核苷酸结合寡聚化结构域样受体蛋白3炎性体在2型糖尿病并发冠心病过程中的作用

曹运兰; 胡龙江; 宋耀明

doi:10.16016/j.1000-5404.201611179

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核苷酸结合寡聚化结构域样受体蛋白3炎性体在2型糖尿病并发冠心病过程中的作用

临床医学 | 更新时间：2026-01-09

- 核苷酸结合寡聚化结构域样受体蛋白3炎性体在2型糖尿病并发冠心病过程中的作用
- Role of nucleotide binding oligomerization domain-like receptor protein 3 inflammasome in occurrence of coronary heart disease in patients with type 2 diabetes mellitus
- 第三军医大学学报 2017年39卷第8期页码：801-806
- 作者机构：
- 作者简介：
- 基金信息：
  
  重庆市卫生计生委医学科研资助面上项目(2015XMSB000312)
- DOI：10.16016/j.1000-5404.201611179
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- 纸质出版：2017-04
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曹运兰, 胡龙江, 宋耀明. 核苷酸结合寡聚化结构域样受体蛋白3炎性体在2型糖尿病并发冠心病过程中的作用[J]. 第三军医大学学报, 2017,39(8):801-806.

Yunlan Cao, Longjiang Hu, Yaoming Song. Role of nucleotide binding oligomerization domain-like receptor protein 3 inflammasome in occurrence of coronary heart disease in patients with type 2 diabetes mellitus[J]. Journal of Third Military Medical University, 2017, 39(8): 801-806.
曹运兰, 胡龙江, 宋耀明. 核苷酸结合寡聚化结构域样受体蛋白3炎性体在2型糖尿病并发冠心病过程中的作用[J]. 第三军医大学学报, 2017,39(8):801-806. DOI： 10.16016/j.1000-5404.201611179.

Yunlan Cao, Longjiang Hu, Yaoming Song. Role of nucleotide binding oligomerization domain-like receptor protein 3 inflammasome in occurrence of coronary heart disease in patients with type 2 diabetes mellitus[J]. Journal of Third Military Medical University, 2017, 39(8): 801-806. DOI： 10.16016/j.1000-5404.201611179.

摘要

Abstract

Objective

To investigate the role of inflammasome of nucleotide binding oligomerization domain-like receptor protein 3 (NLRP3) in the occurrence of coronary heart disease (CHD) in patients with type 2 diabetes mellitus (T2DM).

Methods

One hundred and thirty patients hospitalized in the Department of Cardiology

Xinqiao Hospital between March and August 2016 were enrolled in this study

including 40 with CHD

20 with T2DM group

and 40 with T2DM complicated by CHD

with 30 individuals without T2DM or CHD serving as the control group. Peripheral blood monocytes (HPBMCs) and plasma were collected from the subjects to detect the transcription and protein levels of NLRP3

apoptosis-associated speck-like protein (ASC) and caspase-1 using real-time qPCR and Western blotting; the plasma level of IL-1β was detected with enzyme-linked immunosorbent assay (ELISA).

Results

Both the mRNA and protein levels of NLRP3

ASC and caspase-1 in the HPBMCs were significantly increased in patients with CHD

T2DM and T2DM complicated by CHD groups as compared with the control group (

0.05). HPBMCs from diabetic patients with CHD patien

ts showed significantly higher ASC and caspase-1 mRNA levels and NLRP3 protein level than those from CHD or T2DM patients (

0.05). The mean plasma levels of IL-1β were 24.05±1.00

22.86±1.66

and 26.85±1.45 ng/L in CHD

T2DM and T2DM complicated by CHD groups

respectively

all significantly higher than the level in the control group (12.53±2.79 ng/L

0.05).

Conclusion

The aberrant activation of NLRP3 inflammasome promotes the occurrence of CHD in patients with T2DM and may serve as a new target for prevention and treatment of the CHD in diabetic patients.

关键词

Keywords

references

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