宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系

朱宏斌; 沈伟; 王竞; 卢曙光; 赵岩; 乐率; 申梦宇; 李刚; 杨雨卉; 龚雅利; 李明; 谭银玲; 胡福泉

doi:10.16016/j.1000-5404.201611223

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宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系

基础医学 | 更新时间：2026-01-09

- 宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系
- Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study
- 第三军医大学学报 2017年39卷第8期页码：773-780
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201611223
  中图分类号：
- 纸质出版：2017-04
- 稿件说明：
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朱宏斌, 沈伟, 王竞, 等. 宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系[J]. 第三军医大学学报, 2017,39(8):773-780.

Hongbin Zhu, Wei Shen, Jing Wang, et al. Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study[J]. Journal of Third Military Medical University, 2017, 39(8): 773-780.
朱宏斌, 沈伟, 王竞, 等. 宏基因组研究高脂饮食诱导小鼠的肥胖易感性与肠道菌群的关系[J]. 第三军医大学学报, 2017,39(8):773-780. DOI： 10.16016/j.1000-5404.201611223.

Hongbin Zhu, Wei Shen, Jing Wang, et al. Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study[J]. Journal of Third Military Medical University, 2017, 39(8): 773-780. DOI： 10.16016/j.1000-5404.201611223.

摘要

Abstract

Objective

To investigate the correlation between gut microbiota and the susceptibility to high-fat diet-induced obesity in mice.

Methods

Stool samples were collected from C57BL/6J mice with high-fat diet-induced obesity (HFDIO)

mice with resistance to HFDIO (HFDIOR)

and normal control mice fed with normal diet. High-quality DNA samples extracted from the stool samples in the 3 groups (

=6) were taken for metagenomic sequencing using Hiseq2000 (Illumina Co. Ltd.).

Results

Compared with the control group

the mice with HFDIO and HFDIOR showed significantly increased

Firmicutes

Proteobacteria

and

Deferribacteres

while obviously decreased

Bacteroides

in the stool samples (

0.05). Significant differences were also found in the species of bacteria between the HFDIO and HFDIOR groups. The dominant species in the microbiota compositi

on was

Lachnospiraceae bacterium

10_1 in HFDIO group

and

Lachnospiraceae bacterium

28_4 was the dominant species in HFDIOR group (

0.05). The functionality and metabolism of the gut microbiota differed between the 2 model groups in terms of carbohydrate metabolism

nucleotide metabolism and nucleic acid binding

membrane transport and transferase activity.

Conclusion

The composition and function of the gut microbiota may influence the susceptibility of an individual to HFDIO and HFDIOR

which is helpful in assessing the role of gut microbiota in the occurrence of obesity.

关键词

Keywords

references

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