LncRNA RNU12上调热休克蛋白72抑制重组人TRAIL蛋白诱导肝癌细胞凋亡

陈施翰; 邓青松; 蒋家云; 邹孟达; 谭运华; 马宽生

doi:10.16016/j.1000-5404.201610224

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LncRNA RNU12上调热休克蛋白72抑制重组人TRAIL蛋白诱导肝癌细胞凋亡

基础医学 | 更新时间：2026-01-09

- LncRNA RNU12上调热休克蛋白72抑制重组人TRAIL蛋白诱导肝癌细胞凋亡
- LncRNA RNU12 inhibits apoptosis of hepatocellular carcinoma cells induced by recombinant human TRAIL protein through up-regulating heat shock protein 72
- 第三军医大学学报 2017年39卷第9期页码：833-839
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81272688)
- DOI：10.16016/j.1000-5404.201610224
  中图分类号：
- 收稿：2016-10-31，
  
  纸质出版：2017-05-15
- 稿件说明：
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陈施翰, 邓青松, 蒋家云, 等. LncRNA RNU12上调热休克蛋白72抑制重组人TRAIL蛋白诱导肝癌细胞凋亡[J]. 第三军医大学学报, 2017,39(9):833-839.

Shihan Chen, Qingsong Deng, Jiayun Jiang, et al. LncRNA RNU12 inhibits apoptosis of hepatocellular carcinoma cells induced by recombinant human TRAIL protein through up-regulating heat shock protein 72[J]. Journal of Third Military Medical University, 2017, 39(9): 833-839.
陈施翰, 邓青松, 蒋家云, 等. LncRNA RNU12上调热休克蛋白72抑制重组人TRAIL蛋白诱导肝癌细胞凋亡[J]. 第三军医大学学报, 2017,39(9):833-839. DOI： 10.16016/j.1000-5404.201610224.

Shihan Chen, Qingsong Deng, Jiayun Jiang, et al. LncRNA RNU12 inhibits apoptosis of hepatocellular carcinoma cells induced by recombinant human TRAIL protein through up-regulating heat shock protein 72[J]. Journal of Third Military Medical University, 2017, 39(9): 833-839. DOI： 10.16016/j.1000-5404.201610224.

摘要

Abstract

Objective

To investigate the molecular mechanism of inhibiting the apoptosis of hepatocellular carcinoma (HCC) cells induced by recombinant human tumor necrosis factor-related apoptosis inducing ligand (TRAIL) protein at 46~50 ℃.

Methods

A sub-lethal heat treatment model of HCC cells was established. The Annexin-V/PI staining was used to detect the effect of treatment of recombinant human TRAIL on the apoptotic rate of HCC cells under different temperatures (43

46 and 50 ℃

and 37 ℃ as control temperature) for 10 min. According to th

e expression profile from the Agilent human lncRNA+mRNA Array V4.0 microarray data in HCC cells in our previous study

50 ℃ specific highly expressed LncRNA RNU12 was selected as the research object. Lentiviral vector LncRNA RNU12 was constructed

and then transfected into MHCC97-H cells. The expression of heat shock protein 72 (HSP72) at mRNA and protein levels were measured in different temperature treatment groups before and after transfection by qRT-PCR and Western blotting. Meanwhile

flow cytometry was used to detect the effect of lentiviral vector LncRNA RNU12 on the apoptosis of MHCC97-H cells induced by TRAIL protein. The fluorescence

in situ

hybridization (FISH) was used to detect the sub-cellular localization of LncRNA RNU12 in HCC cells.

Results

Temperature at 43 ℃ promoted recombinant human TRAIL protein to induce the apoptosis in HCC cells

but it exerted opposite effect at 46~50 ℃. The mRNA level of HSP72 was significantly decreased in all 4 treatment groups after lentiviral vector infection (

0.01). And TRAIL protein levels were obviously lower at the 37

46 and 50 ℃ groups than before lentivirus-mediated interference (

0.05). After the lentiviral vector infection

the apoptosis of MHCC97-H cells induced by TRAIL protein were increased by 7.36% (

0.01) and 8.84% (

0.01)

respectively at the 46℃ and 50℃ groups. RNA-FISH showed that LncRNA RNU12 was mainly expressed in the nucleus of MHCC97-H cells at 50℃.

Conclusion

LncRNA RNU12 up-regulating HSP72 at transcriptional level may be one of the molecular mechanisms of inhibiting the apoptosis of HCC cells induced by recombinant human TRAIL protein at 46~50℃.

关键词

Keywords

references

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