微小RNA-126基因在CD4<sup>+</sup> T细胞介导小鼠自身免疫性肠炎中的作用

褚风云; 胡燕; 郭萌萌; 陈超; 赵娟娟; 刘云; 秦娜琳; 郑静; 徐林

doi:10.16016/j.1000-5404.201610135

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微小RNA-126基因在CD4⁺ T细胞介导小鼠自身免疫性肠炎中的作用

基础医学 | 更新时间：2026-01-09

- 微小RNA-126基因在CD4⁺ T细胞介导小鼠自身免疫性肠炎中的作用
- Role of microRNA-126 in CD4⁺ T cell-mediated autoimmune colitis in mice
- 第三军医大学学报 2017年39卷第8期页码：767-772
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(31370918);贵州省高层次创新人才计划([黔科合人才 (2016)4031号]);遵义医学院优秀青年人才计划项目(15ZY-001)
- DOI：10.16016/j.1000-5404.201610135
  中图分类号：
- 纸质出版：2017-04
- 稿件说明：
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褚风云, 胡燕, 郭萌萌, 等. 微小RNA-126基因在CD4⁺ T细胞介导小鼠自身免疫性肠炎中的作用[J]. 第三军医大学学报, 2017,39(8):767-772.

Fengyun Chu, Yan Hu, Mengmeng Guo, et al. Role of microRNA-126 in CD4⁺ T cell-mediated autoimmune colitis in mice[J]. Journal of Third Military Medical University, 2017, 39(8): 767-772.
褚风云, 胡燕, 郭萌萌, 等. 微小RNA-126基因在CD4⁺ T细胞介导小鼠自身免疫性肠炎中的作用[J]. 第三军医大学学报, 2017,39(8):767-772. DOI： 10.16016/j.1000-5404.201610135.

Fengyun Chu, Yan Hu, Mengmeng Guo, et al. Role of microRNA-126 in CD4⁺ T cell-mediated autoimmune colitis in mice[J]. Journal of Third Military Medical University, 2017, 39(8): 767-772. DOI： 10.16016/j.1000-5404.201610135.

摘要

Abstract

Objective

To study the eff

ect of microRNA-126 knockdown on CD4

T cell-mediated autoimmune colitis in mice.

Methods

Wild-type mice and mice with microRNA-126 knockdown (miR-126KD) were fed with dextran sulfate sodium (DSS) solution for 7 d to induce acute autoimmune colitis. The pathological changes of the intestinal tissues were observed with HE staining

and the changes in CD4

T cell percentage and expression levels of CD62L and CD44 molecules in CD4

T cells were analyzed with fluorescence-activated cell sorting (FACS). CD4

CD62L

T cells purified from the spleen of miR-126 KD mice using magnetic activated cell sorting were stained with carboxyfluorescein diacetate

succinimidy1 ester (CFSE) and adoptively transferred into the wild-type mice

which were then exposed to DSS in drinking water to induce autoimmune colitis. The intestinal pathologies and changes of CD62L and CD44 expression in CFSE-positive cells of the recipient mice were analyzed with FACS.

Results

Compared with the normal control mice

miR-126KD mice with DSS-induced acute autoimmune colitis presented with obvious losses of intestinal villi

extensive ulcers in the mucosal epithelium and obvious infiltration of inflammatory cells in the intestinal tissues. FACS results showed that the percentage and total number of CD4

Tcells were increased significantly in miR-126KD mice with a high expression of CD44 and a low expression of CD62L in CD4

T cells (

0.01). Adoptive transfer experiments showed that miR-126KD CD4

T cell transfer aggravated intestinal tissue damage in wild-type mice with acute autoimmune colitis

and CFSE

cells from the wild-type mice showed an increased CD44 expression and a decreased CD62L expression.

Conclusion

miR-126 knockdown aggravates the pathological changes of CD4

Tcell-mediated inflammatory bowel disease in mice induc

ed by DSS.

关键词

Keywords

references

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微小RNA-126基因在CD4+ T细胞介导小鼠自身免疫性肠炎中的作用

Role of microRNA-126 in CD4+ T cell-mediated autoimmune colitis in mice

DOI：10.16016/j.1000-5404.201610135

摘要

Abstract

关键词

Keywords

references

微小RNA-126基因在CD4⁺ T细胞介导小鼠自身免疫性肠炎中的作用

Role of microRNA-126 in CD4⁺ T cell-mediated autoimmune colitis in mice