米诺环素介导小胶质细胞形态转换减轻缺血性脑卒中早期脑损伤的机制研究

张波; 唐俊; 张建波; 杨立铭; 陈志; 杨云峰; 朱刚

doi:10.16016/j.1000-5404.201702080

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米诺环素介导小胶质细胞形态转换减轻缺血性脑卒中早期脑损伤的机制研究

基础医学 | 更新时间：2026-01-09

- 米诺环素介导小胶质细胞形态转换减轻缺血性脑卒中早期脑损伤的机制研究
- Minocycline conducted microglia phenotype transformation attenuates early brain injury in rats after ischemia stroke
- 第三军医大学学报 2017年39卷第11期页码：1105-1111
- 作者机构：
- 作者简介：
- 基金信息：
  
  面上项目(81571116);国家自然科学基金面上项目(81371440);国家重点基础研究发展计划(973计划);国家重点基础研究发展计划(2014CB541606)
- DOI：10.16016/j.1000-5404.201702080
  中图分类号：
- 收稿：2017-02-16，
  
  纸质出版：2017-06-15
- 稿件说明：
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张波, 唐俊, 张建波, 等. 米诺环素介导小胶质细胞形态转换减轻缺血性脑卒中早期脑损伤的机制研究[J]. 第三军医大学学报, 2017,39(11):1105-1111.

Bo hang, Jun Tang, Jianbo Zhang, et al. Minocycline conducted microglia phenotype transformation attenuates early brain injury in rats after ischemia stroke[J]. Journal of Third Military Medical University, 2017, 39(11): 1105-1111.
张波, 唐俊, 张建波, 等. 米诺环素介导小胶质细胞形态转换减轻缺血性脑卒中早期脑损伤的机制研究[J]. 第三军医大学学报, 2017,39(11):1105-1111. DOI： 10.16016/j.1000-5404.201702080.

Bo hang, Jun Tang, Jianbo Zhang, et al. Minocycline conducted microglia phenotype transformation attenuates early brain injury in rats after ischemia stroke[J]. Journal of Third Military Medical University, 2017, 39(11): 1105-1111. DOI： 10.16016/j.1000-5404.201702080.

摘要

Abstract

Objective

To investigate the early pathophysical characteristics of ischemic brain injury in rats

and explore the neuroprotective effects on the injury and possible involved molecular mechanisms.

Methods

Middle cerebral artery occlusion (MCAO) was performed in rats using a nylon suture to establish rat model of ischemic brain injury. In 6 and 24 h after MCAO

minocycline was injected intraperitoneally. Triphenyl tetrazolium chloride (TTC) staining was used to detect the infarct volume

and Tunel staining and Nissl staining were used to observe the apoptotic neurons. Immunohistochemical assay was used to detect microglial phenotype

and Western blot assay was employed to detect the expression of apoptosis pathway protein NF-κB. Morris water maze test was adopted to exam the long-term learning memory after MCAO and minocycline administration in 28 d after surgery.

Results

Minocycline administration significantly reduced brain infarct volume in 6 h after MCAO (43.0±5.3

36.0±6.8 cm

0.01)

decreased the apoptotic cerebral neurons and promoted M2 microglia proliferation. However

minocycline given in 24 h after MCAO had no protective effect on infarct volume and neuron apoptosis (

0.05). In addition

minocycline decreased NF-κB protein expression in 6 h after MCAO (

0.01). The learning and memory abilities were obviously lower in the rats after 28 d of MCAO

which could be notably improved by minocycline treatment (

0.01). But such neuroprotective effects were not observed in the rats treated 24 h after MCAO (

0.05).

Conclusion

Minocycline i

mproves memory deficits induced by early ischemic stroke

via

M2 microglial transformation and suppression of apoptosis signal pathway

and attenuates cerebral ischemia-induced neurological inflammation and apoptosis

so as to improve learning and memory function in rats.

关键词

Keywords

references

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