NF-κB抑制剂对脂多糖刺激的退变人椎间盘髓核细胞炎症因子表达的影响

易威威; 温亚枫; 刘浠; 叶禾; 刘渤

doi:10.16016/j.1000-5404.201610083

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NF-κB抑制剂对脂多糖刺激的退变人椎间盘髓核细胞炎症因子表达的影响

基础医学 | 更新时间：2026-01-09

- NF-κB抑制剂对脂多糖刺激的退变人椎间盘髓核细胞炎症因子表达的影响
- Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells
- 第三军医大学学报 2017年39卷第8期页码：755-759
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81572202)
- DOI：10.16016/j.1000-5404.201610083
  中图分类号：
- 收稿：2016-10-14，
  
  纸质出版：2017-04-30
- 稿件说明：
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易威威, 温亚枫, 刘浠, 等. NF-κB抑制剂对脂多糖刺激的退变人椎间盘髓核细胞炎症因子表达的影响[J]. 第三军医大学学报, 2017,39(8):755-759.

Weiwei Yi, Yafeng Wen, Xi Liu, et al. Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells[J]. Journal of Third Military Medical University, 2017, 39(8): 755-759.
易威威, 温亚枫, 刘浠, 等. NF-κB抑制剂对脂多糖刺激的退变人椎间盘髓核细胞炎症因子表达的影响[J]. 第三军医大学学报, 2017,39(8):755-759. DOI： 10.16016/j.1000-5404.201610083.

Weiwei Yi, Yafeng Wen, Xi Liu, et al. Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells[J]. Journal of Third Military Medical University, 2017, 39(8): 755-759. DOI： 10.16016/j.1000-5404.201610083.

摘要

Abstract

Objective

To investigate the changes in the expression of nuclear factor-κB (NF-κB) and inflammatory cytokines in degenerative human intervertebral disc nucleus pulposus cells in response to lipopolysaccharide (LPS) stimulation.

Methods

Cultured degenerative human nucleus pulposus cells were treated with PBS (control)

LPS (500 μg/mL)

or a NF-κB inhibitor (BAY11-7082) plus LPS (500 μg/mL). The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the culture medium were detected with enzyme-linked immunosorbent assay (ELISA). The expression and localization of p65 in the nucleus pulposus cells were detected with immunofluorescence assay

and the cellular expression of TNF-α

IL-1β

NF-κB-binding protein p65 and phosphorylated p65 (p-p65) were detected with Western blotting; Real-time PCR was used to detect the expression of TNF-α and IL-1β mRNA in the cells.

Results

LPS stimulation significantly increased the nuclear translocation of p65 and enhanced p-p65 expression in cultured nucleus pulposus cells. LPS stimulation of the nucleus pulposus cells caused sig

nificant increases in the levels of TNF-α and IL-1β both in the culture medium (

0.05) and in the cells (

0.05). Compared with LPS-exposed cells

the cells treated with BAY11-7082 plus LPS showed a significant decrease in p65 translocation and p-p65 expression with also decreased levels of TNF-α and IL-1β in both the culture medium (

0.05) and in the cells (

0.05).

Conclusion

LPS stimulation can promote the expression of TNF-α and IL-1β by activating NF-κB signaling pathway in human degenerative nucleus pulposus cells

and inhibition of NF-κB signaling pathway can significantly reduce the expression of these inflammatory factors.

关键词

Keywords

references

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