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下调Cyclophilin A对喉鳞癌裸鼠移植瘤模型肿瘤生长和转移的影响
Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice
- 第三军医大学学报 2017年39卷第4期 页码：355-360
- 作者机构：
- 作者简介：
- 基金信息：国家临床重点专科建设项目(卫办医政函[2012]649号);重庆市卫生局医学科研重点项目(20121024)
DOI：10.16016/j.1000-5404.201609064
中图分类号：收稿：2016-09-08，
纸质出版：2017-02-28
- 稿件说明：
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柏露, 周建荣, 王致娟, 等. 下调Cyclophilin A对喉鳞癌裸鼠移植瘤模型肿瘤生长和转移的影响[J]. 第三军医大学学报, 2017,39(4):355-360.
Lu Bai, Jianrong Zhou, Zhijuan Wang, et al. Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice[J]. J Third Mil Med Univ, 2017, 39(4): 355-360.
柏露, 周建荣, 王致娟, 等. 下调Cyclophilin A对喉鳞癌裸鼠移植瘤模型肿瘤生长和转移的影响[J]. 第三军医大学学报, 2017,39(4):355-360. DOI： 10.16016/j.1000-5404.201609064.
Lu Bai, Jianrong Zhou, Zhijuan Wang, et al. Effect of cyclophilin A downregulation on growth and metastasis of laryngeal squamous cell carcinoma in nude mice[J]. J Third Mil Med Univ, 2017, 39(4): 355-360. DOI： 10.16016/j.1000-5404.201609064.
摘要
Abstract
Objective
2
To explore the effect of RNA interference-mediated cyclophilin A (CypA) down-regulation on the growth and metastasis of laryngeal squamous cell carcinoma in nude mice.
Method
2
Lentiviral vector containing CypA shRNA was constructed and trans infected into Hep2 cells. Fifteen nude mice were randomly divided into 3 groups (
n
=5): control group
NC shRNA group
and CypA shRNA group. The growth and lymph node metastasis of tumors were observed. The expression of CypA
CD147 and matrix metalloproteinase (MMP)-9 in tumor tissues was detected by immunohistochemistry (IHC). The mRNA and protein expression levels of CypA
CD147 and MMP-9 were detected by real-time polymerase chain reaction (RT-PCR) assay and Western blotting
respectively.
Results
2
The gross tumor volume of CypA shRNA group was significantly smaller than those of both control and NC shRNA groups (
P
<
0.05). IHC staining showed cytoplasmic localization of CypA expression
whereas expression of CD147 and MMP-9 was detected in the cytomembrane and tumor stroma
respectively. The mRNA and protein expression levels of CypA
CD147 and MMP-9 were significantly decreased in CypA shRNA group as compared to control and NC shRNA groups (
P
<
0.05).
Conclusion
2
Down-regulation of cyclophilin A can inhibit the growth and metastasis of laryngeal squamous cell carcinoma by mediating the expression of CD147 and MMP-9.
关键词
Keywords
references
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