照前给予去甲肾上腺素增强电离辐射对小鼠血小板生成能力的损伤作用

李凤菊; 杜长虹; 杨可; 雷鑫; 汤勇; 陈石磊; 许杨; 王军平

doi:10.16016/j.1000-5404.201612034

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照前给予去甲肾上腺素增强电离辐射对小鼠血小板生成能力的损伤作用

基础医学 | 更新时间：2026-01-09

- 照前给予去甲肾上腺素增强电离辐射对小鼠血小板生成能力的损伤作用
- Norepinephrine injection before ionizing radiation attenuates thrombopoiesis in mice
- 第三军医大学学报 2017年39卷第8期页码：724-730
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81573084);重庆市科技创新领军人才支持计划(CSTCKJCXLJRC06)
- DOI：10.16016/j.1000-5404.201612034
  中图分类号：
- 收稿：2016-12-06，
  
  纸质出版：2017-04-30
- 稿件说明：
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李凤菊, 杜长虹, 杨可, 等. 照前给予去甲肾上腺素增强电离辐射对小鼠血小板生成能力的损伤作用[J]. 第三军医大学学报, 2017,39(8):724-730.

Fengju Li, Changhong Du, Ke Yang, et al. Norepinephrine injection before ionizing radiation attenuates thrombopoiesis in mice[J]. Journal of Third Military Medical University, 2017, 39(8): 724-730.
李凤菊, 杜长虹, 杨可, 等. 照前给予去甲肾上腺素增强电离辐射对小鼠血小板生成能力的损伤作用[J]. 第三军医大学学报, 2017,39(8):724-730. DOI： 10.16016/j.1000-5404.201612034.

Fengju Li, Changhong Du, Ke Yang, et al. Norepinephrine injection before ionizing radiation attenuates thrombopoiesis in mice[J]. Journal of Third Military Medical University, 2017, 39(8): 724-730. DOI： 10.16016/j.1000-5404.201612034.

摘要

Abstract

Objective

To study the effect of norepinephrine (NE) on ionizing radiation (IR)-induced thrombocytopenia in mice and its possible mechanism.

Methods

Eighty normal C57BL/6 mice (8~10 weeks old) were randomly allocated into 4 equal groups and subjected to intraperitoneal injection of PBS or NE with or without total body irradiation (TBI) at 6 Gy for 24 h after the injection. At different time points following TBI

blood samples were collected from the tail vein of the mice for measuring platelet count and other routine blood biochemical parameters. The changes of the bone marrow cellularity were examined with HE staining at 9 d after TBI

and the changes in apoptosis of MO7e cells

cell cycle of megakaryocytic progenitors

and the frequency of LSK cells in mouse-derived Sca1

cells and in mice exposed to IR were analyzed with flow cytometry.

Results

As compared with those in PBS group

the platelet count in 3~10 d after injection and the frequency of HSCs of Lineage cells in murine bone marrow increased significantly [(1.44±0.08)%

(1.05±0.10)%

0.05

]

. In addition

NE treatment significantly promoted the proliferation of HSCs

in vitro

which showed a dose response relationship (≤100 nmol/mL concentration). Further more

HE staining showed that the numbers of bone marrow nucleated cells and megakaryocytes were significantly higher in NE group than those in PBS group. Moreover

(12.10±3.12)% of G

phase cells in the progenitor cells significantly decreased and (8.78±1.05)% of S phase cells in the progenitor cells significantly increased in NE group (

0.05). Effects were opposite when mice or cells were subje

cted to TBI after NE administration:the platelet count during the 7th~25th days and the number of bone marrow nucleated cells (

0.05)

the frequency of HSCs of Lineage-cells in murine bone marrow [(0.07±0.01)%

(0.27±0.03)%

0.05

]

and the number of bone marrow nucleated cells and megakaryocytes in NE+IR group were significantly lower than those in PBS+IR group. Besides

the apoptostic rate of megakaryocytic progenitors in NE+IR group was significantly higher than the rate of in PBS+IR group[ (84.46±5.09)%

(54.18±3.18)%

0.05

]

Conclusion

NE may aggravate acute radiation injury by unduly promoting proliferation of HSCs and megakaryocytic progenitors in mice.

关键词

Keywords

references

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