萋-尼染色导致的总挥发性有机物污染及其控制方法

金浩龙; 陈志谨; 熊昆; 金晓琳

doi:10.16016/j.1000-5404.201611159

您当前的位置：

首页 >

文章列表页 >

萋-尼染色导致的总挥发性有机物污染及其控制方法

临床医学 | 更新时间：2026-01-09

- 萋-尼染色导致的总挥发性有机物污染及其控制方法
- Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures
- 第三军医大学学报 2017年39卷第14期页码：1481-1485
- 作者机构：
  
  1.400037 重庆，第三军医大学新桥医院感染控制科
  2.400038 重庆，第三军医大学基础医学部微生物学教研室，重庆市微生物工程实验室
- 作者简介：
  
  [ 金晓琳, E-mail: jxl@tmmu.edu.cn Jin Xiaolin, E-mail: jxl@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(31570173)
- DOI：10.16016/j.1000-5404.201611159
  中图分类号： R136.32; R446
- 收稿：2016-11-18，
  
  修回：2017-02-23，
  
  纸质出版：2017-07-30
- 稿件说明：
移动端阅览
金浩龙, 陈志谨, 熊昆, 等. 萋-尼染色导致的总挥发性有机物污染及其控制方法[J]. 第三军医大学学报, 2017,39(14):1481-1485.

Haolong JIN, Zhijin CHEN, Kun XIONG, et al. Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures[J]. Journal of Third Military Medical University, 2017, 39(14): 1481-1485.
金浩龙, 陈志谨, 熊昆, 等. 萋-尼染色导致的总挥发性有机物污染及其控制方法[J]. 第三军医大学学报, 2017,39(14):1481-1485. DOI： 10.16016/j.1000-5404.201611159.

Haolong JIN, Zhijin CHEN, Kun XIONG, et al. Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures[J]. Journal of Third Military Medical University, 2017, 39(14): 1481-1485. DOI： 10.16016/j.1000-5404.201611159.

摘要

目的

建立一种改良抗酸染色冷染方法，用以控制实验室总挥发性有机物(total volatile organic compound，TVOC)污染。

方法

采用TVOC检测仪定量测定实验室内空气中的苯系物和TVOC污染程度；以卡介苗(bacillecalmette-guerin，BCG)为染色标本，配制含有1% Triton X-100标本稀释液和初染液，建立不用加温助染的抗酸染色冷染法替代传统抗酸染色法。

结果

传统萋-尼抗酸染色时，室内TVOC污染在12 min时间点即高达1 014 ppb(约1 mg/m

)，成倍超过国家室内卫生标准(GB50325-2010) 规定值(500 ppb

即0.5 mg/m

)；同样条件下，用改良冷染法染色期间，室内TVOC量有所增加，但在试验期间最高值为387 ppb

保持在国家室内卫生标准容许限度以下；利用Triton X-100建立的改良抗酸冷染法具有抗酸染色特性，其对抗酸杆菌的可检测度为3×10

-6

~6×10

-5

CFU/mL。

结论

抗酸染色冷染法可用于替代传统萋-尼抗酸染色，从而控制实验室的TVOC污染问题。

Abstract

Objective

To detect total volatile organic compound (TVOC) pollution in the laboratory during Ziehl-Neelsen acid-fast staining and establish an improved method for controlling TVOC pollution.

Methods

TVOC concentration in the room air of the laboratory was determined using RAE portable-type instrument during the performance of routine acid-fast staining of Bacille Calmette-Guerin (BCG). A dilution solution and a carbolfuchsin solution containing 1% Triton X-100 were prepared for use in the modified cold acid-fast staining without heating

and the TVOC pollution level caused by the modified method and the routine acid-fast staining method was compared.

Results

At 12 min after the start of routine acid-fast staining

the concentration of TVOC in the room air of the laboratory reached the highest level of 1 014 ppb (about 1 mg/m

)

which was 2 folds of the permissible exposure limit (500 ppb

0.5 mg/m

) in the National Standard (GB50325-2010). During the performance of the modified cold acid-fast staining

TVOC concentration in the laboratory increased only slightly and remained under the permissible exposure limit. The detection sensitivity of BCG ranged from 3×10

-6

to 6×10

-5

CFU using the modified cold staining method.

Conclusion

The modified acid-fast cold staining method can be used as an alternative to the conventional acid-fast staining for controlling TVOC pollution in the laboratory.

关键词

Keywords

references

徐志凯. 分枝杆菌属[M]//李凡, 徐志凯. 医学微生物学. 8版. 北京: 人民卫生出版社, 2013: 141-149. [https://ucdavis.pure.elsevier.com/en/organisations/medical-microbiology-and-immunology/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle .

XU Z K. Mycobacterium[M]//Li F, Xu Z K. Medical microbiology. The 8 edition.Bei Jing:People's Medical Publishing House, 2013: 141-149.

LAMANNA C. The nature of the acid-fast stain[J]. J Bacteriol, 1946, 52: 99-103.

VARUGHESE P, HELBECQUE D M, MCRAE K B, et al. Comparison of strip and Ziehl-Neelsen methods for staining acid-fast bacteria[J]. Bull World Health Organ, 1974, 51(1): 83-91.

ANGRA P, RIDDERHOF J, SMITHWICK R. Comparison of two different strengths of carbol fuchsin in Ziehl-Neelsen staining for detecting acid-fast bacilli[J]. J Clin Microbiol, 2003, 41(7): 3459. DOI: 10.1128/jcm.41.7.3459.2003.

CHEN P, SHI M, FENG G D, et al. A highly efficient Ziehl-Neelsen stain: identifying de novo intracellular Mycobacterium tuberculosis and improving detection of extracellular M. tuberculosis in cerebrospinal fluid[J]. J Clin Microbiol, 2012, 50(4): 1166-1170. DOI:10.1128/JCM.05756-11.

KURUP R, CHESTER K. Comparative evaluation of ziehl neelsen staining and knowledge,attitudes and practices of laboratory personnel in relation to ziehl nielsen[J]. West Indian Med J, 2014, 63(1): 34-39. DOI:10.7727/wimj.2012.247.

SELVAKUMAR N, RAHMAN F, RAJASEKARAN S, et al. Inefficiency of 0.3% carbol fuchsin in ziehl-neelsen staining for detecting acid-fast bacilli[J]. J Clin Microbiol, 2002, 40 (8): 3041-3043. DOI:10.1128/jcm.40.8.3041-3043.2002.

VASANTHAKUMARI R, JAGANNATH K, RAJASEKARAN S. A cold staining method for acid-fast bacilli[J]. Bull World Health Organ, 1986, 64(5): 741-743.

ZHAO D, YANG X M, CHEN Q Y, et al. A modified acid-fast staining method for rapid detection of Mycobacterium tuberculosis[J]. J Microbiol Methods, 2012, 91(1): 128-132. DOI: 10.1016/j.mimet.2012.07.024.

岳小琴.抗酸染色冷染法和加温染色法的应用分析[J].检验医学与临床, 2010, 7(23): 2651, 2658. DOI:10.3969/j.issn.1672-9455.2010.23.054.

YUE X Q. Acid fast stain cold staining and staining heating application analysis [J]. Laboratory Medicine and Clinic, 2010, 7(23): 2651, 2658. DOI: 10.3969/j.issn.1672-9455.2010.23.054.

张小芬, 刘平.抗酸染色冷染法和热染法查找痰中结核杆菌效果比较[J].实用预防医学, 2010, 17(10): 2101-2102. DOI: 10.3969/j.issn.1006-3110.2010.10.066.

ZHANG X F, LIU P. Comparative study of cold staining and heat staining for detection of acid-fast bacilli in sputum smears[J]. Practical Preventive Medicine, 2010, 17(10): 2101-2102. DOI: 10.3969/j.issn.1006-3110.2010.10.066.

邹毅实, 孙承阜, 方承德, 等.结核杆菌快速冷染法初步研究[J].中国防痨杂志, 1991, 13(4): 169-170.

ZOU Y S, SUN C G, FANG C D, et al. A preliminary study on rapid cold staining of Mycobacterium tuberculosis[J]. Chinese Journal of Antituberculosis, 1991, 13(4): 169-170.

饶贤才. 细菌分类与实验室安全[M]//汪正清. 医学微生物学. 北京: 人民卫生出版社, 2013: 84-100. [http://onlinelibrary.wiley.com/doi/10.1002/14651858.CD004559.pub4/references .

RAO X C. Bacterial classification and laboratory safety[M]//Wang Z Q.Medical microbiology. Bei Jing: People's Medical Publishing House, 2013: 84-100.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据