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类鼻疽杆菌慢性感染BALB/c小鼠模型的建立
Establishment ofaBALB/c mouse model of melioidosis induced by chronic
Burkholderia pseudomallei infection- 第三军医大学学报 2017年39卷第12期 页码：1239-1244
- 作者机构：
400038重庆，第三军医大学西南医院医学检验系临床微生物及免疫学教研室
- 作者简介：
[毛旭虎, E-mail: mtf5225162@163.com Mao Xuhu, E-mail: mtf5225162@163.com
- 基金信息：国家自然科学基金面上项目(30973402)
DOI：10.16016/j.1000-5404.201701131
中图分类号： R-332;R378.99;R515.9收稿：2017-01-22，
修回：2017-02-28，
纸质出版：2017-06-30
- 稿件说明：
移动端阅览
马腾飞, 胡艺, 方瑶, 等. 类鼻疽杆菌慢性感染BALB/c小鼠模型的建立[J]. 第三军医大学学报, 2017,39(12):1239-1244.
Tengfei Ma, Yi Hu, Yao Fang, et al. Establishment ofaBALB/c mouse model of melioidosis induced by chronic
Burkholderia pseudomallei infection[J]. Journal of Third Military Medical University, 2017, 39(12): 1239-1244.马腾飞, 胡艺, 方瑶, 等. 类鼻疽杆菌慢性感染BALB/c小鼠模型的建立[J]. 第三军医大学学报, 2017,39(12):1239-1244. DOI： 10.16016/j.1000-5404.201701131.
Tengfei Ma, Yi Hu, Yao Fang, et al. Establishment ofaBALB/c mouse model of melioidosis induced by chronic
Burkholderia pseudomallei infection[J]. Journal of Third Military Medical University, 2017, 39(12): 1239-1244. DOI： 10.16016/j.1000-5404.201701131.
摘要
目的
2
建立类鼻疽杆菌(
Burkholderia pseudomallei
)慢性感染BALB/c小鼠模型并评价其稳定性。
方法
2
采用0.01 mL洗涤后的高、中、低剂量的类鼻疽杆菌液(菌量分别为3.3×10
4
、3.3×10
3
、3.3×10
2
CFU)滴鼻感染BALB/c小鼠，密切监测42 d，记录小鼠的生存率，探索合适的攻毒剂量。在低剂量细菌(3.3×10
2
CFU)感染小鼠后21、28、35、42 d，计数小鼠血液、肝脏、脾脏和肺脏的细菌定植量，ELISA检测血清炎性因子TNF-α、IFN-γ的表达和血清类鼻疽杆菌特异性IgG抗体滴度，观察肝脏、脾脏和肺脏等主要器官的病理学改变，评价动物模型的稳定性。
结果
2
低剂量类鼻疽杆菌滴鼻感染BALB/c小鼠后，小鼠42 d生存率为60%。期间小鼠体质量呈下降趋势，血液、肝脏、脾脏和肺脏均有细菌定植，并出现大量炎性细胞浸润、多灶性坏死和脓肿等病理改变；类鼻疽杆菌血清中特异性IgG抗体滴度从感染后21 d起持续增高；实验组小鼠血清炎性因子TNF-α和IFN-γ表达明显高于对照组(
P
<
0.05)。
结论
2
成功建立了稳定的类鼻疽杆菌慢性感染BALB/c小鼠模型。
Abstract
Objective
2
To establishaBALB/c mouse model of melioidosis induced by chronic
Burkholderia pseudomallei
(
B
.
pseudomallei
) infection.
Methods
2
BALB/c mice were challenged with different doses (3.3×10
4
3.3×10
3
and 3.3×10
2
CFU) of
B
.
pseudomallei
by intranasal instillation. The mice were monitored daily for 42dto record their survival rate and body weight changes. On days 21
28
35 and 42 following the infection
the bacterial loads in the blood and organs and the serum levels of tumor necrosis factor-α (TNF-α)
interferon-γ (IFN-γ) and specific anti-
B
.
pseudomallei
IgG were detected
and the pathological changes in the liver
panc
reas and lungs of the mice were assessed using HE staining.
Results
2
Intranasal challenge withalow dose (3.3×10
2
CFU) of
B
.
pseudomallei
resulted inasurvival rate of 60% in the mice on day 42. Chronic
B
.
pseudomallei
infection of the mice caused progressive body weight loss and induced obvious pathologies (inflammatory cell infiltration
multiple focal necrosis and abscess) in the liver
pancreas and lungs
where
B
.
pseudomallei
colonization was detected. The serum titer of specific
B
.
pseudomallei
IgG began to increase continuously from day 21 after the challenge
and the serum levels of TNF-α and IFN-γ were significantly higher in the infected mice than in the control mice.
Conclusion
2
We have successfully establishedamodel of chronic
B
.
pseudomallei
infection in BALB/c mice.
关键词
Keywords
references
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