孕期炎症刺激对子代大鼠心肌纤维化的影响

温燕; 刘雅

doi:10.16016/j.1000-5404.201610118

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孕期炎症刺激对子代大鼠心肌纤维化的影响

基础医学 | 更新时间：2026-01-09

- 孕期炎症刺激对子代大鼠心肌纤维化的影响
- Maternal lipopolysaccharides exposure during pregnancy induces myocardiac fibrosis in offspring rats
- 第三军医大学学报 2017年39卷第8期页码：718-723
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81573440);国家自然科学基金面上项目(81373420)
- DOI：10.16016/j.1000-5404.201610118
  中图分类号：
- 收稿：2016-10-18，
  
  纸质出版：2017-04-30
- 稿件说明：
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温燕, 刘雅. 孕期炎症刺激对子代大鼠心肌纤维化的影响[J]. 第三军医大学学报, 2017,39(8):718-723.

Yan Wen, Ya Liu. Maternal lipopolysaccharides exposure during pregnancy induces myocardiac fibrosis in offspring rats[J]. Journal of Third Military Medical University, 2017, 39(8): 718-723.
温燕, 刘雅. 孕期炎症刺激对子代大鼠心肌纤维化的影响[J]. 第三军医大学学报, 2017,39(8):718-723. DOI： 10.16016/j.1000-5404.201610118.

Yan Wen, Ya Liu. Maternal lipopolysaccharides exposure during pregnancy induces myocardiac fibrosis in offspring rats[J]. Journal of Third Military Medical University, 2017, 39(8): 718-723. DOI： 10.16016/j.1000-5404.201610118.

摘要

Abstract

Objective

To explore the effect of maternal exposure to lipopolysaccharides (LPS) during pregnancy on myocardial fibrosis in offspring rats.

Methods

Eight pregnant rats were randomized into 2 equal groups to receive intraperitoneal injections of saline (control) or LPS (0.79 mg/kg) at the 8th

10th

and 12th days of gestation. The offspring rats were examined at 6

and 16 weeks after birth for systolic blood pressure using the tail-cuff method

and the ventricular histopathology was observed with Masson staining and transmission electron microscopy. The left ventricular myocardial tissues of the offspring rats were sampled for detecting the mRNA and protein expression levels of connexin (Cx) 43

microtubule-associated protein 1 light chain 3B (LC3B) and DNA methyltransferase 1 (DNMT1) using real-time PCR and Western blotting.

Results

Compared with those in the control group

the offspring rats in LPS group showed significantly increased systolic blood pressure at 6

and 16 weeks after birth (

0.01). No detectable pathological changes were observed in the offspring rats in the control group

whereas those with maternal LPS exposure presented with obvious myocardial pathologies in the left ventricle characterized by increased collagen deposition

disrupted myofibrils

decreased gap junctions

and an increased number of autophagosomes. The offspring rats with maternal LPS exposure showed significantly increased myocardial expression of Cx43 and DNMT-1 mRNA (

0.05) and decreased LC3B mRNA expression (

0.05) but with obviously decreased protein expression of Cx43 (

0.05) and increased expression of LC3BⅡ/LC3BⅠ and DNMT-1 proteins (

0.05).

Conclusion

Maternal LPS exposure during pregnancy can cause cardiac fibrosis in offspring rats possibly through a mechanism involving autophagy activation and Cx43 degradation.

关键词

Keywords

references

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