肺腺癌肿瘤相关巨噬细胞的免疫组织化学双标染色方法优化

张宪超; 张翔; 曹棉富; 陈露; 党微旗; 宋康; 崔有宏; 张厦; 卞修武

doi:10.16016/j.1000-5404.201701070

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肺腺癌肿瘤相关巨噬细胞的免疫组织化学双标染色方法优化

临床医学 | 更新时间：2026-01-09

- 肺腺癌肿瘤相关巨噬细胞的免疫组织化学双标染色方法优化
- Modification and improvement of immunohistochemical double staining for tumor associated macrophages in lung adenocarcinoma tissues
- 第三军医大学学报 2017年39卷第11期页码：1155-1158
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重点研发计划重点专项(2016YFC201801);国家自然科学基金面上项目(81372273);西南医院生物强院科技创新计划(SWH2016JCZD-04)
- DOI：10.16016/j.1000-5404.201701070
  中图分类号：
- 收稿：2017-01-11，
  
  纸质出版：2017-06-15
- 稿件说明：
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张宪超, 张翔, 曹棉富, 等. 肺腺癌肿瘤相关巨噬细胞的免疫组织化学双标染色方法优化[J]. 第三军医大学学报, 2017,39(11):1155-1158.

Xianchao Zhang, Xiang Zhang, Mianfu Cao, et al. Modification and improvement of immunohistochemical double staining for tumor associated macrophages in lung adenocarcinoma tissues[J]. Journal of Third Military Medical University, 2017, 39(11): 1155-1158.
张宪超, 张翔, 曹棉富, 等. 肺腺癌肿瘤相关巨噬细胞的免疫组织化学双标染色方法优化[J]. 第三军医大学学报, 2017,39(11):1155-1158. DOI： 10.16016/j.1000-5404.201701070.

Xianchao Zhang, Xiang Zhang, Mianfu Cao, et al. Modification and improvement of immunohistochemical double staining for tumor associated macrophages in lung adenocarcinoma tissues[J]. Journal of Third Military Medical University, 2017, 39(11): 1155-1158. DOI： 10.16016/j.1000-5404.201701070.

摘要

Abstract

Objective

To explore a stable and easy-to-identify color developing method by modifying immunohistochemical double-staining (D-IHC) so as to investigate the distribution of tumor associated macrophages (TAMs) in lung adenocarcinoma tissue.

Methods

Although as the same as the traditional double-enzyme methods which used the enzyme-conjugated secondary antibody to respectively link alkaline phosphatase (ALP) and horseradish peroxidase (HRP) as catalyze chromogenic substrates

AP-Red and diaminobenzidine (DAB) were chosen instead of 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP/NBT) and 3-Amino-9-ethylcarbazole (AEC) as chromogenic substrates. The distribution of TAMs in lung cancer tissues was observed under the microscope after the double staining were controlled in order.

Results

As the image was highly contrast and easily distinguishable with low background under a light microscope

Fast Red (first labeling) and DAB (second labeling) were suitable for application in D-IHC. In addition

these 2 kinds of color labeling were merged as brick red at the same position

which was recognizable under a light microscope. The statistical results revealed that M2 type of TAMs were priority (accounting for 80%) in the lung adenocarcinoma tissue samples.

Conclusion

The modified method is simple and reliable with stable labeling results

and will be useful in the exploration of lung adenocarcinoma and other biomedical research.

关键词

Keywords

references

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