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时间分辨荧光微球免疫层析技术定量检测人血清胃蛋白酶原Ⅰ的实验研究
Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography
- 第三军医大学学报 2017年39卷第18期 页码：1803-1807
- 作者机构：
1.214063 江苏 无锡，江苏省原子医学研究所
2.214063 江苏 无锡，无锡市江原实业技贸总公司
- 作者简介：
[ 黄飚，E-mail：huangbiao@jsinm.org HUANG Biao, E-mail:huangbiao@jsinm.org
- 基金信息：江苏省科技支撑计划-社会发展(BE2014608);江苏省卫生计生委预防医学(Y2015072)
DOI：10.16016/j.1000-5404.201702048
中图分类号： R345.69;R446.112; R446.6收稿：2017-02-13，
修回：2017-05-10，
纸质出版：2017-09-30
- 稿件说明：
移动端阅览
周衍, 范俊, 蒋叶华, 等. 时间分辨荧光微球免疫层析技术定量检测人血清胃蛋白酶原Ⅰ的实验研究[J]. 第三军医大学学报, 2017,39(18):1803-1807.
Yan ZHOU, Jun FAN, Yehua JIANG, et al. Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography[J]. Journal of Third Military Medical University, 2017, 39(18): 1803-1807.
周衍, 范俊, 蒋叶华, 等. 时间分辨荧光微球免疫层析技术定量检测人血清胃蛋白酶原Ⅰ的实验研究[J]. 第三军医大学学报, 2017,39(18):1803-1807. DOI： 10.16016/j.1000-5404.201702048.
Yan ZHOU, Jun FAN, Yehua JIANG, et al. Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography[J]. Journal of Third Military Medical University, 2017, 39(18): 1803-1807. DOI： 10.16016/j.1000-5404.201702048.
摘要
目的
2
研制快速定量检测人血清胃蛋白酶原Ⅰ(pepsinogensⅠ，PGⅠ)试纸，并评价试纸性能。
方法
2
以包裹有时间分辨荧光的纳米微球作为标记示踪物，运用免疫层析试纸技术，研制PGⅠ检测试纸；考察试纸的检测线性、灵敏度、精密度、与对比试剂的符合率。
结果
2
试纸定量检测的线性良好，方程为Y=0.027 9+0.004 8X，
r
=0.997 8；灵敏度为0.27 μg/L；批内CV小于10%，批间CV 11.03%；与市售PGⅠ试剂平行检测489份血清样本，有良好的相关性(Y
TRFIA
=0.856 6X
strip
+7.545 6，
R
2
=0.989 1
P
<
0.01)。
结论
2
PGⅠ时间分辨荧光纳米微球免疫层析试纸操作简单、快速、经济、可定量，满足临床检测血清PGⅠ的需求。
Abstract
Objective
2
To develop a fast quantitative detection strip of pepsinogen Ⅰ (PGⅠ).
Methods
2
With the time-resolved fluorescent nanoparticles as the marker tracer
PGⅠ strip based on double-antibody sandwich immunochromatography was developed. The obtained strip was evaluated for its linearity
sensitivity
accuracy
and consistency with commercially available products (489 serum samples).
Results
2
The strip showed good linearity
and the linear equation was Y=0.027 9+0.004 8X
r
=0.997 8. The sensitivity of the PGⅠ strip was 0.27 μg/L. The coefficient of variation (CV) values of intra-batch was less than 10%
and that of inter-batch was 11.03%. Compared with the commercially available product (time-resolved fluorescence immunoassay)
our PGⅠ strip showed the high consistency (Y
TRFIA
=0.856 6X
strip
+7.545 6
R
2
=0.989 1
P
<
0.01).
Conclusion
2
Our developed time-resolved fluorescent nanoparticle immunochromatographic strip for PGⅠ detection is rapid
low cost
easy to operate
and quantitative
and can meet the needs of the clinical practice.
关键词
Keywords
references
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