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适度运动对人体肠道菌群结构的影响
Moderate exercise induces shift in the composition of human gut microbiota
- 第三军医大学学报 2017年39卷第18期 页码：1824-1831
- 作者机构：
1.400037 重庆，第三军医大学新桥医院检验科
2.400038 重庆，第三军医大学微生物教研室
- 作者简介：
[ 蒲晓允，E-mail：xqyyjyk@foxmail.com PU Xiaoyun, E-mail:xqyyjyk@foxmail.com
- 基金信息：国家自然科学基金面上项目(31571352)
DOI：10.16016/j.1000-5404.201703049
中图分类号： R161.1; R333.3; R339.4收稿：2017-03-07，
修回：2017-05-01，
纸质出版：2017-09-30
- 稿件说明：
移动端阅览
蒋兴宇, 赵霞, 邹凌云, 等. 适度运动对人体肠道菌群结构的影响[J]. 第三军医大学学报, 2017,39(18):1824-1831.
Xingyu JIANG, Xia ZHAO, Lingyun ZOU, et al. Moderate exercise induces shift in the composition of human gut microbiota[J]. Journal of Third Military Medical University, 2017, 39(18): 1824-1831.
蒋兴宇, 赵霞, 邹凌云, 等. 适度运动对人体肠道菌群结构的影响[J]. 第三军医大学学报, 2017,39(18):1824-1831. DOI： 10.16016/j.1000-5404.201703049.
Xingyu JIANG, Xia ZHAO, Lingyun ZOU, et al. Moderate exercise induces shift in the composition of human gut microbiota[J]. Journal of Third Military Medical University, 2017, 39(18): 1824-1831. DOI： 10.16016/j.1000-5404.201703049.
摘要
目的
2
适度运动有益于身心健康。本研究从肠道菌群结构的角度来探讨运动对人体健康的影响。
方法
2
招募运动组和少动组志愿者人群，利用16S rDNA高通量测序技术分析志愿者肠道菌群结构。
结果
2
高通量测序共获得61 888个OTUs，其中已注释的OTUs有61 149个。PCoA分析显示，运动组和少动组之间肠道菌群结构存在显著差异。运动组中毛螺菌科（
Lachnospiraceae
）和拟杆菌科（
Bacteroidaceae
）的丰度显著高于少动组（
P
=2.5627×10
-7
，
P
=0.000 178 4），普雷沃氏菌科（
Prevotellaceae
）和韦荣氏球菌科（
Veillonellaceae
）的丰度显著低于少动组（
P
=0.000 003 46，
P
=0.000 017 41）。有3株可培养的菌株在运动组中丰度显著增加，分别为瘤胃球菌属的Ruminococcus_sp.5_1_39BFAA，双歧杆菌属的
Bifidobacterium
_
adolescentis
以及丁酸产盐菌属的
Anaerostipes
_
hadrus
。在运动组中显著增加的肠道菌，如拟杆菌科，双歧杆菌属的
Bifidobacterium
_
adolescentis
以及
Anaerostipes
_
hadrus
，能够代谢产生丰富的SCFAs，对肠粘膜免疫系统动态平衡的维持具有重要意义。
结论
2
运动可能通过肠道菌群结构的改变及其与肠黏膜系统的相互作用对人体产生积极影响。
Abstract
Objective
2
To investigate effects of moderate exercise on human health from the perspective of gut microbiota.
Methods
2
Totally 40 volunteers were recruited
and assigned into the exercise group (
n
=20) and the sedentary group (
n
=20) according their life styles. The feces were collected and total fecal DNA was extracted
and then all the microorganisms in the intestinal tract of volunteers were detected and analyzed by using the 16S rDNA-based high throughput sequencing for operational taxonomic units (OTUs) clustering. Species classification analysis was performed according to the sequencing data in order to obtain the corresponding species information and abundance distribution.
Results
2
A total of 61 888 OTUs were obtained from the high throughput sequencing data through OTUs analysis
of which 61 149 OTUs were annotated. The principal co-ordinates analysis (PCoA) indicated that significant differences were observed between the 2 groups. The abundances of
Lachnospiraceae
and
Bacteroidaceae
were significantly higher (
P
=2.5627×10
-7
P
=0.000 178 4)
while those of Prevotellaceae and Veillonellaceae were obviously lower in the moderate exercise group than the sedentary group (
P
=0.000 003 46
P
=0.000 017 41). The abundances of 3 culturable strains (
Ruminococcus
_
sp
.5_1_39
BFAA
Bifidobacterium
_
adolescentis
and butyrate salt producing bacteria
Anaerostipes
_
hadrus
) were significantly increased in the exercise group. The obviously increased bacteria
including Bacteroides
Bifidobacterium
_
adolescentis
of
Bifidobacterium
and
Anaerostipes
_
hadrus
could produce lots of short-chain fatty acids (SCFA)
which were important for the maintenance of dynamic balance of intestinal mucosal immune system.
Conclusion
2
Exercise may have a positive effect on the human body through changes in intestinal flora composition and its interaction with the intestinal mucosal system.
关键词
Keywords
references
WARREN T Y, BARRY V, HOOKER S P, et al. Sedentary behaviors increase risk of cardiovascular disease mortality in men[J]. Med Sci Sports Exerc, 2010, 42(5): 879-885. DOI: 10.1249/MSS.0b013e3181c3aa7e.
OWEN N, HEALY G N, MATTHEWS C E, et al. Too Much Sitting[J]. Exercise and Sport Sciences Reviews, 2010, 38(3): 105-113. DOI: 10.1097/jes.0b013e3181e373a2.
WARBURTON D E, NICOL C W, BREDIN S S. Health benefits of physical activity: the evidence[J]. journal de l'Association medicale canadienne, 2006, 174(6): 801-809. DOI: 10.1503/cmaj.051351.
HOOPER L V, LITTMAN D R, MACPHERSON A J. Interactions between the microbiota and the immune system[J]. Science, 2012, 336(6086): 1268-1273. DOI: 10.1126/science.1223490.
GILBERT J A, QUINN R A, DEBELIUS J, et al. Microbiome-wide association studies link dynamic microbial consortia to disease[J]. Nature, 2016, 535(7610): 94-103. DOI: 10.1038/nature18850.
BÄCKHED F, MANCHESTER J K, SEMENKOVICH C F, et al. Mechanisms underlying the resistance to diet-induced obesity in germ-free mice[J]. Proc Natl Acad Sci USA, 2007, 104(3): 979-984. DOI: 10.1073/pnas.0605374104.
SAMPSON T R, DEBELIUS J W, THRON T, et al. Gut Microbiota Regulate Motor Deficits and Neuroinflammation in a Model of Parkinson's Disease[J]. Cell, 2016, 167(6): 1469-1480.e12. DOI: 10.1016/j.cell.2016.11.018.
JOBIN C. GPR109a: the missing link between microbiome and good health[J]. Immunity, 2014, 40(1): 8-10. DOI:10.1016/j.immuni.2013.12.009.
MATSUMOTO M, INOUE R, TSUKAHARA T, et al. Voluntary running exercise alters microbiota composition and increases n-butyrate concentration in the rat cecum[J]. Biosci Biotechnol Biochem, 2008, 72(2): 572-576. DOI:10.1271/bbb.70474.
QUEIPO-ORTU O M I, SEOANE L M, MURRI M, et al. Gut microbiota composition in male rat models under different nutritional status and physical activity and its association with serum leptin and ghrelin levels[J]. PLoS ONE, 2013, 8(5): e65465. DOI: 10.1371/journal.pone.0065465.
CHOI J J, EUM S Y, RAMPERSAUD E, et al. Exercise Attenuates PCB-Induced Changes in the Mouse Gut Microbiome[J]. Environmental Health Perspectives, 2013, 121(6): 725-730. DOI: 10.1289/ehp.1306534.
EVANS C C, LEPARD K J, KWAK J W, et al. Exercise prevents weight gain and alters the gut microbiota in a mouse model of high fat diet-induced obesity[J]. PLoS ONE, 2014, 9(3): e92193. DOI: 10.1371/journal.pone.0092193.
CLARKE S F, MURPHY E F, O'SULLIVAN O, et al. Exercise and associated dietary extremes impact on gut microbial diversity[J]. Gut, 2014, 63(12): 1913-1920. DOI: 10.1136/gutjnl-2013-306541.
LIU T W, PARK Y M, HOLSCHER H D, et al. Physical Activity Differentially Affects the Cecal Microbiota of Ovariectomized Female Rats Selectively Bred for High and Low Aerobic Capacity[J]. Plos one, 2015, 10(8): e0136150. DOI: 10.1371/journal.pone.0136150.
ALMEIDA M L, FERINGER WH, CARVALHO J R, et al. Intense Exercise and Aerobic Conditioning Associated with Chromium or L-Carnitine Supplementation Modified the Fecal Microbiota of Fillies[J]. PLoS ONE, 2016, 11(12): e0167108. DOI: 10.1371/journal.pone.0167108.
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