热射病小鼠早期中枢神经炎症和外周炎症的变化

刘军; 王宫; 何根林; 杨举; 张晓亮; 罗珍; 申婷婷; 李萍; 李文晶; 杨学森

doi:10.16016/j.1000-5404.201609189

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热射病小鼠早期中枢神经炎症和外周炎症的变化

军事医学：热带医学 | 更新时间：2026-01-09

- 热射病小鼠早期中枢神经炎症和外周炎症的变化
- Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice
- 第三军医大学学报 2017年39卷第4期页码：311-316
- 作者机构：
- 作者简介：
- 基金信息：
  
  全军后勤科研“十二五”计划重点项目(BWS14J023);第三军医大学军事医学预研基金(2014XJY03)
- DOI：10.16016/j.1000-5404.201609189
  中图分类号：
- 收稿：2016-09-28，
  
  纸质出版：2017-02-28
- 稿件说明：
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刘军, 王宫, 何根林, 等. 热射病小鼠早期中枢神经炎症和外周炎症的变化[J]. 第三军医大学学报, 2017,39(4):311-316.

Jun Liu, Gong Wang, Genlin He, et al. Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice[J]. J Third Mil Med Univ, 2017, 39(4): 311-316.
刘军, 王宫, 何根林, 等. 热射病小鼠早期中枢神经炎症和外周炎症的变化[J]. 第三军医大学学报, 2017,39(4):311-316. DOI： 10.16016/j.1000-5404.201609189.

Jun Liu, Gong Wang, Genlin He, et al. Inflammatory responses of central and peripheral nerve system in early recovery stage of heat stroked mice[J]. J Third Mil Med Univ, 2017, 39(4): 311-316. DOI： 10.16016/j.1000-5404.201609189.

摘要

Abstract

Objective

To investigate the change and correlation of inflammatory response between the central and peripheral nerve system in early recovery stage (0~24 h)of mice after heat stroke(HS).

Method

A total of 70 BALB/c male mice were randomly divided into control (CON) group and other 5 different phase-group after HS (HS-0 h

HS-1 h

HS-6 h

HS-12 h

and HS-24 h). HS mouse model was established using environmental stimulation chamber. The survival

rectal temperature

and body weight loss were measured. The change of tumor necrosis factor alpha (TNF-α)

interleukin (IL)-1β

and IL-6 in the cortex and hippocampus was determined by quantitative real-time polymerase chain reaction(PCR). The serum levels of TNF-α

IL-1β

and IL-6 was measured by enzyme-linked immunosorbent assay (ELISA). The endotoxin concentration in the serum was detected by Kinetic Turbidimetric Tachypleus Amebocyte Lysate.

Results

The rectal temperature in mice exceeded 42 ℃ after heat exposure.Hypothermia occurred in 1~2 h and the survival rate of mice was 78.57%after 24 h. These suggested that HS mouse model was successfully generated. Compared to the control group

the mRNA expression of TNF-α

IL-1β

and IL-6 in the cerebral cortex and hippocampus of HS groups was increased rapidly

reaching the maximum at 1 h (

0.01). The expression was then gradually decreased after 6 h and approached to the normal level at 24 h (

0.05).At 6 h

the expression of these inflammatory factors was still higher than the CON group (

0.01).The expression levels of TNF-α

IL-1β

and

IL-6 in the peripheral serum were increased significantly at 1 h

then decreased. At 24 h

the levels still remained higher than the CON group (

0.01). The concentration of endotoxin in the serum was elevated at 0 h

reached the peak at 6 h and maintained the high secretion level until 24 h (

0.01).

Conclusion

The secretion of inflammatory factors in central nerve system response to heat exposure is transient within 12 h

while is persistent to 24 h in peripheral inflammatory secretion.The different inflammation response between the central and peripheral nerve system after heat stroke implies that they are independent of each other in early stage of recovery in mice.

关键词

Keywords

references

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易均凤何根林杨学森热习服对热射病小鼠中枢神经细胞自噬及凋亡的影响第三军医大学学报 2015 37 11 1059 1063 10.16016/j.1000-5404.201502011 易均凤, 何根林, 杨学森, 等. 热习服对热射病小鼠中枢神经细胞自噬及凋亡的影响[J]. 第三军医大学学报,2015,37(11): 1059-1063. DOI: 10.16016/j.1000-5404.201502011

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