大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响

吴栋栋; 徐伦山; 许民辉; 欧阳庆; 易良

doi:10.16016/j.1000-5404.201701109

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大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响

基础医学 | 更新时间：2026-01-09

- 大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响
- Effects of microglia and macrophages on migration of rat C6 glioma cells
- 第三军医大学学报 2017年39卷第11期页码：1117-1122
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金(81172413)
- DOI：10.16016/j.1000-5404.201701109
  中图分类号：
- 收稿：2017-01-19，
  
  纸质出版：2017-06-15
- 稿件说明：
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吴栋栋, 徐伦山, 许民辉, 等. 大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响[J]. 第三军医大学学报, 2017,39(11):1117-1122.

Dongdong Wu, Lunshan Xu, Minhui Xu, et al. Effects of microglia and macrophages on migration of rat C6 glioma cells[J]. Journal of Third Military Medical University, 2017, 39(11): 1117-1122.
吴栋栋, 徐伦山, 许民辉, 等. 大鼠小胶质细胞及巨噬细胞对胶质瘤C6细胞株迁移能力的影响[J]. 第三军医大学学报, 2017,39(11):1117-1122. DOI： 10.16016/j.1000-5404.201701109.

Dongdong Wu, Lunshan Xu, Minhui Xu, et al. Effects of microglia and macrophages on migration of rat C6 glioma cells[J]. Journal of Third Military Medical University, 2017, 39(11): 1117-1122. DOI： 10.16016/j.1000-5404.201701109.

摘要

Abstract

Objective

To determine the effect of microglia and peripheral blood macrophages on the migration of C6 glioma cells.

Methods

The rat microglial cells and macrophages differentiated from peripheral blood mononuclear cells were co-cultured with C6 glioma cells. Co-cultured with rat fibroblasts served as positive control and singly cultured C6 cells as blank control. Scratch wound healing assay was carried in 0

24 and 48 h after co-culture

and the results were observed in 6 h later. In 24 h and 48 h after co-culture

CD11b

flow cytometry was used to sort and isolate microglia/macrophages and C6 cells. The expression levels of IL-10

IL-18 and MMP-9 in microglia and macrophages and those of TGF-β and FAS ligand in glioma cells were detected by Western blotting when co-cultured for 0

24 and 48 h.

Results

At the initial state

both microglia and macrophages inhibited the migration of glioma

and the inhibitory effect of macrophage was stronger (

0.05). The tumor inhibitory effect of 2 kinds of cells disappeared after co-culture with glioma C6 cells

and all of them showed the effect of promoting tumor migration. The expression levels of IL-10

IL-18 and MMP-9 in the microglia and macrophages were different in the initial stage. But

the levels of these proteins were increased in these cells and consistent after co-culture. The expression levels of TGF-β and FAS ligand were also increased and consistent in glioma cells C6 after co-culture.

Conclusion

Microglia and macrophages are domesticated into the same glioma associated microglia/macrophages after co-culture with C6

and 2 kinds of cells show the same biological effects when co-culture with C6 cells.

关键词

Keywords

references

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