低氧和牙周炎对牙周组织中HIF-1α和MMP2表达的影响

徐倩; 杨佳佳; 刘志华; 刘锐; 安建平; 周霞; 陈发明; 邓蔓菁

doi:10.16016/j.1000-5404.201610220

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低氧和牙周炎对牙周组织中HIF-1α和MMP2表达的影响

更新时间：2026-01-09

- 低氧和牙周炎对牙周组织中HIF-1α和MMP2表达的影响
- Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium
- 第三军医大学学报 2017年39卷第7期页码：616-621
- 作者机构：
- 作者简介：
- 基金信息：
  
  军事口腔医学国家重点实验室开放课题项目(2014KA04)
- DOI：10.16016/j.1000-5404.201610220
  中图分类号：
- 收稿：2016-10-30，
  
  纸质出版：2017-07
- 稿件说明：
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徐倩, 杨佳佳, 刘志华, 等. 低氧和牙周炎对牙周组织中HIF-1α和MMP2表达的影响[J]. 第三军医大学学报, 2017,39(7):616-621.

Qian Xu, Jiajia Yang, Zhihua Liu, et al. Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium[J]. Journal of Third Military Medical University, 2017, 39(7): 616-621.
徐倩, 杨佳佳, 刘志华, 等. 低氧和牙周炎对牙周组织中HIF-1α和MMP2表达的影响[J]. 第三军医大学学报, 2017,39(7):616-621. DOI： 10.16016/j.1000-5404.201610220.

Qian Xu, Jiajia Yang, Zhihua Liu, et al. Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium[J]. Journal of Third Military Medical University, 2017, 39(7): 616-621. DOI： 10.16016/j.1000-5404.201610220.

摘要

Abstract

Objective

To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and matrix metalloproteinase 2 (MMP2) in the periodontal tissues of rats with periodontitis under hypoxia

and explore the relationship of HIF-1α and MMP2 expression with periodontitis.

Methods

Forty healthy adult rats were randomly divided into 4 groups: normoxia group

normoxic periodontitis group

hypoxia group (simulating 5 000 m above sea level) and hypoxic periodontitis group (

=10 for each group). Gingival index

plaque index

depth of probing and alveolar bone resorption were measured respectively. HE staining was used to observe periodontal inflammation in the periodontal tissue sections. Immunohistochemical assay and Western blotting were employed to detect the expression levels of HIF-1α and MMP2 in the periodontal tissues.

Results

Clinical indicators of periodontitis and the results of HE staining showed that the periodontal inflammation of the hypoxic periodontitis group was the most severe. Compared with the 2 normoxic groups

hypoxia promoted the expression of HIF-1α and MMP2 in the periodontal tissues in the 2 hypoxic groups (

0.05). The expression of HIF-1α and MMP2 was significantly higher in periodontitis groups than in the non-periodontitis groups (

0.05)

with the highest levels in the hypoxic periodontitis group (

0.05).

Conclusion

Hypoxia promotes the expression levels of HIF-1α and MMP2 in periodontitis and accelerates its progression.

关键词

Keywords

references

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