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SigB (Q225P) 突变促进金黄色葡萄球菌生物被膜形成
SigB (Q225P) mutation promotes formation of
Staphylococcus aureus biofilm- 第三军医大学学报 2017年39卷第8期 页码：711-717
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81672071)
DOI：10.16016/j.1000-5404.201610077
中图分类号：收稿：2016-10-13，
纸质出版：2017-04-30
- 稿件说明：
移动端阅览
刘慧, 尚伟龙, 彭华刚, 等. SigB (Q225P) 突变促进金黄色葡萄球菌生物被膜形成[J]. 第三军医大学学报, 2017,39(8):711-717.
Hui Liu, Weilong Shang, Huagang Peng, et al. SigB (Q225P) mutation promotes formation of
Staphylococcus aureus biofilm[J]. Journal of Third Military Medical University, 2017, 39(8): 711-717.刘慧, 尚伟龙, 彭华刚, 等. SigB (Q225P) 突变促进金黄色葡萄球菌生物被膜形成[J]. 第三军医大学学报, 2017,39(8):711-717. DOI： 10.16016/j.1000-5404.201610077.
Hui Liu, Weilong Shang, Huagang Peng, et al. SigB (Q225P) mutation promotes formation of
Staphylococcus aureus biofilm[J]. Journal of Third Military Medical University, 2017, 39(8): 711-717. DOI： 10.16016/j.1000-5404.201610077.
摘要
Abstract
Objective
2
To investigate the role of SigB (Q225P) in regulating biofilm formation of
Staphylococcus aureus
(
S
.
aureus
).
Methods
2
The biological characteristics of a clinically isolated
S
.
aureus
strain (XQ) and its mutant strain XQW obtained by passage were compared. The biofilm formation was assessed by crystal violet staining and laser confocal microscopy. Genome sequencing of the 2 strains was performed to compare the relevant genes and identify the mutation sites. Homologous recombination method was used to construct different mutant
S
.
aureus
strains
namely Newman-
sigB
(Q225P) with
sigB
(Q225P) gene replacement
Newman-△
sigB
with
sigB
deletion
and the complementary strains Newman-△
sigB
/
sigB
and Newman-△
sigB
/
sigB
(Q225P)
and biofilm formations of these strains were compared.
Results
2
XQW strain grew to form flocculent structures in liquid culture medium and showed a significantly greater ability of biofilm formation than its wild-type strain XQ as determined by crystal violet staining and laser confocal microscopy. Genome comparison suggested that a SigB (Q225P) mutation occurred in XQW strain. The constructed Newman-
sigB
(
Q225P) strain showed a much stronger ability of biofilm formation than wild-type Newman strain
a result consistent with the finding in XQW strain. The biofilm formation ability of Newman-△
sigB
/
sigB
(Q225P) strain was also stronger than that of Newman-△
sigB
/
sigB
strain.
Conclusion
2
SigB (Q225P) mutation promotes biofilm formation in
S
.
aureus
.
关键词
Keywords
references
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