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晶状体蛋白αB对卵巢癌细胞恶性行为的影响
Effect of αB-crystallin on malignant behaviors of ovarian cancer cells
- 第三军医大学学报 2017年39卷第3期 页码：223-228
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(81572562)
DOI：10.16016/j.1000-5404.201609030
中图分类号：收稿：2016-09-04，
纸质出版：2017-02-15
- 稿件说明：
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张文君, 刘斌, 令狐华. 晶状体蛋白αB对卵巢癌细胞恶性行为的影响[J]. 第三军医大学学报, 2017,39(3):223-228.
Wenjun Zhang, Bin Liu, Hua Linghu. Effect of αB-crystallin on malignant behaviors of ovarian cancer cells[J]. Journal of Third Military Medical University, 2017, 39(3): 223-228.
张文君, 刘斌, 令狐华. 晶状体蛋白αB对卵巢癌细胞恶性行为的影响[J]. 第三军医大学学报, 2017,39(3):223-228. DOI： 10.16016/j.1000-5404.201609030.
Wenjun Zhang, Bin Liu, Hua Linghu. Effect of αB-crystallin on malignant behaviors of ovarian cancer cells[J]. Journal of Third Military Medical University, 2017, 39(3): 223-228. DOI： 10.16016/j.1000-5404.201609030.
摘要
Abstract
Objective
2
To explore the expression of αB-crystallin in epithelial ovarian cancer tissues and determine its effect on the proliferation
invasion and migration of human ovarian cancer SKOV3 cells.
Methods
2
The expression of αB-crystallin was detected by immunohistochemical assay in normal ovarian tissues
type Ⅰand type Ⅱ epithelial ovarian cancer tissues. SKOV3 cells
with high endogenous expression level of αB-crystallin
were transfected with lentiviral vector carrying the small interfering RNA (siRNA) targeted CRYAB gene (αB-crystallin gene). Apoptosis and cell proliferation were detected by flow cytometry and MTT assay as well as plate clone assay. Transwell chamber test and the matrigel invasion assay were performed to measure the migration and invasion respectively. The expression of matrix metalloproteinase 2 (MMP-2) and 9 (MMP-9) was measured by Western blotting.
Results
2
The expression of αB-crystallin was significantly higher in the epithelial ovarian cancer tissues than normal ovarian tissues
especially in the type Ⅱ epithelial ovarian cancer tissues (
P
<
0.01). Down-regulating αB-crystallin by lentiviral vector resulted in the decrease of αB-crystallin expression by 67%
but had no effect on the apoptosis and cell proliferation of the infected SKOV3 cells. However
αB-crystallin knock-down significantly inhibited the migration and invasion capacities (
P
<
0.01) as well as the expression levels of MMP-2 and MMP-9 in the SKOV3 cells (
P
<
0.01).
Conclusion
2
αB-crystallin affects invasion and migration of SKOV3 cells. Its high expression may be associated with higher metastatic potential of type Ⅱepithelial ovarian cancer.
关键词
Keywords
references
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