慢性缺氧诱导小鼠心肌细胞自噬及其机制

刘超; 蹇朝; 孟永胜; 彭文林; 朱昀; 姜云瀚; 罗桂平; 唐富琴; 肖颖彬

doi:10.16016/j.1000-5404.201611246

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慢性缺氧诱导小鼠心肌细胞自噬及其机制

基础医学 | 更新时间：2026-01-09

- 慢性缺氧诱导小鼠心肌细胞自噬及其机制
- Chronic hypoxia induces myocardial autophagy in mice
- 第三军医大学学报 2017年39卷第9期页码：859-864
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81270228)
- DOI：10.16016/j.1000-5404.201611246
  中图分类号：
- 收稿：2016-11-15，
  
  纸质出版：2017-05-15
- 稿件说明：
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刘超, 蹇朝, 孟永胜, 等. 慢性缺氧诱导小鼠心肌细胞自噬及其机制[J]. 第三军医大学学报, 2017,39(9):859-864.

Chao Liu, Zhao Jian, Yongsheng Meng, et al. Chronic hypoxia induces myocardial autophagy in mice[J]. Journal of Third Military Medical University, 2017, 39(9): 859-864.
刘超, 蹇朝, 孟永胜, 等. 慢性缺氧诱导小鼠心肌细胞自噬及其机制[J]. 第三军医大学学报, 2017,39(9):859-864. DOI： 10.16016/j.1000-5404.201611246.

Chao Liu, Zhao Jian, Yongsheng Meng, et al. Chronic hypoxia induces myocardial autophagy in mice[J]. Journal of Third Military Medical University, 2017, 39(9): 859-864. DOI： 10.16016/j.1000-5404.201611246.

摘要

Abstract

Objective

To determine the effect of chronic hypoxia on myocardial autophagy in mice and investigate the role of AMPKα2 knockout during the process.

Methods

Wild-type (WT) and AMPKα2 knockout (KO) male C57 mice were randomly divided into WT normoxic group

WT hypoxic group

KO normoxic group and KO hypoxic group

with 10 mice in each group. The normoxic groups were kept in normoxic condition

while the hypoxic groups were kept in a hypoxic chamber with 10% O

for 4 weeks. Then the blood samples of WT mice were collected for detecting the levels of red blood cells and hemoglobins. Heart samples of WT mice were harvested to detect the expression of AMPKα2 and p62 and ratio of autophagy-related protein LC3-Ⅱ/LC3-Ⅰ by Western blotting. The change of LC3 level was also detected by immunofluorescence staining in frozen sections. Cardiac samples of AMPKα2

-/-

mice were used to detect the LC3-Ⅱ/LC3-Ⅰ ratio by Western blotting.

Results

① In WT mice

the levels of red blood cells and hemoglobins in the hypoxic group were increased significantly than those in the normoxic group [(9.33±0.15)×10

(12.78±0.66)×10

0.05; 135±3

192±4 g/L

0.05

]

. ② In WT mice

the ratio of LC3-Ⅱ/LC3-Ⅰwas increased significantly (0.49±0.29

1.70±0.24

0.05)

the p62 expression level was decreased obviously

the relativ

e brightness of p62 was reduced (1.32±0.57

0.71±0.19

0.05)

and the fluorescence intensity of LC3 in the myocardium was increased in the hypoxic group than the normoxic group. ③ In normoxic conditions

the LC3-Ⅱ/LC3-Ⅰ ratio was lower in the myocardium

but not significantly (0.78±0.08

0.73±0.34

0.05) in AMPKα2

-/-

mice than the WT mice. While in hypoxic conditions

the ratio was significantly lower in the myocardium of AMPKα2

-/-

mice than the WT mice (2.08±0.72

1.01±0.21

0.05). ④ In WT mice

the expression level of AMPKα2 proteins was not significantly increased in the hypoxic group than the normoxic group (1.14±0.12

1.25±0.19

0.05).

Conclusion

Chronic hypoxia may enhance autophagy in cardiomyocytes by AMPKα2-dependent pathway

and the autophagy may be the adaptation mechanism of cardiomyocytes to chronic hypoxic conditions.

关键词

Keywords

references

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