miRNA-671上调抑制胃癌细胞增殖与迁移

唐佳佳; 叶林

doi:10.16016/j.1000-5404.201612169

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miRNA-671上调抑制胃癌细胞增殖与迁移

基础医学 | 更新时间：2026-01-09

- miRNA-671上调抑制胃癌细胞增殖与迁移
- Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells
- 第三军医大学学报 2017年39卷第16期页码：1643-1647
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201612169
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- 收稿：2016-12-24，
  
  纸质出版：2017-08-30
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唐佳佳, 叶林. miRNA-671上调抑制胃癌细胞增殖与迁移[J]. 第三军医大学学报, 2017,39(16):1643-1647.

Jiajia TANG, Lin YE. Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells[J]. Journal of Third Military Medical University, 2017, 39(16): 1643-1647.
唐佳佳, 叶林. miRNA-671上调抑制胃癌细胞增殖与迁移[J]. 第三军医大学学报, 2017,39(16):1643-1647. DOI： 10.16016/j.1000-5404.201612169.

Jiajia TANG, Lin YE. Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells[J]. Journal of Third Military Medical University, 2017, 39(16): 1643-1647. DOI： 10.16016/j.1000-5404.201612169.

摘要

Abstract

Objective

To investigate the expression of miR-671 in gastric cancer tissues and cell lines

and determine its effects on the proliferation and invasion of gastric cancer cells.

Methods

The expression of miR-671 was detected in gastric cancer tissues and para-carcerous tissues from 8 patients and cell lines SGC7901

MKN28 and MKN45

and human gastric mucosal epithelial cell line GES-1 by quantitative real-time PCR (qRT-PCR). CCK-8 assay was used to measure the cell proliferation after miR-671 up-regulation. The effect of miR-671 on cell migration and invasion was detected by wound healing assay and Transwell chamber assay.

Results

The expression level of miR-671 was significantly decreased in gastric cancer tissues than the adjacent normal tissues (

0.01). The expression levels of miR-671 were significantly decreased in the gastric cancer cell lines compared with GES-1 cells (

0.01). Up-regulation of miR-671 significantly inhibited the proliferation

migration and invasion in gastric cancer cells (

0.01).

Conclusion

miR-671 inhibits proliferation

migration and invasion of gastric cancer cells

and may serve as a tumor suppressor.

关键词

Keywords

references

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