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热射病小鼠脑组织小胶质细胞活化的变化
Changes of microglial activation after heat stroke in mice brain
- 第三军医大学学报 2017年39卷第4期 页码：317-322
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81472952);第三军医大学军事医学预研基金(2014XJY03)
DOI：10.16016/j.1000-5404.201610042
中图分类号：收稿：2016-10-09，
纸质出版：2017-02-28
- 稿件说明：
移动端阅览
王宫, 刘军, 何根林, 等. 热射病小鼠脑组织小胶质细胞活化的变化[J]. 第三军医大学学报, 2017,39(4):317-322.
Gong Wang, Jun Liu, Genlin He, et al. Changes of microglial activation after heat stroke in mice brain[J]. J Third Mil Med Univ, 2017, 39(4): 317-322.
王宫, 刘军, 何根林, 等. 热射病小鼠脑组织小胶质细胞活化的变化[J]. 第三军医大学学报, 2017,39(4):317-322. DOI： 10.16016/j.1000-5404.201610042.
Gong Wang, Jun Liu, Genlin He, et al. Changes of microglial activation after heat stroke in mice brain[J]. J Third Mil Med Univ, 2017, 39(4): 317-322. DOI： 10.16016/j.1000-5404.201610042.
摘要
Abstract
Objective
2
To explore the role of microglia played in mice brain after heat stroke by observing the effect of acute injury-related heat exposure on microglial activation.
Method
2
Fifty-seven adult male BALB/c mice were randomly divided into normal control group and 3 heat stroke groups after heat exposure of different time periods (1
6
and 24 h). The heat stroke groups were established by placing the mice in small animal environmental stimulation chambers with air temperature of 41.2±0.5 ℃ and relative humidity of （60±2）%.M1 polarization markers including TNF-α
CD45
and CD11b as well as M2 polarization markers including Arg1
FIZZ
and CD206 were measured using real-time reverse transcriptasc-polymerase chain reaction (RT-PCR). Protein expression of CD45
CD11b
FIZZ
and CD206 was detected by Western blotting. Distribution and co-localization of CD45 and CD206 were assessed by immunofluorescence staining.
Results
2
RT-PCR analysis showed that the mRNA levels of M1 polarization markers (TNF-α
CD45
and CD11b) and M2 polarization markers (Arg1
FIZZ
and CD206) reached the peak at 1 and 24 h after heat exposure(
P
<
0.01)
respectively. Western blot results revealed that the expression of M1 polarization markers CD45 and CD11b was significantly increased after 1 h followed by gradually declining to the base level 24 h after heat exposure. M2 polarization markers FIZZ and CD206 showed different expression trends
but both of their expression were significantly increased and reached the maximum at 24 h (
P
<
0.01). Immunofluorescence staining detected the co-localization of CD45 and CD206
exhibiting strong fluorescence signals at 1 and 24 h
respectively
after heat exposure in the cerebral cortex of mice.
Conclusion
2
Microglia are significantly activated in response to brain injury after heat exposure
which is characterized by prominent polarization of M1 at 1 h and M2 at 24 h after heat stroke. This polarized profile of microglia may be associated with central nervous system injury after heat stroke.
关键词
Keywords
references
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