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早期APP/PS1转基因AD模型小鼠海马胆碱能神经元改变的体视学研究
Stereological study of cholinergic neurons in hippocampus of early-stage APP/PS1 Alzheimer's disease transgenic mice
- 第三军医大学学报 2017年39卷第6期 页码：553-559
- 作者机构：
400016 重庆，重庆医科大学基础医学院人体解剖与组织胚胎学教研室，干细胞与组织工程研究室
- 作者简介：
[唐勇,E-mail:ytang062@163.comTang Yong, E-mail:ytang062@163.com
[吴宏,E-mail:wuhong6368@126.comWu Hong, E-mail:ytang062@163.com
- 基金信息：国家自然科学基金面上项目(81671259);国家自然科学基金面上项目(81470057);国家自然科学基金面上项目(81501101);重庆市首批“百名学术学科领军人才”培养计划(2012);重庆高校创新团队建设计划资助项目(2016)
DOI：10.16016/j.1000-5404.201610046
中图分类号： R-332;R322.81;R329.4收稿：2016-10-09，
修回：2016-10-27，
纸质出版：2017-06
- 稿件说明：
移动端阅览
李常琼, 晁凤蕾, 张蕾, 等. 早期APP/PS1转基因AD模型小鼠海马胆碱能神经元改变的体视学研究[J]. 第三军医大学学报, 2017,39(6):553-559.
Changqiong Li, Fenglei Chao, Lei Zhang, et al. Stereological study of cholinergic neurons in hippocampus of early-stage APP/PS1 Alzheimer's disease transgenic mice[J]. Journal of Third Military Medical University, 2017, 39(6): 553-559.
李常琼, 晁凤蕾, 张蕾, 等. 早期APP/PS1转基因AD模型小鼠海马胆碱能神经元改变的体视学研究[J]. 第三军医大学学报, 2017,39(6):553-559. DOI： 10.16016/j.1000-5404.201610046.
Changqiong Li, Fenglei Chao, Lei Zhang, et al. Stereological study of cholinergic neurons in hippocampus of early-stage APP/PS1 Alzheimer's disease transgenic mice[J]. Journal of Third Military Medical University, 2017, 39(6): 553-559. DOI： 10.16016/j.1000-5404.201610046.
摘要
目的
2
应用体视学方法定量研究APP/PS1双转基因AD模型小鼠海马内胆碱能神经元的早期改变情况。
方法
2
Morris水迷宫检测10月龄雌性APP/PS1双转基因AD小鼠 (APP/PS1组) 和同月龄同窝生雌性野生型小鼠 (Wild-type组) 的空间学习记忆能力。运用现代体视学方法结合免疫组织化学方法精确定量小鼠海马各亚区的体积和胆碱能神经元的数量。
结果
2
APP/PS1组小鼠定位航行实验中逃避潜伏期长于Wild-type组 (
P
<
0.01)。空间探索实验中，APP/PS1组小鼠穿越平台的次数及平台所在象限时间百分比均少于Wild-type组 (
P
=0.007、0.041)。APP/PS1组小鼠海马齿状回 (dentate gyrus，DG) 的体积较Wild-type组缩小 (
P
=0.001)，而APP/PS1组小鼠海马CA1、CA2/3区的体积较Wild-type组差异无统计学意义 (
P
=0.089、0.059)。APP/PS1组小鼠海马DG内胆碱能神经元的数量较Wild-type组显著减少 (
P
=0.017)，APP/PS1组小鼠海马CA1区、CA2/3区内胆碱能神经元较Wild-type组差异均无统计学意义 (
P
=0.076、0.074)。
结论
2
早期APP/PS1双转基因AD小鼠海马DG内胆碱能神经元丢失，可能是AD早期出现空间学习记忆能力障碍的重要结构基础之一。
Abstract
Objective
2
To investigate the early changes of cholinergic neurons in the hippocampus of APP/PS1 Alzheimer's disease (AD) transgenic mice.
Methods
2
Ten-month-old female APP/PS1 AD transgenic mice and age-matched littermate wild-type female mice were used. The spatial learning and memory abilities were tested with Morris water maze. The quantitative calculations for the volume of hippocampal sub-regions and the number of the cholinergic neurons in hippocampal sub-regions were obtained using the stereological methods.
Results
2
The escape latency time of the APP/PS1 transgenic group was significantly longer than that of the wild-type group (
P
<
0.01). There were significant differences in the times of the platform location crosses and the time spending in the target quadrant between the wild-type group and the APP/PS1 transgenic group (
P
=0.007
P
=0.041). The volume of the dentate gyrus and the number of the cholinergic neurons in the dentate gyrus were significantly redu
ced in the APP/PS1 transgenic group as compared with the wild-type group (
P
=0.001
P
=0.017). The volume of the CA1 and CA2/3 sectors and the number of the cholinergic neurons in the CA1 and CA2/3 sectors were not significantly changed in the APP/PS1 transgenic group as compared with the wild-type group (
P
=0.089
P
=0.059
P
=0.076
P
=0.074).
Conclusion
2
The loss of the cholinergic neurons in the dentate gyrus may be one of the morphological bases for the behavior deficits of the early-stage APP/PS1 AD transgenic mouse.
关键词
Keywords
references
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