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骨母特异性因子2促进瘢痕疙瘩间充质干细胞体外增殖的研究
Effect of periostin on proliferation of keloid mesenchymal stem cells
in vitro - 第三军医大学学报 2017年39卷第3期 页码：236-242
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81272102)
DOI：10.16016/j.1000-5404.201609102
中图分类号：收稿：2016-09-14，
纸质出版：2017-02-15
- 稿件说明：
移动端阅览
王雪丽, 马昕, 李乐, 等. 骨母特异性因子2促进瘢痕疙瘩间充质干细胞体外增殖的研究[J]. 第三军医大学学报, 2017,39(3):236-242.
Xueli Wang, Xin Ma, Le Li, et al. Effect of periostin on proliferation of keloid mesenchymal stem cells
in vitro [J]. Journal of Third Military Medical University, 2017, 39(3): 236-242.王雪丽, 马昕, 李乐, 等. 骨母特异性因子2促进瘢痕疙瘩间充质干细胞体外增殖的研究[J]. 第三军医大学学报, 2017,39(3):236-242. DOI： 10.16016/j.1000-5404.201609102.
Xueli Wang, Xin Ma, Le Li, et al. Effect of periostin on proliferation of keloid mesenchymal stem cells
in vitro [J]. Journal of Third Military Medical University, 2017, 39(3): 236-242. DOI： 10.16016/j.1000-5404.201609102.
摘要
Abstract
Objective
2
To determine the effect of periostin (also know as osteoblast-specific factor 2) on the proliferation of keloid mesenchymal stem cells (KMLSCs)
in vitro
.
Methods
2
KMLSCs and skin precursors (SKPs) were isolated from keloid tissues and the corresponding skin tissues respectively
and then cultured. The third passage cells were sorted by flow cytometry and then expanded. The expression of periostin was detected by immunocytochemical assay and Western blotting. The shPeriostin lentiviral vector was constructed
and then infected into KMLSCs. The interference efficiency was determined by qPCR and Western blotting. Cell proliferation
apoptosis and cell cycle in the KMLSCs after shPeriostin or blank vector infection and untreated cells were detected by MTT assay and flow cytometry. The expression of LRP5 was determined by immunofluorescent staining and Western blotting.
Results
2
The KMLSCs and SKPs were isolated from keloid and corresponding skin tissues
with
a
proportion of 94%. Immunocytochemical assay and Western blot analysis showed that the expression level of periostin was significantly higher in KMLSCs than in SKPs (
P
<
0.05). qPCR and Western blotting indicated that the expression of periostin was inhibited after the infection of shPeriostin lentiviral vector. Knockdown of periostin resulted in inhibitory proliferation
enhanced apoptosis and more cells arrested at G
1
phase in KMLSCs. Knockdown of periostin also led to the decreased expression of LRP5 (
P
<
0.05).
Conclusion
2
Knockdown of periostin induces apoptosis
inhibits cell cycle transition
and decreases proliferation in KMLSCs
which might be due to its effect on the expression of LRP5.
关键词
Keywords
references
Takeshita S Kikuno R Tezuka K Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin Ⅰ Biochem J 1993 294 Pt 1 271 278 https://www.researchgate.net/publication/14834885_Osteoblast-specific_factor_2_Cloning_of_a_putative_bone_adhesion_protein_with_homology_with_the_insect_protein_fasciclin_I Takeshita S, Kikuno R, Tezuka K, et al. Osteoblast-specific factor 2: cloning of a putative bone adhesion protein with homology with the insect protein fasciclin Ⅰ[J]. Biochem J, 1993, 294 (Pt 1): 271-278.
Wu S Q Lv Y E Lin B H Silencing of periostin inhibits nicotine-mediated tumor cell growth and epithelial-mesenchymal transition in lung cancer cells Mol Med Rep 2013 7 3 875 880 10.3892/mmr.2013.1267 Wu S Q, Lv Y E, Lin B H, et al. Silencing of periostin inhibits nicotine-mediated tumor cell growth and epithelial-mesenchymal transition in lung cancer cells[J]. Mol Med Rep, 2013, 7(3): 875-880. DOI: 10.3892/mmr.2013.1267
Zhang Z Nie F Kang C Increased periostin expression affects the proliferation, collagen synthesis, migration and invasion of keloid fibroblasts under hypoxic conditions Int J Mol Med 2014 34 1 253 261 10.3892/ijmm.2014.1760 Zhang Z, Nie F, Kang C, et al. Increased periostin expression affects the proliferation, collagen synthesis, migration and invasion of keloid fibroblasts under hypoxic conditions[J]. Int J Mol Med, 2014, 34(1): 253-261. DOI: 10.3892/ijmm.2014.1760
Crawford J Nygard K Gan B S Periostin induces fibroblast proliferation and myofibroblast persistence in hypertrophic scarring Exp Dermatol 2015 24 2 120 126 10.1111/exd.12601 Crawford J, Nygard K, Gan B S, et al.Periostin induces fibroblast proliferation and myofibroblast persistence in hypertrophic scarring[J]. Exp Dermatol, 2015, 24(2): 120-126. DOI: 10.1111/exd.12601
Li Y Yang X Nie F F Biological characteristics of human adipose-derived stem cells and their response to periostin in vitro Chin Med J (Engl) 2013 126 8 1491 1497 https://www.researchgate.net/publication/236224684_Biological_characteristics_of_human_adipose-derived_stem_cells_and_their_response_to_periostin_in_vitro Li Y, Yang X, Nie F F, et al. Biological characteristics of human adipose-derived stem cells and their response to periostin in vitro[J]. Chin Med J (Engl), 2013, 126(8): 1491-1497.
Qu Y Chi W Hua X Unique Expression Pattern and Functional Role of Periostin in Human Limbal Stem Cells PLoS One 2015 10 2 e0117139 10.1371/journal.pone.0117139 Qu Y, Chi W, Hua X, et al. Unique Expression Pattern and Functional Role of Periostin in Human Limbal Stem Cells[J]. PLoS One, 2015, 10(2): e0117139. DOI: 10.1371/journal.pone.0117139
Ma S M Chen L X Lin Y F Periostin Promotes Neural Stem Cell Proliferation and Differentiation following Hypoxic-Ischemic Injury PLoS One 2015 10 4 e0123585 10.1371/journal.pone.0123585 Ma S M, Chen L X, Lin Y F, et al. Periostin Promotes Neural Stem Cell Proliferation and Differentiation following Hypoxic-Ischemic Injury[J]. PLoS One, 2015, 10(4): e0123585. DOI: 10.1371/journal.pone.0123585
Moon J H Kwak S S Park G Isolation and Characterization of Multipotent Human Keloid-Derived Mesenchymal-Like Stem Cells Stem Cells Dev 2008 17 4 713 724 10.1089/scd.2007.0210 Moon J H, Kwak S S, Park G, et al.Isolation and Characterization of Multipotent Human Keloid-Derived Mesenchymal-Like Stem Cells[J]. Stem Cells Dev, 2008, 17(4): 713-724. DOI: 10.1089/scd.2007.0210
Zhang Q Yamaza T Kelly A P Tumor-like stem cells derived from human keloid are governed by the inflammatory niche driven by IL-17/IL-6 axis PLoS One 2009 4 11 e7798 10.1371/journal.pone.0007798 Zhang Q, Yamaza T, Kelly A P, et al. Tumor-like stem cells derived from human keloid are governed by the inflammatory niche driven by IL-17/IL-6 axis[J]. PLoS One, 2009, 4(11): e7798. DOI: 10.1371/journal.pone.0007798
Qu M Song N Chai G Pathological niche environment transforms dermal stem cells to keloid stem cells: A hypothesis of keloid formation and development Med Hypotheses 2013 81 5 807 812 10.1016/j.mehy.2013.08.033 Qu M, Song N, Chai G, et al. Pathological niche environment transforms dermal stem cells to keloid stem cells: A hypothesis of keloid formation and development[J]. Med Hypotheses, 2013, 81(5): 807-812. DOI: 10.1016/j.mehy.2013.08.033
Li B Wang L Chi B Upregulation of periostin prevents P53-mediated apoptosis in SGC-7901 gastric cancer cells Mol Biol Rep 2013 40 2 1677 1683 10.1007/s11033-012-2218-3 Li B, Wang L, Chi B. Upregulation of periostin prevents P53-mediated apoptosis in SGC-7901 gastric cancer cells[J]. Mol Biol Rep, 2013, 40(2): 1677-1683. DOI: 10.1007/s11033-012-2218-3
Kühn B del Monte F Hajjar R J Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair Nat Med 2007 13 8 962 969 10.1038/nm1619 Kühn B, del Monte F, Hajjar R J, et al. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair[J]. Nat Med, 2007, 13(8): 962-969. DOI: 10.1038/nm1619
Iqbal S A Sidgwick G P Bayat A Identification of fibrocytes from mesenchymal stem cells in keloid tissue: a potential source of abnormal fibroblasts in keloid scarring Arch Dermatol Res 2012 304 8 665 671 10.1007/s00403-012-1225-5 Iqbal S A, Sidgwick G P, Bayat A, et al. Identification of fibrocytes from mesenchymal stem cells in keloid tissue: a potential source of abnormal fibroblasts in keloid scarring[J]. Arch Dermatol Res, 2012, 304(8): 665-671. DOI: 10.1007/s00403-012-1225-5
王 珍祥 袁 顺宗 陶 熹 SFRP 2与Periostin的相互作用在瘢痕疙瘩形成机制中的研究 组织工程与重建外科杂志 2009 5 4 199 201 10.3969/j.issn.1673-0364.2009.08.005 王珍祥, 袁顺宗, 陶熹, 等. SFRP 2与Periostin的相互作用在瘢痕疙瘩形成机制中的研究[J].组织工程与重建外科杂志, 2009, 5(4): 199-201, 207. DOI: 10.3969/j.issn.1673-0364.2009.08.005
Lambert A W Wong C K Ozturk S Tumor Cell-Derived Periostin Regulates Cytokines That Maintain Breast Cancer Stem Cells Mol Cancer Res 2016 14 1 103 113 10.1158/1541-7786.MCR-15-0079 Lambert A W, Wong C K, Ozturk S, et al. Tumor Cell-Derived Periostin Regulates Cytokines That Maintain Breast Cancer Stem Cells[J]. Mol Cancer Res, 2016, 14(1): 103-113. DOI: 10.1158/1541-7786.MCR-15-0079
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