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细胞焦亡在新生儿坏死性小肠结肠炎中的致病作用
Pathogenic role of cell pyroptosis in necrotizing enterocolitis in neonatal rats
- 第三军医大学学报 2017年39卷第14期 页码：1440-1444
- 作者机构：
400014 重庆，重庆医科大学附属儿童医院新生儿科，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室，儿童发育重大疾病国家国际科技合作基地
- 作者简介：
[余加林, E-mail: yujialin486@126.com YU Jialin, E-mail: yujialin486@126.com
- 基金信息：国家自然科学基金(81370744);国家自然科学基金(81571483);国家自然科学基金(81601323);儿童医院临床研究项目((2014)254-lcyj2014-11)
DOI：10.16016/j.1000-5404.201611064
中图分类号： R329.25;R361;R725.746收稿：2016-11-08，
修回：2017-01-24，
纸质出版：2017-07-30
- 稿件说明：
移动端阅览
胡刘宏, 余加林, 艾青, 等. 细胞焦亡在新生儿坏死性小肠结肠炎中的致病作用[J]. 第三军医大学学报, 2017,39(14):1440-1444.
Liuhong HU, Jialin YU, Qing AI, et al. Pathogenic role of cell pyroptosis in necrotizing enterocolitis in neonatal rats[J]. Journal of Third Military Medical University, 2017, 39(14): 1440-1444.
胡刘宏, 余加林, 艾青, 等. 细胞焦亡在新生儿坏死性小肠结肠炎中的致病作用[J]. 第三军医大学学报, 2017,39(14):1440-1444. DOI： 10.16016/j.1000-5404.201611064.
Liuhong HU, Jialin YU, Qing AI, et al. Pathogenic role of cell pyroptosis in necrotizing enterocolitis in neonatal rats[J]. Journal of Third Military Medical University, 2017, 39(14): 1440-1444. DOI： 10.16016/j.1000-5404.201611064.
摘要
目的
2
探讨细胞焦亡是否参与新生儿坏死性小肠结肠炎(necrotizing enterocolitis，NEC)发病，并分析其可能的作用机制。
方法
2
将50只新生1 d的SD大鼠按随机数字表法分为2组(
n
=25)：正常对照组、坏死性小肠结肠炎新生大鼠组(NEC组)。正常对照组与母鼠同笼，不予处理；NEC组采用缺氧、冷刺激及人工喂养的方式建立模型。新生鼠于第1、2、3天固定时间点测量体质量，第4天处死大鼠，HE染色观察回盲部肠组织病理学变化并评分；qPCR检测NLRP3、IL-1β、IL-18基因表达水平；Western blot检测Caspase-1表达及激活情况；ELISA检测肠组织匀浆IL-1β、IL-18水平。
结果
2
与正常对照组相比，NEC组大鼠体质量明显降低，肠上皮损伤明显；NLRP3及IL-1β、IL-18基因表达增高(分别为0.33±0.06
vs
1.11±0.12，0.40±0.15
vs
1.25±0.13，1.04±0.16
vs
2.35±0.17，
P
＜0.05)；激活的Caspase-1仅在NEC组中表达，且下游炎症因子IL-1β及IL-18水平亦高于正常对照组(分别为300.4±76.5
vs
74.4±17.5，214.4±28.1
vs
23.9±19.3，
P
＜0.01)。
结论
2
细胞焦亡参与了NEC的发病，其具体机制可能与焦亡发生后IL-1β、IL-18的水平升高有关。
Abstract
Objective
2
To investigate whether cell pyroptosisis involved in the pathogenesis of neonatal necrotizing enterocolitis (NEC) and explore the possible mechanism.
Methods
2
Fifty neonatal (1-day-old) Sprague-Dawley rats were randomly divided into control group and NEC group (
n
=25). The rats in the control group were left with their mothers without particular treatment
and those in NEC group were subjected to gavage feeding
hypoxia and cold stress to establish models of NEC. The neonatal rats were weighed at a fixed time point of the day for 3 consecutive days. On day 4
all the rats were sacrificed by decapitation and a 2-cm segment of the proximal intestine at the ileocecal junction was harvested for histopathological evaluation. The mRNA expression levels of NLRP3
interleukin-1β (IL-1β)
and
IL-18 in the intestinal tissue were detected with quantitative real-time PCR
and the expression and activation level of caspase-1 were detected with Western blotting. Enzyme-linked immunosorbent assay was performed to detect the expression levels of IL-1β and IL-18 proteins in the intestine tissues.
Results
2
Compared with the control group
the neonatal rats in NEC group showed significantly lowered body weight and obvious intestinal injury. The intestinal tissues of the neonatal rats in NEC group showed significantly increased expressions of NLRP3
IL-1β and IL-18 at both the mRNA (
P
<
0.05) and protein (
P
<
0.01) levels as compared with those in the control group. Activated caspase-1 protein was detected only in NEC group.
Conclusion
2
Cell pyroptosis participates in the pathogenesis of neonatal intestinal injury
in which increased IL-1β and IL-18 expression following cell pyroptosis may play a role.
关键词
Keywords
references
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