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早期腹腔穿刺引流对重症急性胰腺炎大鼠肠道细菌移位的影响
Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis
- 第三军医大学学报 2017年39卷第9期 页码：872-878
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金青年科学基金(81500409)
DOI：10.16016/j.1000-5404.201611157
中图分类号：收稿：2016-11-17，
纸质出版：2017-05-15
- 稿件说明：
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杨冠, 吴东叶, 肖和达, 等. 早期腹腔穿刺引流对重症急性胰腺炎大鼠肠道细菌移位的影响[J]. 第三军医大学学报, 2017,39(9):872-878.
Guan Yang, Dongye Wu, Heda Xiao, et al. Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis[J]. Journal of Third Military Medical University, 2017, 39(9): 872-878.
杨冠, 吴东叶, 肖和达, 等. 早期腹腔穿刺引流对重症急性胰腺炎大鼠肠道细菌移位的影响[J]. 第三军医大学学报, 2017,39(9):872-878. DOI： 10.16016/j.1000-5404.201611157.
Guan Yang, Dongye Wu, Heda Xiao, et al. Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis[J]. Journal of Third Military Medical University, 2017, 39(9): 872-878. DOI： 10.16016/j.1000-5404.201611157.
摘要
Abstract
Objective
2
To determine the effect of abdominal paracentesis drainage (APD) on the intestinal bacterial translocation in rats with severe acute pancreatitis (SAP).
Methods
2
The rats were intragastrically fed with
Escherichia coli
whose plasmids were reconstructed with green fluorescent protein (GFP-
E.coli
). These GFP-
E.coli
labeled rats were randomly divided into 3 groups: sham operation group (Sham group)
SAP group and APD group. The SAP rats were estab
lished by retrograde infusion of sodium taurocholate into biliopancreatic duct. The APD treatment was applied by inserting a drainage tube into the right lower quadrant of rats after SAP establishment. Spleen specimens were harvested in a sterile manner along with scarifying the rats in 24 h after operation. Bacterial translocation in the blood
mesenteric lymph nodes (MLNs) and pancreatic tissues
level of serum endotoxin
histological scores of pancreas
and serum levels of inflammatory factors CRP
TNF-α and IL-1β were assessed and compared among the 3 groups.
Results
2
Compared with sham operation group
the histological score of pancreas
bacterial translocation in the blood
MLNs and pancreatic tissues
serum endotoxin level
and serum levels of CRP
TNF-α and IL-1β were all significantly higher in the SAP group and APD group (
P
<
0.05). While compared with the SAP group
the above indicators were obviously lower in the APD group (
P
<
0.05).
Conclusion
2
Early APD reduces intestinal bacterial translocation and systemic inflammatory response in rats with SAP
and thus attenuates the severity of SAP.
关键词
Keywords
references
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