泌尿生殖道感染沙眼衣原体PmpF基因多态性及分布特征

常月立; 王威; 孙克; 张存辉; 张彦霞; 乔海霞; 张玉妥

doi:10.16016/j.1000-5404.201610080

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泌尿生殖道感染沙眼衣原体PmpF基因多态性及分布特征

更新时间：2026-01-09

- 泌尿生殖道感染沙眼衣原体PmpF基因多态性及分布特征
- Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens
- 第三军医大学学报 2017年39卷第7期页码：653-659
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北北方学院创新人才项目(CXRC1317)
- DOI：10.16016/j.1000-5404.201610080
  中图分类号：
- 收稿：2016-10-13，
  
  纸质出版：2017-07
- 稿件说明：
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常月立, 王威, 孙克, 等. 泌尿生殖道感染沙眼衣原体PmpF基因多态性及分布特征[J]. 第三军医大学学报, 2017,39(7):653-659.

Yueli Chang, Wei Wang, Ke Sun, et al. Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens[J]. Journal of Third Military Medical University, 2017, 39(7): 653-659.
常月立, 王威, 孙克, 等. 泌尿生殖道感染沙眼衣原体PmpF基因多态性及分布特征[J]. 第三军医大学学报, 2017,39(7):653-659. DOI： 10.16016/j.1000-5404.201610080.

Yueli Chang, Wei Wang, Ke Sun, et al. Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens[J]. Journal of Third Military Medical University, 2017, 39(7): 653-659. DOI： 10.16016/j.1000-5404.201610080.

摘要

Abstract

Objective

To investigate the polymorphism of polymorphic membrane protein F gene (PmpF) of

Chlamydia trachomatis

(

C.t

) causing urinogenital tract infection and its relationship with clinical infection.

Methods

Clinical urinogenital specimens were collected from No. 252 Hospital of PLA from January 2013 to December 2014.

C.t

nucleic acid detection kit was used to screen positive specimens. Then the total DNA of obtained strain was extracted

and PmpF gene was amplified with nested PCR. The product was digested with 4 restriction endonucleases

and its genotype was determined with restriction fragment length polymorphism (RFLP). According to the results of PFLP

the representative strains were selected to sequence its PmpF gene to draw phylogenetic trees. Omp1 gene of representative strains were amplified and sequenced

and the results were compared with the reference strains to determine serotypes of these strains.

Chi

-square test was used analyzed the relationship of PmpF genotype with gender and age

and its clinical distribution.

Results

There were 178

C.t

specimens obtained from the departments of urology

gynecology and dermatology during the 2 years. Based on the PmpF gene amplified from the 117 specimens

there were 3 genotypes

accounting for 2.6%

36.8% and 60.7%

respectively

according to results of RFLP. Twenty-six strains were selected to amplify and sequence Omp1 gene

and the results showed that serotypes of these strains were D

and J. Statistical analysis showed that the PmpF genotype was correlated with age (

Chi

-square=0.008)

but not with gender (

Chi

-square=0.167).

Conclusion

There are 3 genotypes

and 7 serotypes of

C.t

strains infecting urinogenital tract in our region. The serotypes have no corresponding relationship with PmpF genotype

and the genotypes are correlated with age

but not with gender.

关键词

Keywords

references

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