二氢杨梅素改善ob/ob小鼠心脏功能及其相关作用机制研究

公欣华; 周启程; 顾业芸; 朱俊东; 糜漫天

doi:10.16016/j.1000-5404.201612114

您当前的位置：

首页 >

文章列表页 >

二氢杨梅素改善ob/ob小鼠心脏功能及其相关作用机制研究

基础医学 | 更新时间：2026-01-09

- 二氢杨梅素改善ob/ob小鼠心脏功能及其相关作用机制研究
- Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism
- 第三军医大学学报 2017年39卷第16期页码：1612-1617
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81372975)
- DOI：10.16016/j.1000-5404.201612114
  中图分类号：
- 收稿：2016-12-15，
  
  纸质出版：2017-08-30
- 稿件说明：
移动端阅览
公欣华, 周启程, 顾业芸, 等. 二氢杨梅素改善ob/ob小鼠心脏功能及其相关作用机制研究[J]. 第三军医大学学报, 2017,39(16):1612-1617.

Xinhua GONG, Qicheng ZHOU, Yeyun GU, et al. Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(16): 1612-1617.
公欣华, 周启程, 顾业芸, 等. 二氢杨梅素改善ob/ob小鼠心脏功能及其相关作用机制研究[J]. 第三军医大学学报, 2017,39(16):1612-1617. DOI： 10.16016/j.1000-5404.201612114.

Xinhua GONG, Qicheng ZHOU, Yeyun GU, et al. Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(16): 1612-1617. DOI： 10.16016/j.1000-5404.201612114.

摘要

Abstract

Objective

To determine the protective effect of dihydromyricetin (DHM) on cardiac function in ob/ob mice (with leptin mutation) and investigate the underlying mechanism.

Methods

Thirty-six male ob/ob mice were randomly divided into 3 groups: ob/ob group

ob/ob+DHM 50 mg/kg group

ob/ob+DHM 100 mg/kg group (

=12). Ten C57BL/6J wild type mice are used as normal control (WT). Glucose tolerance and insulin tolerance were measured after 16 weeks' treatment. Cardiac function was detected by echocardiography. Myocardial histopathology and collagen deposition were detected by HE and Masson staining. The protein expression levels o

f peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and fibronectin typeⅢ domain-containing protein 5 (FNDC5) in the myocardium were detected by Western blotting. The expression of FNDC5 in the myocardium was observed by immunohistochemical assay.

Results

Glucose tolerance and insulin tolerance were obviously injured in the ob/ob mice than the WT group (

0.05). In the ob/ob mice

no changes were found in diastolic left ventricular diameter (LVIDd)

systolic left ventricular diameter (LVIDs)

or ratio of velocity of early filling wave (E) to velocity of late filling wave due to atrial contraction (A) (

0.05)

or myocardial hypertrophy or collagen deposition

but notable decreases were found in short axis fractional shortening (FS) and ejection fraction (EF) (

0.05)

as well as the protein expression levels of PGC-1α and FNDC5 (

0.05). DHM treatment for 16 weeks could significantly reverse the above changes in the ob/ob mice (

0.05).

Conclusion

DHM ameliorates cardiac function in the ob/ob mice

which might be related to PGC-1α/FNDC5 signalling pathway.

关键词

Keywords

references

INGELSSON E SUNDSTRÖM J ARNLÖV J Insulin resistance and risk of congestive heart failure JAMA 2005 294 3 334 341 10.1001/jama.294.3.334 INGELSSON E, SUNDSTRÖM J, ARNLÖV J, et al . Insulin resistance and risk of congestive heart failure[J ] . JAMA, 2005, 294(3): 334-341. DOI:10.1001/jama.294.3.334.

SHEN Y LINDEMEYER A K GONZALEZ C Dihydromyricetin as a novel anti-alcohol intoxication medication J Neurosci 2012 32 1 390 401 10.1523/JNEUROSCI.4639-11.2012 SHEN Y, LINDEMEYER A K, GONZALEZ C, et al . Dihydromyricetin as a novel anti-alcohol intoxication medication[J ] . J Neurosci, 2012, 32(1): 390-401.DOI:10.1523/JNEUROSCI.4639-11.2012.

XIA J GUO S FANG T Dihydromyricetin induces autophagy in HepG2 cells involved in inhibition of mTOR and regulating its upstream pathways Food Chem Toxicol 2014 66 7 13 10.1016/j.fct.2014.01.014 XIA J, GUO S, FANG T, et al . Dihydromyricetin induces autophagy in HepG2 cells involved in inhibition of mTOR and regulating its upstream pathways[J ] . Food Chem Toxicol, 2014, 66: 7-13.DOI:10.1016/j.fct.2014.01.014.

ZHANG Q LIU J LIU B Dihydromyricetin promotes hepatocellular carcinoma regression via a p53 activation-dependent mechanism Sci Rep 2014 4 4628 10.1038/srep04628 ZHANG Q, LIU J, LIU B, et al . Dihydromyricetin promotes hepatocellular carcinoma regression via a p53 activation-dependent mechanism[J ] . Sci Rep, 2014, 4: 4628.DOI:10.1038/srep04628.

WU S LIU B ZHANG Q Dihydromyricetin reduced Bcl-2 expression via p53 in human hepatoma HepG2 cells PLoS One 2013 8 11 e76886 10.1371/journal.pone.0076886 WU S, LIU B, ZHANG Q, et al . Dihydromyricetin reduced Bcl-2 expression via p53 in human hepatoma HepG2 cells[J ] . PLoS One, 2013, 8(11): e76886. DOI:10.1371/journal.pone.0076886.

LIAO W NING Z MA L Recrystallization of dihydromyricetin from Ampelopsis grossedentata and its anti-oxidant activity evaluation Rejuvenation Res 2014 17 5 422 429 10.1089/rej.2014.1555 LIAO W, NING Z, MA L, et al . Recrystallization of dihydromyricetin from Ampelopsis grossedentata and its anti-oxidant activity evaluation[J ] . Rejuvenation Res, 2014, 17(5): 422-429.DOI:10.1089/rej.2014.1555.

SHI L ZHANG T LIANG X Dihydromyricetin improves skeletal muscle insulin resistance by inducing autophagy via the AMPK signaling pathway Mol Cell Endocrinol 2015 409 92 102 10.1016/j.mce.2015.03.009 SHI L, ZHANG T, LIANG X, et al . Dihydromyricetin improves skeletal muscle insulin resistance by inducing autophagy via the AMPK signaling pathway[J ] . Mol Cell Endocrinol, 2015, 409: 92-102. DOI:10.1016/j.mce.2015.03.009.

WRANN C D WHITE J P SALOGIANNNIS J Exercise induces hippocampal BDNF through a PGC-1α/FNDC5 pathway Cell Metab 2013 18 5 649 659 10.1016/j.cmet.2013.09.008 WRANN C D, WHITE J P, SALOGIANNNIS J, et al . Exercise induces hippocampal BDNF through a PGC-1α/FNDC5 pathway[J ] . Cell Metab, 2013, 18(5): 649-659. DOI:10.1016/j.cmet.2013.09.008.

WITTELES R M FOWLER M B Insulin-resistant cardiomyopathy clinical evidence, mechanisms, and treatment options J Am Coll Cardiol 2008 51 2 93 102 10.1016/j.jacc.2007.10.021 WITTELES R M, FOWLER M B. Insulin-resistant cardiomyopathy clinical evidence, mechanisms, and treatment options[J]. J Am Coll Cardiol, 2008, 51(2): 93-102. DOI:10.1016/j.jacc.2007.10.021.

FANG Z Y PRINS J B MARWICK T H Diabetic cardiomyopathy: evidence, mechanisms, and therapeutic implications Endocr Rev 2004 25 4 543 567 10.1210/er.2003-0012. FANG Z Y, PRINS J B, MARWICK T H. Diabetic cardiomyopathy: evidence, mechanisms, and therapeutic implications[J]. Endocr Rev, 2004, 25(4): 543-567.DOI:10.1210/er.2003-0012.

张平郭莹张宇 PGC-1α与心脏生理和疾病关系的研究进展山东医药 2012 52 28 88 90, 93 10.3969/j.issn.1002-266X.2012.28.037 张平, 郭莹, 张宇, 等. PGC-1α与心脏生理和疾病关系的研究进展[J].山东医药, 2012, 52(28): 88-90, 93. DOI: 10.3969/j.issn.1002-266X.2012.28.037.

ZHANG P GUO Y ZHANG Y Progress in the relation between PGC-1α and cardiac physiology and diseases Shandong Med J 2012 52 28 88 90, 93 10.3969/j.issn.1002-266X.2012.28.037 ZHANG P, GUO Y, ZHANG Y, et al . Progress in the relation between PGC-1α and cardiac physiology and diseases[J ] . Shandong Med J, 2012, 52(28): 88-90, 93. DOI:10.3969/j.issn.1002-266X.2012.28.037.

WANG Q LI C ZHANG Q The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway BMC Complement Altern Med 2016 16 1 514 10.1186/s12906-016-1496-z WANG Q, LI C, ZHANG Q, et al . The effect of Chinese herbs and its effective components on coronary heart disease through PPARs-PGC1α pathway[J ] . BMC Complement Altern Med, 2016, 16(1): 514. DOI:10.1186/s12906-016-1496-z.

XU X YING Z CAI M Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue AJP: Regulatory, Integrative and Comparative Physiology 2011 300 5 R1115 R1125 10.1152/ajpregu.00806.2010 XU X, YING Z, CAI M, et al . Exercise ameliorates high-fat diet-induced metabolic and vascular dysfunction, and increases adipocyte progenitor cell population in brown adipose tissue[J ] . AJP: Regulatory, Integrative and Comparative Physiology, 2011, 300(5): R1115-R1125. DOI:10.1152/ajpregu.00806.2010.

ZADEGAN F G GHAEDI K KALANTAR S M Cardiac differentiation of mouse embryonic stem cells is influenced by a PPAR γ/PGC-1α-FNDC5 pathway during the stage of cardiac precursor cell formation Eur J Cell Biol 2015 94 6 257 266 10.1016/j.ejcb.2015.04.002 ZADEGAN F G, GHAEDI K, KALANTAR S M, et al . Cardiac differentiation of mouse embryonic stem cells is influenced by a PPAR γ /PGC-1α-FNDC5 pathway during the stage of cardiac precursor cell formation[J ] . Eur J Cell Biol, 2015, 94(6): 257-266. DOI:10.1016/j.ejcb.2015.04.002.

AYDIN S KULOGLU T AYDIN S Cardiac, skeletal muscle and serum irisin responses to with or without water exercise in young and old male rats: cardiac muscle produces more irisin than skeletal muscle Peptides 2014 52 68 73 10.1016/j.peptides.2013.11.024 AYDIN S, KULOGLU T, AYDIN S, et al . Cardiac, skeletal muscle and serum irisin responses to with or without water exercise in young and old male rats: cardiac muscle produces more irisin than skeletal muscle[J ] . Peptides, 2014, 52: 68-73.DOI:10.1016/j.peptides.2013.11.024.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

藤茶提取物改善2型糖尿病大鼠胰岛素抵抗的作用

二氢杨梅素改善糖尿病肾病的关键靶分子的筛选与验证

二氢杨梅素通过Prohibitin 2增强血管内皮细胞线粒体自噬

粪便细菌移植对非酒精性脂肪性肝病大鼠肝功能和脂代谢紊乱的影响

二氢杨梅素通过抑制mTOR信号通路抑制乳腺癌细胞迁移和侵袭