高，pH值、SOD和PGE2水平显著下降，差异均有统计学意义(

0.05)；但阳性对照组和石榴皮多酚+模型组的上述各项指标均较模型组明显改善(

0.05)；模型组大鼠胃组织的Gli1、Gli2和Sufu的mRNA和蛋白表达均较空白对照组明显升高(

0.05)；而阳性对照组和石榴皮多酚+模型组的Gli1、Gli2和Sufu的mRNA和蛋白表达水平较模型组却明显降低(

0.05)；模型组大鼠Gli2和Sufu蛋白复合物水平较空白对照组显著增多，而阳性对照组和石榴皮多酚+模型组的Gli2和Sufu蛋白复合物水平则较模型组明显降低(

0.05)。

结论

石榴皮多酚对大鼠应激性胃溃疡的保护作用可能与Gli1-Gli2-Sufu信号通路活化和MDA、ACTH、CORT、SOD和PGE2等细胞因子水平改变有关。

Abstract

Objective

To investigate the protective effects of pomegranate peel polyphenols against stress gastric ulcer in rats and its relationship with cytokine levels and Gli2-Sufu signaling pathway.

Methods

A total of 50 male SD rats (7 weeks old) were randomly divided into blank control group

stress ulcer group (model group)

omeprazole-treated gastric ulcer model group (positive control group)

pomegranate peel polyphenol group

and stress ulcer model treated with pomegranate peel polyphenol group

10 rats in each group. The pH value of gastric juice and mucosal damage index were compared in the groups. Serum levels of prostaglandin E2 (PGE2)

adrenocorticotropic hormone (ATCH) and corticosterone (CORT) were detected by ELISA. Malondialdehyde (MDA) and superoxide dismutase (SOD) were assessed by chemical colorimetry. The mRNA and protein expression levels of Gli1

Gli2 and Sufu in the gastric tissue of rats were determined by qPCR and Western blotting. The formations of Gli2 and Sufu protein complexes in the gastric tissue were evaluated by co-immunoprecipitation assay.

Results

Compared with the blank control

the mucosal damage index and contents of MDA

ACTH and CORT were significantly increased

while the pH value and levels of SOD and PGE2 were significantly decreased in the model group (

0.05). However

both omeprazole and pomegranate peel polyphenol treatment significantly improved the above indexes as compared with the model group (

0.05). The mRNA and protein expression levels of Gli1

Gli2 and Sufu were significantly higher in the model group than the blank control group (

0.05)

but the levels were significantly lower in the positive control group and pomegranate peel polyphenol group than in the model group (

0.05). Moreover

the levels of Gli2 and Sufu complexes were significantly higher in the model group than the blank control group

while the levels were significantly lower in the omeprazole and pomegranate peel polyphenol treatment groups than the model group (

0.05).

Conclusion

Pomegranate peel polyphenols exert protective effect on stress gastric ulcer in rats

which may be related to the activation of Gli1-Gli2-Sufu signaling pathway and changes of cytokines

such as MDA

ACTH

CORT

SOD and PGE2.

关键词

Keywords

references

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