CD33<sup>low</sup>CD11b<sup>low</sup>髓系细胞在肝癌患者中的表型、形态与功能

朱雯洁; 林洁; 郑利民

doi:10.16016/j.1000-5404.201701018

您当前的位置：

首页 >

文章列表页 >

CD33^lowCD11b^low髓系细胞在肝癌患者中的表型、形态与功能

临床医学 | 更新时间：2026-01-09

- CD33^lowCD11b^low髓系细胞在肝癌患者中的表型、形态与功能
- Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients
- 第三军医大学学报 2017年39卷第11期页码：1137-1143
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金重大研究计划(91442205)
- DOI：10.16016/j.1000-5404.201701018
  中图分类号：
- 收稿：2017-01-04，
  
  纸质出版：2017-06-15
- 稿件说明：
移动端阅览
朱雯洁, 林洁, 郑利民. CD33^lowCD11b^low髓系细胞在肝癌患者中的表型、形态与功能[J]. 第三军医大学学报, 2017,39(11):1137-1143.

Wenjie Zhu, Jie Lin, Limin Zheng. Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients[J]. Journal of Third Military Medical University, 2017, 39(11): 1137-1143.
朱雯洁, 林洁, 郑利民. CD33^lowCD11b^low髓系细胞在肝癌患者中的表型、形态与功能[J]. 第三军医大学学报, 2017,39(11):1137-1143. DOI： 10.16016/j.1000-5404.201701018.

Wenjie Zhu, Jie Lin, Limin Zheng. Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients[J]. Journal of Third Military Medical University, 2017, 39(11): 1137-1143. DOI： 10.16016/j.1000-5404.201701018.

摘要

Abstract

Objective

To investigate the phenotype

morphology

and function of CD33

low

CD11b

low

myeloid cells in the patients with hepatocellular carcinoma (HCC).

Methods

The phenotype and morphology of CD33

low

CD11b

low

myeloid cells were examined using flow cytometry and Wright-Giemsa staining. The immune functions of CD33

low

CD11b

low

myeloid cells were tested

via

T cell co-culture assay

using freshly isolated CD33

low

CD11b

low

myeloid cells from HCC tissues or tumor supernatant-treated blood CD33

low

CD11b

low

myeloid cells from healthy donors.

Results

Aside from canonical polymorphonuclear neutrophils

there was a subset of CD33

low

CD11b

low

granulocytes in the peripheral blood and tumor tissues in the HCC patients. These cells expressed CD123

CD38 and a low level of HLA-DR

but not CD15

CD16

or CD66b

which resembled a phenotype of basophils. Interestingly

these cells exhibited an immature morphology in HCC patients (

0.001). The frequencies of blood CD33

low

CD11b

low

myeloid cells were significantly higher in the HCC patients than the healthy donors

and these cells were enriched in the tumors. HCC-conditioned CD33

low

CD11b

low

myeloid cells had profound capacity in fostering T cell proliferation.

Conclusion

CD33

low

CD11b

low

myeloid cells

a subset of myeloid cells phenotypically resembled basophils

accumulate in the HCC patients and exhibit an immature morphology

which may contribute to anti-tumor immune response in the tumor microenvironment.

关键词

Keywords

references

Llovet J M Zucman-Rossi J Pikarsky E Hepatocellular carcinoma Nat Rev Dis Primers 2016 2 1 23 10.1038/nrdp.2016.18 Llovet J M, Zucman-Rossi J, Pikarsky E, et al .Hepatocellular carcinoma[J ] .Nat Rev Dis Primers, 2016, 2: 1-23.DOI:10.1038/nrdp.2016.18.

Torre L A Bray F Siegel R L Global cancer statistics, 2012 CA Cancer J Clin 2015 65 2 87 108 10.3322/caac.21262 Torre L A, Bray F, Siegel R L, et al .Global cancer statistics, 2012[J ] .CA Cancer J Clin, 2015, 65(2): 87-108.DOI:10.3322/caac.21262.

Tian Y Yang W Song J Hepatitis B virus X protein-induced aberrant epigenetic modifications contributing to human hepatocellular carcinoma pathogenesis Mol Cell Biol 2013 33 15 2810 2816 10.1128/MCB.00205-13 Tian Y, Yang W, Song J, et al .Hepatitis B virus X protein-induced aberrant epigenetic modifications contributing to human hepatocellular carcinoma pathogenesis[J ] .Mol Cell Biol, 2013, 33(15): 2810-2816.DOI:10.1128/MCB.00205-13.

Wu S Yang C Guo S Stimulation of B7-H1 in hepatocarcinoma cells by hepatitis B virus X antigen Immunol Invest 2010 39 7 754 769 10.3109/08820139.2010.494193 Wu S, Yang C, Guo S, et al .Stimulation of B7-H1 in hepatocarcinoma cells by hepatitis B virus X antigen[J ] .Immunol Invest, 2010, 39(7): 754-769.DOI:10.3109/08820139.2010.494193.

Alison M R , Nicholson L J , Lin W R . Chronic inflammation and hepatocellular carcinoma [M ] // Inflammation and Gastrointestinal Cancers , 2011 , 185 : 135 - 148 .DOI: 10.1007/978-3-642-03503-6_8. Alison M R, Nicholson L J, Lin W R.Chronic inflammation and hepatocellular carcinoma[M]//Inflammation and Gastrointestinal Cancers, 2011, 185: 135-148.DOI:10.1007/978-3-642-03503-6_8.

Hanahan D Weinberg R A Hallmarks of cancer: the next generation Cell 2011 144 5 646 674 10.1016/j.cell.2011.02.013 Hanahan D, Weinberg R A.Hallmarks of cancer: the next generation[J].Cell, 2011, 144(5): 646-674.DOI:10.1016/j.cell.2011.02.013.

Kuang D M Zhao Q Peng C Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1 J Exp Med 2009 206 6 1327 1337 10.1084/jem.20082173 Kuang D M, Zhao Q, Peng C, et al .Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1[J ] .J Exp Med, 2009, 206(6): 1327-1337.DOI:10.1084/jem.20082173.

Li X F Chen D P Ouyang F Z Increased autophagy sustains the survival and pro-tumourigenic effects of neutrophils in human hepatocellular carcinoma J Hepatol 2015 62 1 131 139 10.1016/j.jhep.2014.08.023 Li X F, Chen D P, Ouyang F Z, et al .Increased autophagy sustains the survival and pro-tumourigenic effects of neutrophils in human hepatocellular carcinoma[J ] .J Hepatol, 2015, 62(1): 131-139.DOI:10.1016/j.jhep.2014.08.023.

Ouyang F Z Wu R Q Wei Y Dendritic cell-elicited B-cell activation fosters immune privilege via IL-10 signals in hepatocellular carcinoma Nat Commun 2016 7 13453 10.1038/ncomms13453 Ouyang F Z, Wu R Q, Wei Y, et al .Dendritic cell-elicited B-cell activation fosters immune privilege via IL-10 signals in hepatocellular carcinoma[J ] .Nat Commun, 2016, 7: 13453.DOI:10.1038/ncomms13453.

Engblom C Pfirschke C Pittet M J The role of myeloid cells in cancer therapies Nat Rev Cancer 2016 16 7 447 462 10.1038/nrc.2016.54 Engblom C, Pfirschke C, Pittet M J.The role of myeloid cells in cancer therapies[J].Nat Rev Cancer, 2016, 16(7): 447-462.DOI:10.1038/nrc.2016.54.

Kumar V Patel S Tcyganov E The nature of myeloid-derived suppressor cells in the tumor microenvironment Trends Immunol 2016 37 3 208 220 10.1016/j.it.2016.01.004 Kumar V, Patel S, Tcyganov E, et al .The nature of myeloid-derived suppressor cells in the tumor microenvironment[J ] .Trends Immunol, 2016, 37(3): 208-220.DOI: 10.1016/j.it.2016.01.004.

Luo B Gan W Liu Z Erythropoeitin signaling in macrophages promotes dying cell clearance and immune tolerance Immunity 2016 44 2 287 302 10.1016/j.immuni.2016.01.002 Luo B, Gan W, Liu Z, et al .Erythropoeitin signaling in macrophages promotes dying cell clearance and immune tolerance[J ] .Immunity, 2016, 44(2):287-302.DOI: 10.1016/j.immuni.2016.01.002.

Powell D R Huttenlocher A Neutrophils in the tumor microenvironment Trends Immunol 2016 37 1 41 52 10.1016/j.it.2015.11.008 Powell D R, Huttenlocher A.Neutrophils in the tumor microenvironment[J].Trends Immunol, 2016, 37(1): 41-52.DOI:10.1016/j.it.2015.11.008.

Coffelt S B Wellenstein M D de Visser K E Neutrophils in cancer: neutral no more Nat Rev Cancer 2016 16 7 431 446 10.1038/nrc.2016.52 Coffelt S B, Wellenstein M D, de Visser K E.Neutrophils in cancer: neutral no more[J].Nat Rev Cancer, 2016, 16(7): 431-446.DOI:10.1038/nrc.2016.52.

Peranzoni E Zilio S Marigo I Myeloid-derived suppressor cell heterogeneity and subset definition Curr Opin Immunol 2010 22 2 238 244 10.1016/j.coi.2010.01.021 Peranzoni E, Zilio S, Marigo I, et al .Myeloid-derived suppressor cell heterogeneity and subset definition[J ] .Curr Opin Immunol, 2010, 22(2): 238-244.DOI:10.1016/j.coi.2010.01.021.

Sionov R V Fridlender Z G Granot Z The Multifaceted Roles Neutrophils Play in the Tumor Microenvironment Cancer Microenviron 2015 8 3 125 158 10.1007/s12307-014-0147-5 Sionov R V, Fridlender Z G, Granot Z.The Multifaceted Roles Neutrophils Play in the Tumor Microenvironment[J].Cancer Microenviron, 2015, 8(3): 125-158.DOI:10.1007/s12307-014-0147-5.

Kushner B H Cheung N K Absolute requirement of CD11/CD18 adhesion molecules, FcRⅡ and the phosphatidylinositol-linked FcRⅢ for monoclonal antibody-mediated neutrophil antihuman tumor cytotoxicity Blood 1992 79 6 1484 1490 http://www.bloodjournal.org/content/79/6/1484.short Kushner B H, Cheung N K.Absolute requirement of CD11/CD18 adhesion molecules, FcRⅡ and the phosphatidylinositol-linked FcRⅢ for monoclonal antibody-mediated neutrophil antihuman tumor cytotoxicity[J].Blood, 1992, 79(6): 1484-1490.

Granot Z Henke E Comen E A Tumor entrained neutrophils inhibit seeding in the premetastatic lung Cancer Cell 2011 20 3 300 314 10.1016/j.ccr.2011.08.012 Granot Z, Henke E, Comen E A, et al .Tumor entrained neutrophils inhibit seeding in the premetastatic lung[J ] .Cancer Cell, 2011, 20(3): 300-314.DOI:10.1016/j.ccr.2011.08.012.

López-Lago M A Posner S Thodima V J Neutrophil chemokines secreted by tumor cells mount a lung antimetastatic response during renal cell carcinoma progression Oncogene 2013 32 14 1752 1760 10.1038/onc.2012.201 López-Lago M A, Posner S, Thodima V J, et al .Neutrophil chemokines secreted by tumor cells mount a lung antimetastatic response during renal cell carcinoma progression[J ] .Oncogene, 2013, 32(14): 1752-1760.DOI:10.1038/onc.2012.201.

Strauss L Sangaletti S Consonni F M RORC1 Regulates Tumor-Promoting "Emergency" Granulo-Monocytopoiesis Cancer Cell 2015 28 2 253 269 10.1016/j.ccell.2015.07.006 Strauss L, Sangaletti S, Consonni F M, et al .RORC1 Regulates Tumor-Promoting "Emergency" Granulo-Monocytopoiesis[J ] .Cancer Cell, 2015, 28(2): 253-269.DOI:10.1016/j.ccell.2015.07.006.

Chirumbolo S Ortolani R Vella A CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: some comments Cytometry A 2011 79 2 102 106 10.1002/cyto.a.21008 Chirumbolo S, Ortolani R, Vella A.CCR3 as a single selection marker compared to CD123/HLADR to isolate basophils in flow cytometry: some comments[J].Cytometry A, 2011, 79(2): 102-106.DOI:10.1002/cyto.a.21008.

Singhal S Bhojnagarwala P S O'Brien S Origin and Role of a Subset of Tumor-Associated Neutrophils with Antigen-Presenting Cell Features in Early-Stage Human Lung Cancer Cancer Cell 2016 30 1 120 135 10.1016/j.ccell.2016.06.001 Singhal S, Bhojnagarwala P S, O'Brien S, et al .Origin and Role of a Subset of Tumor-Associated Neutrophils with Antigen-Presenting Cell Features in Early-Stage Human Lung Cancer[J ] .Cancer Cell, 2016, 30(1): 120-135.DOI:10.1016/j.ccell.2016.06.001.

Condamine T Dominguez G A Youn J I Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients Science Immunology 2016 1 2 10.1126/sciimmunol.aaf8943 Condamine T, Dominguez G A, Youn J I, et al .Lectin-type oxidized LDL receptor-1 distinguishes population of human polymorphonuclear myeloid-derived suppressor cells in cancer patients[J ] .Science Immunology, 2016, 1(2): aaf8943.DOI:10.1126/sciimmunol.aaf8943.

Sagiv J Y Michaeli J Assi S Phenotypic diversity and plasticity in circulating neutrophil subpopulations in cancer Cell Rep 2015 10 4 562 573 10.1016/j.celrep.2014.12.039 Sagiv J Y, Michaeli J, Assi S, et al .Phenotypic diversity and plasticity in circulating neutrophil subpopulations in cancer[J ] .Cell Rep, 2015, 10(4): 562-573.DOI:10.1016/j.celrep.2014.12.039

Ueha S Shand F H Matsushima K Myeloid cell population dynamics in healthy and tumor-bearing mice Int Immunopharmacol 2011 11 7 783 788 10.1016/j.intimp.2011.03.003 Ueha S, Shand F H, Matsushima K.Myeloid cell population dynamics in healthy and tumor-bearing mice[J].Int Immunopharmacol, 2011, 11(7): 783-788.DOI:10.1016/j.intimp.2011.03.003.

Condamine T Mastio J Gabrilovich D I Transcriptional regulation of myeloid-derived suppressor cells J Leukoc Biol 2015 98 6 913 922 10.1189/jlb.4RI0515-204R Condamine T, Mastio J, Gabrilovich D I.Transcriptional regulation of myeloid-derived suppressor cells[J].J Leukoc Biol, 2015, 98(6): 913-922.DOI:10.1189/jlb.4RI0515-204R.

Talmadge J E Gabrilovich D I History of myeloid-derived suppressor cells Nat Rev Cancer 2013 13 10 739 752 10.1038/nrc3581 https://www.researchgate.net/profile/James_Talmadge/publication/256984027_History_of_myeloid-derived_supressor_cells/links/00b495266b0f25534e000000/History-of-myeloid-derived-supressor-cells.pdf Talmadge J E, Gabrilovich D I.History of myeloid-derived suppressor cells[J].Nat Rev Cancer, 2013, 13(10): 739-752.DOI:10.1038/nrc3581.

王婷婷庄园陈娜肿瘤相关中性粒细胞在胃癌中的分布、表型及其免疫抑制功能研究第三军医大学学报 2014 36 6 587 591 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201309202 王婷婷, 庄园, 陈娜, 等.肿瘤相关中性粒细胞在胃癌中的分布、表型及其免疫抑制功能研究[J].第三军医大学学报, 2014, 36(6):587-591.

Wang T T Zhuang Y Chen N Distribution, phenotype and immunosuppressive function of tumor-associated neutrophils in gastric cancer J Third Mil Med Univ 2014 36 6 587 591 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201309202 Wang T T, Zhuang Y, Chen N, et al .Distribution, phenotype and immunosuppressive function of tumor-associated neutrophils in gastric cancer[J ] .J Third Mil Med Univ, 2014, 36(6):587-591.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

高盐饮食抑制小鼠NK细胞成熟和杀伤功能的研究

CD33lowCD11blow髓系细胞在肝癌患者中的表型、形态与功能

Phenotype, morphology and function of hepatocellular carcinoma-associated CD33lowCD11blow myeloid cells in hepatocellular carcinoma patients

DOI：10.16016/j.1000-5404.201701018

摘要

Abstract

关键词

Keywords

references

CD33^lowCD11b^low髓系细胞在肝癌患者中的表型、形态与功能

Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients