儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究

马廷廷; 黄延风; 杨震华; 苏薇

doi:10.16016/j.1000-5404.201701026

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儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究

临床医学 | 更新时间：2026-01-09

- 儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究
- Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children
- 第三军医大学学报 2017年39卷第17期页码：1768-1773
- 作者机构：
  
  1.400014重庆, 重庆医科大学附属儿童医院感染科, 儿童发育疾病研究教育部重点实验室, 儿童发育重大疾病国家国际科技合作基地, 儿科学重庆市重点实验室
  2.Department of Epidemiology, School of Public Health, University of Michigan, 1415 Washington Heights, Ann Arbor, MI 48109-2029, USA
- 作者简介：
  
  [黄延风, E-mail：ymhykq@126.com HUANG Yanfeng, E-mail: ymhykq@126.com
- 基金信息：
- DOI：10.16016/j.1000-5404.201701026
  中图分类号： R738.911;R394.3;R529.9
- 收稿：2017-01-05，
  
  修回：2017-04-18，
  
  纸质出版：2017-09-15
- 稿件说明：
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马廷廷, 黄延风, 杨震华, 等. 儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究[J]. 第三军医大学学报, 2017,39(17):1768-1773.

Tingting MA, Yanfeng HUANG, Zhenhua YANG, et al. Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children[J]. Journal of Third Military Medical University, 2017, 39(17): 1768-1773.
马廷廷, 黄延风, 杨震华, 等. 儿童结核病结核分枝杆菌临床分离株中esxA、fbpB、Rv2660c基因多态性研究[J]. 第三军医大学学报, 2017,39(17):1768-1773. DOI： 10.16016/j.1000-5404.201701026.

Tingting MA, Yanfeng HUANG, Zhenhua YANG, et al. Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children[J]. Journal of Third Military Medical University, 2017, 39(17): 1768-1773. DOI： 10.16016/j.1000-5404.201701026.

摘要

目的

对抗原编码基因esxA、fbpB、Rv2660c进行测序

了解esxA、fbpB、Rv2660c的基因多态性。

方法

选取135株结核分枝杆菌(

Mycobacterium tuberculosis

MTB)临床分离株

运用PCR方法扩增目的基因esxA、fbpB、Rv2660c并进行测序

与结核标准株H37Rv基因序列以及从免疫表位数据库(immune epitope database

IEDB)中查询到的表位序列进行对比

分析esxA、fbpB、Rv2660c基因序列中的变异特征。

结果

在135株菌株中

esxA、Rv2660c序列暂未发现基因变异。fbpB共66株(48.89%)发现了基因变异

5个单核苷酸多态性(single nucleotide polymorphism

SNP)位点共造成8个(14.81%)T细胞抗原表位以及9个(24.32%)B细胞抗原表位序列的多态性。fbpB基因的dN值为0.000 120

dS为0.002 126

其中T细胞表位区与非T细胞表位区的dN值分别为0.000 067和0.000 408

dS分别为0.002 382和0.000 470

B细胞表位区dN和dS值分别为0.000 063和0.002 380

非B细胞表位区dN和dS均为0。

结论

esxA、Rv2660c基因序列较保守

fbpB存在基因多态性

预测会对蛋白Ag85B的免疫功能造成影响

从而影响H56的免疫有效性。

Abstract

Objective

To sequence the antigen-encoding genes esxA

fbpB and Rv2660c from clinical isolates of

Mycobacterium tuberculosis

(MTB) in tuberculosis children and study the polymorphisms.

Methods

A total of 135 isolated strains were amplified for gene sequences of esxA

fbpB and Rv2660c by PCR

and the products were sequenced respectively. The results of sequencing were compared with the corresponding gene sequences of standard strain H37Rv and human T and B cell epitope sequences from immune epitope database (IEDB). The genetic diversities of the gene sequences of esxA

fbpB and Rv2660c were analyzed.

Results

Among the 135 strains

no gene polymorphisms were found in esxA and Rv2660c. But fbpB had genetic diversities in 66 clinical isolates (48.89%). The 5 single nucleotide polymorphism (SNP) mutations in fbpB affected 8 (14.81%) T cell epitopes and 9 (24.32%) B cell epitopes. The dN and dS values of whole fbpB gene were 0.000 120 and 0.002 126

respectively. The dN and dS values of T epitopes areas were 0.000 067 and 0.002 382 respectively

those of non-T epitopes area were 0.000 408 and 0.000 470

respectively

those of B epitopes areas were 0.000 063 and 0.00 2380

and those of non-B epitopes areas were 0.

Conclusion

The gene sequences of esxA and Rv2660c are conservative

and that of fbpB contains genetic diversities. Its polymorphisms may influence the function of antigen Ag85B

and thus affect the efficacy of vaccine H56.

关键词

Keywords

references

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