雌激素β受体对小鼠海马actin聚合调节蛋白表达的影响及其机制

何丽; 赵焱钢; 邱林利; 张媛媛; 赵继凯; 邢方舟; 张吉强; 李巍

doi:10.16016/j.1000-5404.201612048

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雌激素β受体对小鼠海马actin聚合调节蛋白表达的影响及其机制

基础医学 | 更新时间：2026-01-09

- 雌激素β受体对小鼠海马actin聚合调节蛋白表达的影响及其机制
- Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus
- 第三军医大学学报 2017年39卷第9期页码：852-858
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81571059)
- DOI：10.16016/j.1000-5404.201612048
  中图分类号：
- 收稿：2016-12-07，
  
  纸质出版：2017-05-15
- 稿件说明：
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何丽, 赵焱钢, 邱林利, 等. 雌激素β受体对小鼠海马actin聚合调节蛋白表达的影响及其机制[J]. 第三军医大学学报, 2017,39(9):852-858.

Li He, Yangang Zhao, Linli Qiu, et al. Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus[J]. Journal of Third Military Medical University, 2017, 39(9): 852-858.
何丽, 赵焱钢, 邱林利, 等. 雌激素β受体对小鼠海马actin聚合调节蛋白表达的影响及其机制[J]. 第三军医大学学报, 2017,39(9):852-858. DOI： 10.16016/j.1000-5404.201612048.

Li He, Yangang Zhao, Linli Qiu, et al. Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus[J]. Journal of Third Military Medical University, 2017, 39(9): 852-858. DOI： 10.16016/j.1000-5404.201612048.

摘要

Abstract

Objective

To detect the expression profile of estrogen receptor β (ERβ) in the postnatal hippocampus of male and female mice during development and investigate the underlying mechanism of ERβ activity on the regulation of actin polymerization related proteins.

Methods

C57BL/6 mice were divided into 10 groups by their sex and postnatal days (P0

P28 and P56). The expression of ERβ in the hippocampus was determined with immunohistochemical assay and Western blotting. Then adult female diestrus C57BL/6 mice were randomly divided into 5 groups

that is

control and PHTPP (ERβ inhibitor) treatment groups (1

5 and 7 d). DMSO and PHTPP (100 μg/kg) were intra-peritoneally injected. Then another 5 groups were treated respectively with DMSO

ovariectomy (OVX)

and DPN (ERβ activator

1.25

2.5 or 5.0 mg/kg) subcutaneously in 1 week after OVX. Immunohistochemical assay was used to examine the expression of steroid receptor coactivator-1 (SRC-1)

and Western blotting was employed to detect the expression of hippocampal Rictor

phospho-protein kinase B (p-Akt)

Profilin-1

phospho-cofilin ser3 (p-cofilin) and SRC-1.

Results

In both male and female hippocampus

high ERβ level was found at P0

then the level was decreased to the lower level at P7 and P14 (

0.05 compared with P0) and increased to the higher level at P28 and P56 (

0.01 compared with P7 and P14). In the adult mice after 7 days' PHTPP treatment

the expression levels of Rictor

p-Akt

Profilin-1

p-cofilin and SRC-1 were significantly decreased (

0.05). On the contrary

these levels was decreased dramatically after OVX but were significantly rescued by the 7 days' DPN treatment (

0.05).

Conclusion

Postnatal ERβ profile is in a U-shape trend in the hippocampus of mice. Manipulation of ERβ activity

Results

in the alternations of SRC-1 and actin cytoskeleton polymerization. This indicates ERβ may mediate estrogens regulation on learning and memory through SRC-1/Rictor/p-Akt pathway.

关键词

Keywords

references

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