IGF-FOXO3a通路对肝癌干细胞自我更新能力的调控

李志芬; 沈俊杰; 单娟娟; 陈军; 钱程

doi:10.16016/j.1000-5404.201703093

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IGF-FOXO3a通路对肝癌干细胞自我更新能力的调控

基础医学 | 更新时间：2026-01-09

- IGF-FOXO3a通路对肝癌干细胞自我更新能力的调控
- IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro
- 第三军医大学学报 2017年39卷第16期页码：1631-1636
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81472292)
- DOI：10.16016/j.1000-5404.201703093
  中图分类号：
- 收稿：2016-03-15，
  
  纸质出版：2017-08-30
- 稿件说明：
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李志芬, 沈俊杰, 单娟娟, 等. IGF-FOXO3a通路对肝癌干细胞自我更新能力的调控[J]. 第三军医大学学报, 2017,39(16):1631-1636.

Zhifen LI, Junjie SHEN, Juanjuan SHAN, et al. IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro[J]. Journal of Third Military Medical University, 2017, 39(16): 1631-1636.
李志芬, 沈俊杰, 单娟娟, 等. IGF-FOXO3a通路对肝癌干细胞自我更新能力的调控[J]. 第三军医大学学报, 2017,39(16):1631-1636. DOI： 10.16016/j.1000-5404.201703093.

Zhifen LI, Junjie SHEN, Juanjuan SHAN, et al. IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro[J]. Journal of Third Military Medical University, 2017, 39(16): 1631-1636. DOI： 10.16016/j.1000-5404.201703093.

摘要

Abstract

Objective

To investigate the role of FOXO3a in the regulation of self-renewal capacity in liver cancer stem cells (LCSCs) and study the related mechanisms.

Methods

Flow cytometry was used to obtain LCSCs

and then the expression of FOXO3a in the attained cells was detected by real time fluorescent quantitative PCR (qRT-PCR). After the activity of insulin-like growth factor(IGF) was changed with IGF inhibitor or activator

the expression of FOXO3a was measured again with Western blotting and qRT-PCR. The lentiviral vectors expressing or interfering FOXO3a were used to infect the cells

and Western blot analysis was used to detect the efficiencies of overexpression and interference. Cell colony formation assay and sphere formation assay were used to test the self-renewal in the cells infected with the lentivirus that expressing or interfering FOXO3a

and the cells treated with IGF activators meanwhile over-expressing FOXO3a.

Results

The expression of FOXO3a was significantly lower in the LCSCs than the liver cancer cells (

0.01)

and it was negatively regulated by IGF. The cell models of stable overexpression or interference of FOXO3a were established successfully. And FOXO3a over-expression resulted in decreases in the colony formation and sphere formation

while FOXO3a interference led to improvement in the formations. Activation of IGF signal

combined with FOXO3a overexpression also induced decreased formations.

Conclusion

FOXO3a exerts a negative effect on the self-renewal in LCSCs

and can be negatively regulated by IGF signaling. It mediates the regulation of IGF signaling on the self-renewal in LCSCs.

关键词

Keywords

references

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