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神经干细胞与大鼠RPE细胞间的线粒体转运及对RPE细胞凋亡的影响
Mitochondrial transfer from neural stem cells rescues apoptosis of retinal pigment epithelial cells isolated from RCS rats
- 第三军医大学学报 2017年39卷第11期 页码：1112-1116
- 作者机构：
- 作者简介：
- 基金信息：国家重点基础研究发展计划(973计划);国家重点基础研究发展计划(2013CB967002)
DOI：10.16016/j.1000-5404.201701031
中图分类号：收稿：2017-01-06，
纸质出版：2017-06-15
- 稿件说明：
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陈冲林, 邹婷, 黎其友, 等. 神经干细胞与大鼠RPE细胞间的线粒体转运及对RPE细胞凋亡的影响[J]. 第三军医大学学报, 2017,39(11):1112-1116.
Chonglin Chen, Ting Zou, Qiyou Li, et al. Mitochondrial transfer from neural stem cells rescues apoptosis of retinal pigment epithelial cells isolated from RCS rats[J]. Journal of Third Military Medical University, 2017, 39(11): 1112-1116.
陈冲林, 邹婷, 黎其友, 等. 神经干细胞与大鼠RPE细胞间的线粒体转运及对RPE细胞凋亡的影响[J]. 第三军医大学学报, 2017,39(11):1112-1116. DOI： 10.16016/j.1000-5404.201701031.
Chonglin Chen, Ting Zou, Qiyou Li, et al. Mitochondrial transfer from neural stem cells rescues apoptosis of retinal pigment epithelial cells isolated from RCS rats[J]. Journal of Third Military Medical University, 2017, 39(11): 1112-1116. DOI： 10.16016/j.1000-5404.201701031.
摘要
Abstract
Objective
2
To investigate the pattern of mitochondrial transfer in neural stem cells (NSCs)and primary cultured retinal pigment epithelial (RPE) cells isolated from Royal College of Surgeons (RCS) rats and analyze the effect mitochondrial transfer on the biological behaviors of RPE cells.
Methods
2
The RPE cells were isolated from the RCS rats
cultured primarily and identified. Mitotracker-red and mitotracker-green staining were used to label the mitochondria of the cultured RPE cells and those of mouse neural stem cell line C17.2
respectively.In a co-culture system of the 2 kinds of cells
the formation of tunneling nanotubes (TNT) between them and mitochondrial transfer in the TNT were observed by laser scanning confocal microscopy. Flow cytometry was used to measure the level of reactive oxygen species (ROS) production and analyze the cell cycle distribution and early apoptosis of RPE cells cultured alone or in the co-culture system.
Results
2
The RPE cel
ls in the third generation showed a good growth condition with the positive rates for RPE65 and Bestrophin both exceeding 95%. At 24 h after the co-culture
the formation of TNT structure was observed between RPE cells and NSCs
and one-way mitochondrial transfer from NSCs to RPE cells were captured. Mitochondrial transfer from the NSCs to the RPE cells resulted in significantly decreased ROS level in the latter cells (
P
<
0.05). The RPE cells receiving the mitochondria also showed enhanced cell proliferation
increased cell proportion in S phase (
P
<
0.01)
elevated proliferation index (
P
<
0.05)
and decreased early apoptosis cells(
P
<
0.05).
Conclusion
2
In the co-culture system
NSCs transfer mitochondria into adjacent RPE cells
via
the TNT structure to rescues the RPE cells from apoptosis.
关键词
Keywords
references
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