泛素连接酶Parkin的表达与糖尿病肾病肾间质损伤的关系

袁俊杰; 陈客宏; 林利容; 郑卢权; 何娅妮

doi:10.16016/j.1000-5404.201611059

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泛素连接酶Parkin的表达与糖尿病肾病肾间质损伤的关系

基础医学 | 更新时间：2026-01-09

- 泛素连接酶Parkin的表达与糖尿病肾病肾间质损伤的关系
- Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy
- 第三军医大学学报 2017年39卷第4期页码：349-354
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81670661，81400733，81470962)
- DOI：10.16016/j.1000-5404.201611059
  中图分类号：
- 收稿：2016-11-07，
  
  纸质出版：2017-02-28
- 稿件说明：
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袁俊杰, 陈客宏, 林利容, 等. 泛素连接酶Parkin的表达与糖尿病肾病肾间质损伤的关系[J]. 第三军医大学学报, 2017,39(4):349-354.

Junjie Yuan, Kehong Chen, Lirong Lin, et al. Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy[J]. J Third Mil Med Univ, 2017, 39(4): 349-354.
袁俊杰, 陈客宏, 林利容, 等. 泛素连接酶Parkin的表达与糖尿病肾病肾间质损伤的关系[J]. 第三军医大学学报, 2017,39(4):349-354. DOI： 10.16016/j.1000-5404.201611059.

Junjie Yuan, Kehong Chen, Lirong Lin, et al. Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy[J]. J Third Mil Med Univ, 2017, 39(4): 349-354. DOI： 10.16016/j.1000-5404.201611059.

摘要

Abstract

Objective

To investigate the expression of Parkin in renal tissues of patients with diabetic nephropathy (DN) and the relationship between Parkin expression and renal tubulointerstitial injury.

Method

Totally 40 patients diagnosed with type 2 DN by renal biopsy admitted in our hospital between 2015 and 2016 were enrolled in this study. Ten patients with pathologically confirmed normal kidney tissues after renal tumor resection were taken as control group. Immunohistochemical examination were performed to detect the Parkin expression in renal tissues with different tubulointerstitial injury scored by interstitial fibrosis and tubular atrophy (IFTA) classification. The expression of Parkin correlating to clinical factors and renal pathological damage was determined by Pearson and Spearman correlation analyses

respectively. Immunofluorescence staining was performed to determine the expression of Parkin

renal interstitial injury factors e.g. interleukin (IL)-6

transforming growth factor beta (TGF-β)

and interstitial fibrosis marker collagen Ⅳ (Col-Ⅳ).

Results

Parkin was mainly expressed in the cytoplasm of renal tubular epithelial cells

and its expression level was gradually decreased with increased IFTA score. There was a significant negative correlation between Parki

n expression and renal tissue injury scores (glomerulosclerosis

tubular atrophy and interstitial fibrosis

renal interstitial inflammation) (

0.01). Such negative correlation was also found between Parkin expression and renal function injury parameters (quantitative urinary protein

urinary N-Acetyl-β-D glucosaminidase

blood urea nitrogen

serum cystatin C

serum creatinine)

while Parkin expression was positively correlated with estimated glomerular filtration rate (eGFR) (

0.05). Immunofluorescence assay showed that renal tubular epithelial cells with positive expression of Parkin did not co-express IL-6

TGF-β and Col-IV.

Conclusion

Decreased expression of Parkin is closely related to renal tubulointerstitial injury in patients with DN.

关键词

Keywords

references

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