Tmub1沉默提高Akt蛋白活性促进肝细胞增殖

陈健; 付航玮; 刘孟刚; 陈景祥; 许建华; 王宇洲; 陈平

doi:10.16016/j.1000-5404.201611156

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Tmub1沉默提高Akt蛋白活性促进肝细胞增殖

基础医学 | 更新时间：2026-01-09

- Tmub1沉默提高Akt蛋白活性促进肝细胞增殖
- Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity
- 第三军医大学学报 2017年39卷第9期页码：827-832
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81270523);国家自然科学基金青年科学基金(81400651)
- DOI：10.16016/j.1000-5404.201611156
  中图分类号：
- 收稿：2016-11-17，
  
  纸质出版：2017-05-15
- 稿件说明：
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陈健, 付航玮, 刘孟刚, 等. Tmub1沉默提高Akt蛋白活性促进肝细胞增殖[J]. 第三军医大学学报, 2017,39(9):827-832.

Jian Chen, Hangwei Fu, Menggang Liu, et al. Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity[J]. Journal of Third Military Medical University, 2017, 39(9): 827-832.
陈健, 付航玮, 刘孟刚, 等. Tmub1沉默提高Akt蛋白活性促进肝细胞增殖[J]. 第三军医大学学报, 2017,39(9):827-832. DOI： 10.16016/j.1000-5404.201611156.

Jian Chen, Hangwei Fu, Menggang Liu, et al. Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity[J]. Journal of Third Military Medical University, 2017, 39(9): 827-832. DOI： 10.16016/j.1000-5404.201611156.

摘要

Abstract

Objective

To determine the effect of Tmub1 silencing on the phosphorylation of Akt and the proliferation and growth of hepatocytes.

Methods

RNA interference technique was used to knockdown Tmub1 gene in rat liver cell line BRL-3A. qRT-PCR and Western blotting were used to detect the expression of Tmub1

Akt

p-Akt

Cyclin D1 and c-myc at mRNA and protein levels. Plate colony formation assay and CCK-8 assay were employed to measure the proliferation and growth of BRL-3A cells. Flow cytometry was used to determine the cell cycle.

Results

Compared t

o the control group

the mRNA levels of Cyclin D1 and c-myc were up-regulated (

0.05)

the protein levels of p-Akt

Cyclin D1 and c-myc were increased (

0.05)

the proliferation and growth of BRL-3A cells were enhanced (

0.05)

and the percentage of cells at S+G

/M period was significantly increased (

0.05) in the cells after Tmub1 silencing. When the phosphorylation of Akt was inhibited by MK2206

the mRNA and protein levels of the genes mentioned above were significantly down-regulated

the proliferation ability of the BRL-3A cells was obviously reduced (

0.05)

and the percentage of cells at S+G

/M period was notably deceased (

0.05).

Conclusion

Tmub1 silencing promotes the proliferation and growth of BRL-3A cells by enhancing the phosphorylation of Akt and increasing the expression levels of Cyclin D1 and c-myc.

关键词

Keywords

references

付航玮陈健许建华 Tmub1对再生肝细胞有丝分裂的影响及其机制第三军医大学学报 2017 39 7 603 607 10.16016/j.1000-5404.201610134 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201610134 付航玮, 陈健, 许建华, 等. Tmub1对再生肝细胞有丝分裂的影响及其机制[J].第三军医大学学报, 2017, 39(7): 603-607.DOI:10.16016/j.1000-5404.201610134.

Fu H W Chen J Xu J H Effect of Tmub1 on mitosis of regenerating hepatocytes in rats J Third Mil Med Univ 2017 39 7 603 607 10.16016/j.1000-5404.201610134 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201610134 Fu H W, Chen J, Xu J H, et al. Effect of Tmub1 on mitosis of regenerating hepatocytes in rats[J].J Third Mil Med Univ, 2017, 39(7): 603-607.DOI:10.16016/j.1000-5404.201610134.

Jung Y Oh S H Witek R P Somatostatin stimulates the migration of hepatic oval cells in the injured rat liver Liver Int 2012 32 2 312 320 10.1111/j.1478-3231.2011.02642.x Jung Y, Oh S H, Witek R P, et al. Somatostatin stimulates the migration of hepatic oval cells in the injured rat liver[J]. Liver Int, 2012, 32(2): 312-320. DOI:10.1111/j.1478-3231.2011.02642.x.

Tordjmann T Hippo signalling: liver size regulation and beyond Clin Res Hepatol Gastroenterol 2011 35 5 344 346 10.1016/j.clinre.2011.01.012 Tordjmann T. Hippo signalling: liver size regulation and beyond[J]. Clin Res Hepatol Gastroenterol, 2011, 35(5): 344-346. DOI:10.1016/j.clinre.2011.01.012.

Yin S Wang H Park O Enhanced liver regeneration in IL-10-deficient mice after partial hepatectomy via stimulating inflammatory response and activating hepatocyte STAT3 Am J Pathol 2011 178 4 1614 1621 10.1016/j.ajpath.2011.01.001 Yin S, Wang H, Park O, et al. Enhanced liver regeneration in IL-10-deficient mice after partial hepatectomy via stimulating inflammatory response and activating hepatocyte STAT3[J ] . Am J Pathol, 2011, 178(4): 1614-1621. DOI:10.1016/j.ajpath.2011.01.001.

马德宾沈雁兵王保林 Tmub1沉默对大鼠肝部分切除术后肝细胞增殖的影响第三军医大学学报 2013 35 9 850 853 10.16016/j.1000-5404.2013.09.019 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201211089 马德宾, 沈雁兵, 王保林, 等. Tmub1沉默对大鼠肝部分切除术后肝细胞增殖的影响[J].第三军医大学学报, 2013, 35(9): 850-853. DOI: 10.16016/j.1000-5404.2013.09.019.

Ma D B Shen Y B Wang B L Influence of Tmub1 silencing on proliferation of rat hepatocytes after partial hepatectomy J Third Mil Med Univ 2013 35 9 850 853 10.16016/j.1000-5404.2013.09.019 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201211089 Ma D B, Shen Y B, Wang B L, et al. Influence of Tmub1 silencing on proliferation of rat hepatocytes after partial hepatectomy[J]. J Third Mil Med Univ, 2013, 35(9): 850-853.DOI: 10.16016/j.1000-5404.2013.09.019.

Livak K J Schmittgen T D Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method Methods 2001 25 4 402 408 10.1006/meth.2001.1262 Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C (T)) Method[J]. Methods, 2001, 25(4): 402-408. DOI:10.1006/meth.2001.1262.

Fujiyoshi M Ozaki M Molecular mechanisms of liver regeneration and protection for treatment of liver dysfunction and diseases J Hepatobiliary Pancreat Sci 2011 18 1 13 22 10.1007/s00534-010-0304-2 Fujiyoshi M, Ozaki M. Molecular mechanisms of liver regeneration and protection for treatment of liver dysfunction and diseases[J]. J Hepatobiliary Pancreat Sci, 2011, 18(1): 13-22. DOI:10.1007/s00534-010-0304-2.

赵晓彪李光耀刘孟刚肝部分切除术后Tmub1蛋白与securin蛋白的相互作用研究解放军医学杂志 2014 39 11 864 867 10.11855/j.issn.0577-7402.2014.11.04 赵晓彪, 李光耀, 刘孟刚, 等.肝部分切除术后Tmub1蛋白与securin蛋白的相互作用研究[J].解放军医学杂志, 2014, 39(11): 864-867. DOI:10.11855/j.issn. 0577-7402.2014.11.04.

Zhao X B Li G Y Liu M G Research on the interaction of protein Tmub1 and securin after partial hepatectomy Med J Chin PLA 2014 39 11 864 867 10.11855/j.issn.0577-7402.2014.11.04 Zhao X B, Li G Y, Liu M G, et al. Research on the interaction of protein Tmub1 and securin after partial hepatectomy[J]. Med J Chin PLA, 2014, 39(11):864-867. DOI: 10.11855/j.issn.0577-7402.2014.11.04.

赵晓彪李光耀刘孟刚 Tmub1过表达及沉默慢病毒载体转染对大鼠BRL-3A肝细胞增殖的影响解放军医学杂志 2016 41 1 12 16 10.11855/j.issn.0577-7402.2016.01.03 赵晓彪, 李光耀, 刘孟刚, 等. Tmub1过表达及沉默慢病毒载体转染对大鼠BRL-3A肝细胞增殖的影响[J].解放军医学杂志, 2016, 41(1): 12-16. DOI:10.11855/j.issn.0577-7402.2016.01.03.

Zhao X B Li G Y Liu M G Effects of over-expression and silenced lentivirus Tmub1 vectors transfection on the proliferation of rat liver cell line BRL-3A Med J Chin PLA 2016 41 1 12 16 10.11855/j.issn.0577-7402.2016.01.03 Zhao X B, Li G Y, Liu M G, et al. Effects of over-expression and silenced lentivirus Tmub1 vectors transfection on the proliferation of rat liver cell line BRL-3A[J]. Med J Chin PLA, 2016, 41(1):12-16.DOI: 10.11855/j.issn.0577-7402.2016.01.03.

Ilowski M Putz C Weiss T S Augmenter of liver regeneration causes different kinetics of ERK1/2 and Akt/PKB phosphorylation than EGF and induces hepatocyte proliferation in an EGF receptor independent and liver specific manner Biochem Biophys Res Commun 2010 394 4 915 920 10.1016/j.bbrc.2010.03.074 Ilowski M, Putz C, Weiss T S, et al. Augmenter of liver regeneration causes different kinetics of ERK1/2 and Akt/PKB phosphorylation than EGF and induces hepatocyte proliferation in an EGF receptor independent and liver specific manner[J]. Biochem Biophys Res Commun, 2010, 394(4): 915-920. DOI:10.1016/j.bbrc.2010.03.074.

Ng R Song G H Roll G R A microRNA-21 surge facilitates rapid cyclin D1 translation and cell cycle progression in mouse liver regeneration J Clin Invest 2012 122 3 1097 1108 10.1172/jci46039 Ng R, Song G H, Roll G R, et al. A microRNA-21 surge facilitates rapid cyclin D1 translation and cell cycle progression in mouse liver regeneration[J]. J Clin Invest, 2012, 122(3): 1097-1108. DOI:10.1172/jci46039.

Pauta M Rotllan N Fern ndez-Hernando A Akt-mediated foxo1 inhibition is required for liver regeneration Hepatology 2016 63 5 1660 1674 10.1002/hep.28286 Pauta M, Rotllan N, Fern ndez-Hernando A, et al. Akt-mediated foxo1 inhibition is required for liver regeneration[J]. Hepatology, 2016, 63(5): 1660-1674. DOI:10.1002/hep.28286.

Wen X Zhu J Dong L The role of c2orf68 and PI3K/Akt/mTOR pathway in human colorectal cancer Med Oncol 2014 31 8 92 10.1007/s12032-014-0092-7 Wen X, Zhu J, Dong L, et al. The role of c2orf68 and PI3K/Akt/mTOR pathway in human colorectal cancer[J]. Med Oncol, 2014, 31(8): 92. DOI:10.1007/s12032-014-0092-7.

Stemmann O Gorr I H Boos D Anaphase topsy-turvy: Cdk1 a securin, separase a CKI Cell Cycle 2006 5 1 11 13 10.4161/cc.5.1.2296 Stemmann O, Gorr I H, Boos D. Anaphase topsy-turvy: Cdk1 a securin, separase a CKI[J]. Cell Cycle, 2006, 5(1): 11-13. DOI:10.4161/cc.5.1.2296.

Liu M Liu H Wang X IL-6 induction of hepatocyte proliferation through the Tmub1-regulated gene pathway Int J Mol Med 2012 29 6 1106 1112 10.3892/ijmm.2012.939 Liu M, Liu H, Wang X, et al. IL-6 induction of hepatocyte proliferation through the Tmub1-regulated gene pathway[J]. Int J Mol Med, 2012, 29(6): 1106-1112. DOI:10.3892/ijmm.2012.939.

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