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去甲肾上腺素通过PI3K/Akt信号通路上调Sox4表达促进肝癌细胞增殖
Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression
via PI3K/Akt signal pathway- 第三军医大学学报 2017年39卷第9期 页码：821-826
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81272224)
DOI：10.16016/j.1000-5404.201611189
中图分类号：收稿：2016-11-22，
纸质出版：2017-05-15
- 稿件说明：
移动端阅览
杨大鹏, 宦洪波, 张亮, 等. 去甲肾上腺素通过PI3K/Akt信号通路上调Sox4表达促进肝癌细胞增殖[J]. 第三军医大学学报, 2017,39(9):821-826.
Dapeng Yang, Hongbo Huan, Liang Zhang, et al. Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression
via PI3K/Akt signal pathway[J]. Journal of Third Military Medical University, 2017, 39(9): 821-826.杨大鹏, 宦洪波, 张亮, 等. 去甲肾上腺素通过PI3K/Akt信号通路上调Sox4表达促进肝癌细胞增殖[J]. 第三军医大学学报, 2017,39(9):821-826. DOI： 10.16016/j.1000-5404.201611189.
Dapeng Yang, Hongbo Huan, Liang Zhang, et al. Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression
via PI3K/Akt signal pathway[J]. Journal of Third Military Medical University, 2017, 39(9): 821-826. DOI： 10.16016/j.1000-5404.201611189.
摘要
Abstract
Objective
2
To determine the effect of norepinephrine on self-renewal capacity in hepatocellular carcinoma (HCC) cells and investigate the molecular mechanism.
Methods
2
Quantitative RT-PCR and Western blotting were used to detect the expression level of CD133 in the HCC cell lines Huh7 and PLC after the treatment of 10 μmol/L norepinephrine. Sphere and clone formation assays were used to investigate the impact of norepinephrine on self-renewal capacity of HCC cells. The effects of norepinephrine (10 μmol/L) and prazosin (5 μmol/L) on the expression of stem related genes and signal pathways were detected by RT-PCR and Western blotting.
Results
2
Norepinephrine promoted the expression of CD133 in Huh7 and PLC cells. Norepinephrine facilitated sphere and clone formation in the 2 cell lines
and prazosin blocked the effect of norepinephrine (
P
<
0.05). There
were more sphere and clone formation in the cells after norepinephrine treatment than control cells and prazosin group (
P
<
0.05). Higher level of Sox4 and up-regulation of p-Akt were observed in the norepinephrine-treated cells than prazosin-treated and control HCC cells (
P
<
0.05).
Conclusion
2
Norepinephrine activates α-adrenergic receptor and up-regulates Sox4 expression
via
activating PI3K/Akt signal pathway
and thus improves self-renewal capacity in HCC cells.
关键词
Keywords
references
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