生姜中姜酚类活性成分的抗肿瘤作用及其机制

刘鑫; 张宏伟; 傅若秋; 高宁

doi:10.16016/j.1000-5404.201612059

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生姜中姜酚类活性成分的抗肿瘤作用及其机制

基础医学 | 更新时间：2026-01-09

- 生姜中姜酚类活性成分的抗肿瘤作用及其机制
- Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism
- 第三军医大学学报 2017年39卷第9期页码：884-890
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年科学基金(81202869)
- DOI：10.16016/j.1000-5404.201612059
  中图分类号：
- 收稿：2016-12-09，
  
  纸质出版：2017-05-15
- 稿件说明：
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刘鑫, 张宏伟, 傅若秋, 等. 生姜中姜酚类活性成分的抗肿瘤作用及其机制[J]. 第三军医大学学报, 2017,39(9):884-890.

Xin Liu, Hongwei Zhang, Ruoqiu Fu, et al. Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(9): 884-890.
刘鑫, 张宏伟, 傅若秋, 等. 生姜中姜酚类活性成分的抗肿瘤作用及其机制[J]. 第三军医大学学报, 2017,39(9):884-890. DOI： 10.16016/j.1000-5404.201612059.

Xin Liu, Hongwei Zhang, Ruoqiu Fu, et al. Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(9): 884-890. DOI： 10.16016/j.1000-5404.201612059.

摘要

Abstract

Objective

To extract

isolate and identify gingerols from ginger

determine their anti-cancer effect and investigate the underlying mechanism.

Methods

Supercritical CO

fluid extraction was used to extract ginger. The products were then isolated and purified by silica gel

RP-C

Sephadex LH-20 and high performance liquid chromatography (HPLC)

and identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The inhibitory effects of the obtained gingerols on cell proliferation in several cancer cell lines were evaluated by MTT assay. Flow cytometry and Western blotting were employed to determine the cell cycle and the expression of relevant proteins.

Results

There were 5 gingerols isolated from ginger

that is

4-gingerol

6-gingerol

8-gingerol

10-gingerol and 5′-hydroxy-6-gingerol. Among them

8-gingerol

and especially

10-gingerol exhibited potential inhibitory effects on the cell proliferation in 5 different kinds of cancer cells. The inhibitory concentrations of IC

were 25.80±1.39 and 35.29±2.70 μmol/L

respectively in the breast cancer MDA-MB-231 and MCF-7 cells after 72 hours' treatment with 10-gingerol. Flow cytometry found that 8-gingerol and 10-gingerol resulted in cell cycle arrest at G

phase

and the propor

tion of G

phase cells was (66.73±2.93)% and (66.59±2.49)%

respectively in the 2 breast cell lines after 10-gingerol treatment for 24 h

significantly higher than in the cells without treatment [(47.39±1.97)%

(49.17±3.52)%

0.01

]

. Western blotting indicated that 8-gingerol and 10-gingerol down-regulated the expression levels of G

phase related proteins

Cyclin D and CDK4 and decreased p-ERK and enhanced p-P38 in MAPK signaling pathway in MDA-MB-231 and MCF-7 cells.

Conclusion

8-gingerol and 10-gingerol derived from ginger exhibit obviously inhibitory effects on cancer cell proliferation

which are associated with its interruption of ERK and p38 MAPK signaling pathway and then leading to G

phase arrest.

关键词

Keywords

references

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