端粒酶逆转录酶线粒体转位促进肝癌细胞干性及耐药的研究

常杏; 胡长江; 邓盛瑜; 杨歆; 杨仕明; 凌贤龙

doi:10.16016/j.1000-5404.201610159

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端粒酶逆转录酶线粒体转位促进肝癌细胞干性及耐药的研究

基础医学 | 更新时间：2026-01-09

- 端粒酶逆转录酶线粒体转位促进肝癌细胞干性及耐药的研究
- Mitochondrial translocation of human telomerase reverse enzyme promotes stemness and drug-resistance of hepatocellular carcinoma cells
- 第三军医大学学报 2017年39卷第8期页码：781-786
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201610159
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- 纸质出版：2017-04
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常杏, 胡长江, 邓盛瑜, 等. 端粒酶逆转录酶线粒体转位促进肝癌细胞干性及耐药的研究[J]. 第三军医大学学报, 2017,39(8):781-786.

Xin Chang, Changjiang Hu, Shengyu Deng, et al. Mitochondrial translocation of human telomerase reverse enzyme promotes stemness and drug-resistance of hepatocellular carcinoma cells[J]. Journal of Third Military Medical University, 2017, 39(8): 781-786.
常杏, 胡长江, 邓盛瑜, 等. 端粒酶逆转录酶线粒体转位促进肝癌细胞干性及耐药的研究[J]. 第三军医大学学报, 2017,39(8):781-786. DOI： 10.16016/j.1000-5404.201610159.

Xin Chang, Changjiang Hu, Shengyu Deng, et al. Mitochondrial translocation of human telomerase reverse enzyme promotes stemness and drug-resistance of hepatocellular carcinoma cells[J]. Journal of Third Military Medical University, 2017, 39(8): 781-786. DOI： 10.16016/j.1000-5404.201610159.

摘要

Abstract

Objective

To determine the effect of mitochondrial translocation of human telomerase reverse enzyme (hTERT) on the stemness and drug-resistance of hepatocellular carcinoma (HCC) cells.

Methods

Human HCC cell lines HepG2 and Huh7 were exposed intermittently to cisplatin (CDDP) to obtain cisplatin-resistant cell lines HepG2/CDDP and Huh7/CDDP. CCK-8 assay was employed to detect cell sensitivity to CDDP. Western blotting was adopted to detect hTERT protein level in the mitochondria

and mitochondrial membrane potential was measured by confocal laser scanning microscopy. Flow cytometry was used to detect the expression of cell stemness-related proteins CD133 and EpCAM

and the colony formation ability of the cells was assessed with colony formation assay; Western blotting was used to detect the expression level of stemness-related factor OCT-4.

Results

The resistance indexes of CDDP-resistant HepG2 and Huh7 cells were significantly increased compared with their parental cells (HepG2: 41.16±0.42

7.69±0.86 μg/mL; Huh7: 33.48±0.33

7.18±0.35 μg/mL

0.01). The CDDP-resistant cells exhibited significantly increased mitochondrial hTERT expression and mitochondrial membrane potential with increased proportions of CD133

cells (HepG2: 1.44±0.41

HepG2/CDDP: 23.15±1.55

0.01) and EpCAM

cells (HepG2: 0.85±0.10

HepG2/CDDP: 3.96±0.10

0.01). The resistant cells also showed significantly enhanced colony formation ability and increased expression of OCT-4 protein.

Conclusion

The translocation of mitochondrial hTERT is significantly increased in CDDP-resistant cells

which possess obvious phenotypes of tumor stem cells.

关键词

Keywords

references

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