6-磷酸果糖-2-激酶在结直肠癌中的表达及临床意义

魏莱; 王喆; 梁后杰

doi:10.16016/j.1000-5404.201612084

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6-磷酸果糖-2-激酶在结直肠癌中的表达及临床意义

更新时间：2026-02-09

- 6-磷酸果糖-2-激酶在结直肠癌中的表达及临床意义
- Expression of 6-phosphofructokinase-2 in colorectal cancer and its clinical significance
- 第三军医大学学报 2017年39卷第10期页码：983-989
- 作者机构：
  
  400038 重庆，第三军医大学西南医院肿瘤科
- 作者简介：
  
  [梁后杰, E-mail:lianghoujie@sina.com.cn Liang Houjie, E-mail: lianghoujie@sina.com.cn
- 基金信息：
  
  国家自然科学基金面上项目(81472698)
- DOI：10.16016/j.1000-5404.201612084
  中图分类号： R345.57;R730.23;R735.35
- 收稿：2016-12-12，
  
  修回：2017-02-06，
  
  纸质出版：2017-05-30
- 稿件说明：
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魏莱, 王喆, 梁后杰. 6-磷酸果糖-2-激酶在结直肠癌中的表达及临床意义[J]. 第三军医大学学报, 2017,39(10):983-989.

Lai Wei, Zhe Wang, Houjie Liang. Expression of 6-phosphofructokinase-2 in colorectal cancer and its clinical significance[J]. Journal of Third Military Medical University, 2017, 39(10): 983-989.
魏莱, 王喆, 梁后杰. 6-磷酸果糖-2-激酶在结直肠癌中的表达及临床意义[J]. 第三军医大学学报, 2017,39(10):983-989. DOI： 10.16016/j.1000-5404.201612084.

Lai Wei, Zhe Wang, Houjie Liang. Expression of 6-phosphofructokinase-2 in colorectal cancer and its clinical significance[J]. Journal of Third Military Medical University, 2017, 39(10): 983-989. DOI： 10.16016/j.1000-5404.201612084.

摘要

目的

检测6-磷酸果糖-2-激酶(6-phosphofructokinase-2，PFKFB)在结直肠癌及癌旁组织中的表达，并探讨其临床意义。

方法

应用组织芯片结合免疫组化技术检测本院90例结直肠癌组织标本和相应90例癌旁组织中PFKFB1、PFKFB2、PFKFB3、PFKFB4及缺氧诱导因子-1(HIF1-α)的表达。

结果

PFKFB1、PFKFB2、PFKFB3、PFKFB4和HIF1-α在90例结直肠癌组织中高表达例数分别为57、59、59、51和60，表达均显著高于相应癌旁组织(

0.05)。PFKFB3在结直肠癌组织中的高表达与肿瘤的大小、临床分期、淋巴结转移及预后等密切相关(

0.05)，高表达组比低表达组预后差(

0.01)，临床分期高的结直肠癌患者中，PFKFB3的高表达提示了临床预后差(

0.01)，但在临床分期低的患者中不存在这种差异(

0.05)。PFKFB3的表达同HIF-1α的表达呈正相关(

=0.627

0.01)。而PFKFB1、PFKFB2和PFKFB4的表达与结直肠癌的肿瘤大小、临床分期、淋巴结转移及预后等无显著相关性(

0.05)。

结论

PFKFB家族在结直肠癌中高表达，且结直肠癌患者中PFKFB3高表达者比低表达者预后差，并且其作用同HIF1-α的表达密切相关。

Abstract

Objective

To investigate the expression level of 6-phosphofructokinase-2 (PFKFB) in human colorectal cancer and explore its clinical significance.

Methods

Tissue microarray combined with immunohistochemistry was used to detect the expression levels of PFKFB1

PFKFB2

PFKFB3

PFKFB4 and hypoxia-inducible factor-1(HIF1-α) in 90 colorectal cancer tissue samples and 90 paired adjacent tissue samples.

Results

High expression levels of PFKFB1

PFKFB2

PFKFB3

PFKFB4 and HIF1-α were detected in 57

51 and 60 of the colorectal cancer tissues

respectively

and their expression levels were significantly higher than in the paired adjacent tissues (

0.05). The expression level of PFKFB3 in the colorectal cancer tissue was closely correlated with the tumor size

clinical stage

lymph node metastasis and prognosis (

0.05). The patients with a high PFKFB3 expression level had poorer prognosis than those with a low PFKFB3 expression (

0.01). A high expression of PFKFB3 was

associated with a poor prognosis in the patients in an advanced stage (

0.01) but not in those in an early stage (

0.05). The expression level of PFKFB3 was positively correlated with HIF-1α (

=0.627

0.01). The expression levels of PFKFB1

PFKFB2 and PFKFB4 showed no obvious correlations with the tumor size

clinical stage

lymph node metastasis

or prognosis of the patients (

0.05).

Conclusion

The PFKFB family is highly expressed in colorectal cancers. A high expression of PFKFB3

which is positively correlated with HIF-1α expression

is associated with a poor prognosis of the patients

suggesting the potential role of PFKFB3 in tumorigenesis and progression of colorectal cancer.

关键词

Keywords

references

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