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LncRNA LOC100506123对胰腺癌细胞增殖及迁移的影响
Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells
- 第三军医大学学报 2017年39卷第9期 页码：840-845
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81372242);第三军医大学临床科研基金(SWH2015LC01)
DOI：10.16016/j.1000-5404.201611074
中图分类号：收稿：2016-11-09，
纸质出版：2017-05-15
- 稿件说明：
移动端阅览
赵志平, 李健, 王云超, 等. LncRNA LOC100506123对胰腺癌细胞增殖及迁移的影响[J]. 第三军医大学学报, 2017,39(9):840-845.
Zhiping Zhao, Jian Li, Yunchao Wang, et al. Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells[J]. Journal of Third Military Medical University, 2017, 39(9): 840-845.
赵志平, 李健, 王云超, 等. LncRNA LOC100506123对胰腺癌细胞增殖及迁移的影响[J]. 第三军医大学学报, 2017,39(9):840-845. DOI： 10.16016/j.1000-5404.201611074.
Zhiping Zhao, Jian Li, Yunchao Wang, et al. Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells[J]. Journal of Third Military Medical University, 2017, 39(9): 840-845. DOI： 10.16016/j.1000-5404.201611074.
摘要
Abstract
Objective
2
To determine the effect of long non-coding RNA (LncRNA) LOC100506123 on the proliferation and migration of pancreatic cancer cells.
Methods
2
Quantitative real-time PCR (qRT-PCR) was used to detect the expression of LOC100506123 in pancreatic cell lines
including normal pancreatic duct epithelial cell line HPDE and 4 pancreatic cancer cell lines
AsPC-1
BxPC-3
Panc-1 and CFPAC-1
and pancreatic cancer tissues. Small interfering RNA (siRNA) targeting LOC100506123 by RNA interference technique was constructed and then transfected into AsPC-1 and CFPAC-1 cells with Lipofectamine 2000 (including the control group siR-NC and the experimental groups siR-1 and siR-2). The proliferation and migration of pancreatic cancer cells were detected by Cell Counting Kit-8 (CCK-8) assay and Transwell chamber test
respectively.
Results
2
① LncRNA LOC100506123 was over-expressed in the pancreatic cancer cells and tissues (
P
<
0.05). ② Down-regulation of LOC100506123 significantly inhibited the proliferation and migration of pancreatic cancer cells (
P
<
0.05).
Conclusion
2
Over-expressed LOC100506123 promotes the proliferation and migration in pancreatic cancer cells.
关键词
Keywords
references
Abel E V Simeone D M Biology and clinical applications of pancreatic cancer stem cells Gastroenterology 2013 144 6 1241 1248 10.1053/j.gastro.2013.01.072 Abel E V, Simeone D M. Biology and clinical applications of pancreatic cancer stem cells[J]. Gastroenterology, 2013, 144(6): 1241-1248. DOI:10.1053/j.gastro.2013.01.072.
Chen W Zheng R Baade P D Cancer statistics in China, 2015 CA Cancer J Clin 2016 66 2 115 132 10.3322/caac.21338 Chen W, Zheng R, Baade P D, et al. Cancer statistics in China, 2015[J]. CA Cancer J Clin, 2016, 66(2): 115-132. DOI:10.3322/caac.21338.
Hijiya N Tsukamoto Y Nakada C Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma Cancer Res 2016 76 9 2612 2625 10.1158/0008-5472.CAN-15-1846 Hijiya N, Tsukamoto Y, Nakada C, et al. Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma[J]. Cancer Res, 2016, 76(9): 2612-2625. DOI:10.1158/0008-5472. CAN-15-1846.
Xu Y Chang R Peng Z Loss of polarity protein AF6 promotes pancreatic cancer metastasis by inducing Snail expression Nat Commun 2015 6 7184 10.1038/ncomms8184 Xu Y, Chang R, Peng Z, et al. Loss of polarity protein AF6 promotes pancreatic cancer metastasis by inducing Snail expression[J]. Nat Commun, 2015, 6: 7184. DOI:10.1038/ncomms8184.
Li Y Chen H Pan T LncRNA ontology: inferring lncRNA functions based on chromatin states and expression patterns Oncotarget 2015 6 37 39793 39805 10.18632/oncotarget.5794 Li Y, Chen H, Pan T, et al. LncRNA ontology: inferring lncRNA functions based on chromatin states and expression patterns[J]. Oncotarget, 2015, 6(37): 39793-39805. DOI:10.18632/oncotarget.5794.
Silva A Bullock M Calin G The Clinical Relevance of Long Non-Coding RNAs in Cancer Cancers 2015 7 4 2169 2182 10.3390/cancers7040884 Silva A, Bullock M, Calin G. The Clinical Relevance of Long Non-Coding RNAs in Cancer[J]. Cancers, 2015, 7(4): 2169-2182. DOI:10.3390/cancers7040884.
Li J Liu D Hua R Long non-coding RNAs expressed in pancreatic ductal adenocarcinoma and lncRNA BC008363 an independent prognostic factor in PDAC Pancreatology 2014 14 5 385 390 10.1016/j.pan.2014.07.013 Li J, Liu D, Hua R, et al. Long non-coding RNAs expressed in pancreatic ductal adenocarcinoma and lncRNA BC008363 an independent prognostic factor in PDAC[J]. Pancreatology, 2014, 14(5): 385-390. DOI:10.1016/j.pan.2014.07.013.
Di Gesualdo F Capaccioli S Lulli M A pathophysiological view of the long non-coding RNA world Oncotarget 2014 5 22 10976 10996 10.18632/oncotarget.2770 Di Gesualdo F, Capaccioli S, Lulli M. A pathophysiological view of the long non-coding RNA world[J]. Oncotarget, 2014, 5(22): 10976-10996. DOI:10.18632/oncotarget.2770.
He Y Meng X M Huang C Long noncoding RNAs: Novel insights into hepatocelluar carcinoma Cancer Lett 2014 344 1 20 27 10.1016/j.canlet.2013.10.021 He Y, Meng X M, Huang C, et al. Long noncoding RNAs: Novel insights into hepatocelluar carcinoma[J]. Cancer Lett, 2014, 344(1): 20-27. DOI:10.1016/j.canlet.2013.10.021.
Cheng Y Jutooru I Chadalapaka G The long non-coding RNA HOTTIP enhances pancreatic cancer cell proliferation, survival and migration Oncotarget 2015 6 13 10840 10852 10.18632/oncotarget.3450 Cheng Y, Jutooru I, Chadalapaka G, et al. The long non-coding RNA HOTTIP enhances pancreatic cancer cell proliferation, survival and migration[J]. Oncotarget, 2015, 6(13): 10840-10852. DOI:10.18632/oncotarget.3450.
Lu Z Xiao Z Liu F Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1) Oncotarget 2016 7 1 241 254 10.18632/oncotarget.6280 Lu Z, Xiao Z, Liu F, et al. Long non-coding RNA HULC promotes tumor angiogenesis in liver cancer by up-regulating sphingosine kinase 1 (SPHK1)[J]. Oncotarget, 2016, 7(1): 241-254. DOI:10.18632/oncotarget.6280.
Ji J Tang J Deng L LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway Oncotarget 2015 6 40 42813 42824 10.18632/oncotarget.5970 Ji J, Tang J, Deng L, et al. LINC00152 promotes proliferation in hepatocellular carcinoma by targeting EpCAM via the mTOR signaling pathway[J ] . Oncotarget, 2015, 6(40): 42813-42824. DOI:10.18632/ oncotarget.5970.
郭 勤浩 赵 岩 陈 洁静 BRAF激活的长链非编码RNA在结直肠癌中的表达及功能 中华消化外科杂志 2014 13 5 376 380 10.3760/cma.j.issn.1673-9752.2014.05.011 郭勤浩, 赵岩, 陈洁静, 等. BRAF激活的长链非编码RNA在结直肠癌中的表达及功能[J].中华消化外科杂志, 2014, 13(5): 376-380. DOI:10.3760/cma.j.issn. 1673-9752.2014.05.011.
Guo Q H Zhao Y Chen J J Expression and function of BRAF-activated long non-coding RNA in colorectal cancer Chin J Dig Surg 2014 13 5 376 380 10.3760/cma.j.issn.1673-9752.2014.05.011 Guo Q H, Zhao Y, Chen J J, et al. Expression and function of BRAF-activated long non-coding RNA in colorectal cancer[J]. Chin J Dig Surg, 2014, 13(5): 376-380. DOI:10.3760/cma.j.issn.1673-9752.2014.05.011.
Zhao L Guo H Zhou B Long non-coding RNA SNHG5 suppresses gastric cancer progression by trapping MTA2 in the cytosol Oncogene 2016 35 44 5770 5780 10.1038/onc.2016.110 Zhao L, Guo H, Zhou B, et al. Long non-coding RNA SNHG5 suppresses gastric cancer progression by trapping MTA2 in the cytosol[J]. Oncogene, 2016, 35(44): 5770-5780. DOI:10.1038/onc.2016.110.
Matsumura K Kawasaki Y Miyamoto M The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration Oncogene 2017 36 9 1191 1199 10.1038/onc.2016.282 Matsumura K, Kawasaki Y, Miyamoto M, et al. The novel G-quadruplex-containing long non-coding RNA GSEC antagonizes DHX36 and modulates colon cancer cell migration[J]. Oncogene, 2017, 36(9):1191-1199. DOI:10.1038/onc.2016.282.
Vennin C Spruyt N Robin Y M The long non-coding RNA 91H increases aggressive phenotype of breast cancer cells and up-regulates H19/IGF2 expression through epigenetic modifications Cancer Lett 2017 385 198 206 10.1016/j.canlet.2016.10.023 Vennin C, Spruyt N, Robin Y M, et al. The long non-coding RNA 91H increases aggressive phenotype of breast cancer cells and up-regulates H19/IGF2 expression through epigenetic modifications[J]. Cancer Lett, 2017, 385: 198-206. DOI:10.1016/j.canlet.2016.10.023.
吕 细林 黄 刚 肖 曼 过表达长链非编码RNA H19促进miR-124-3p表达抑制肝癌细胞增殖 第三军医大学学报 2016 38 6 554 559 10.16016/j.1000-5404.201508089 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201508089 吕细林, 黄刚, 肖曼, 等.过表达长链非编码RNA H19促进miR-124-3p表达抑制肝癌细胞增殖[J].第三军医大学学报, 2016, 38(6): 554-559. DOI:10.16016/j. 1000-5404.201508089.
Lyu X L Huang G Xiao M LncRNA H19-mediated miR-124-3p up-regulation suppresses proliferation in hepatocellular carcinoma cells J Third Mil Med Univ 2016 38 6 554 559 10.16016/j.1000-5404.201508089 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201508089 Lyu X L, Huang G, Xiao M, et al. LncRNA H19-mediated miR-124-3p up-regulation suppresses proliferation in hepatocellular carcinoma cells[J]. J Third Mil Med Univ, 2016, 38(6):554-559. DOI:10.16016/j.1000-5404.201508089.
Wang Y Li Z Zheng S Expression profile of long non-coding RNAs in pancreatic cancer and their clinical significance as biomarkers Oncotarget 2015 6 34 35684 35698 10.18632/oncotarget.5533 Wang Y, Li Z, Zheng S, et al. Expression profile of long non-coding RNAs in pancreatic cancer and their clinical significance as biomarkers[J]. Oncotarget, 2015, 6(34): 35684-35698. DOI:10.18632/oncotarget.5533.
Li X Deng S J Zhu S Hypoxia-induced lncRNA-NUTF2P3-001 contributes to tumorigenesis of pancreatic cancer by derepressing the miR-3923/KRAS pathway Oncotarget 2016 7 5 6000 6014 10.18632/oncotarget.6830 Li X, Deng S J, Zhu S, et al. Hypoxia-induced lncRNA-NUTF2P3-001 contributes to tumorigenesis of pancreatic cancer by derepressing the miR-3923/KRAS pathway[J]. Oncotarget, 2016, 7(5): 6000-6014. DOI:10.18632/oncotarget.6830.
Deer E L Gonz lez-Hern ndez J Coursen J D Phenotype and genotype of pancreatic cancer cell lines Pancreas 2010 39 4 425 435 10.1097/MPA.0b013e3181c15963 Deer E L, Gonz lez-Hern ndez J, Coursen J D, et al. Phenotype and genotype of pancreatic cancer cell lines[J]. Pancreas, 2010, 39(4): 425-435. DOI:10.1097/MPA. 0b013e3181c15963.
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