小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用

马剑波; 黎娟娟; 鲁开智; 顾健腾

doi:10.16016/j.1000-5404.201608179

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小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用

基础医学 | 更新时间：2026-01-09

- 小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用
- Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion
- 第三军医大学学报 2017年39卷第3期页码：249-252
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目(81272068)
- DOI：10.16016/j.1000-5404.201608179
  中图分类号：
- 收稿：2016-09-28，
  
  纸质出版：2017-02-15
- 稿件说明：
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马剑波, 黎娟娟, 鲁开智, 等. 小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用[J]. 第三军医大学学报, 2017,39(3):249-252.

Jianbo Ma, Juanjuan Li, Kaizhi Lu, et al. Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion[J]. Journal of Third Military Medical University, 2017, 39(3): 249-252.
马剑波, 黎娟娟, 鲁开智, 等. 小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用[J]. 第三军医大学学报, 2017,39(3):249-252. DOI： 10.16016/j.1000-5404.201608179.

Jianbo Ma, Juanjuan Li, Kaizhi Lu, et al. Recording of discharge of mouse jugular vagus and its application during renal ischemia-reperfusion[J]. Journal of Third Military Medical University, 2017, 39(3): 249-252. DOI： 10.16016/j.1000-5404.201608179.

摘要

Abstract

Objective

To record the frequency variations of jugular vagus discharge in mouse model of renal ischemia-reperfusion

and introduce the method and experimental skills of the recording.

Methods

The right cervical vagal nerve of 8 male C57BL/6J mice (8~10 weeks old

weighing 22~26 g) was exposed and then bluntly separated through

midline cervical incision. The vagus nerve discharge was conducted by

pair of bipolar stainless steel electrodes and then recorded. Renal ischemia was induced by bilateral renal pedicle clamping for 25 min and followed by reperfusion for 30 min. The frequency variations of vagus nerve discharge were recorded by the electrodes during the whole process.

Results

Compared with the basic values

the discharge frequency were increased from 520±52 Hz to 632±59 Hz (

0.05) in ischemic phase

and furthermore it kept in an increasing trend during 15 minutes' reperfusion and then reached the peak (672±58 Hz

0.05)

but then was gradually decreased to 544±51 Hz after reperfusion (

0.05).

Conclusion

The vagus discharge is significantly increased during renal ischemia-reperfusion

which might be due to neural protection in the process.

关键词

Keywords

references

Yap S C Lee H T Acute kidney injury and extrarenal organ dysfunction: new concepts and experimental evidence Anesthesiology 2012 116 5 1139 1148 10.1097/ALN.0b013e31824f951b Yap S C, Lee H T. Acute kidney injury and extrarenal organ dysfunction: new concepts and experimental evidence[J]. Anesthesiology, 2012, 116(5): 1139-1148. DOI: 10.1097/ALN.0b013e31824f951b

White L E Hassoun H T Inflammatory Mechanisms of Organ Crosstalk during Ischemic Acute Kidney Injury Int J Nephrol 2012 2012 505197 10.4061/2012/505197 White L E, Hassoun H T. Inflammatory Mechanisms of Organ Crosstalk during Ischemic Acute Kidney Injury[J]. Int J Nephrol, 2012, 2012: 505197. DOI: 10.4061/2012/505197

Gu J Chen J Xia P Dexmedetomidine attenuates remote lung injury induced by renal ischemia-reperfusion in mice Acta Anaesthesiol Scand 2011 55 10 1272 1278 10.1111/j.1399-6576.2011.02526.x Gu J, Chen J, Xia P, et al . Dexmedetomidine attenuates remote lung injury induced by renal ischemia-reperfusion in mice[J ] . Acta Anaesthesiol Scand, 2011, 55(10): 1272-1278. DOI: 10.1111/j.1399-6576.2011.02526.x

Borovikova L V Ivanova S Zhang M Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin Nature 2000 405 6785 458 462 10.1038/35013070 Borovikova L V, Ivanova S, Zhang M, et al . Vagus nerve stimulation attenuates the systemic inflammatory response to endotoxin[J ] . Nature, 2000, 405(6785): 458-462. DOI: 10.1038/35013070

Tracey K J The inflammatory reflex Nature 2002 420 6917 853 859 10.1038/nature01321 Tracey K J. The inflammatory reflex[J]. Nature, 2002, 420(6917): 853-859. DOI: 10.1038/nature01321

Xiang H Hu B Li Z Dexmedetomidine controls systemic cytokine levels through the cholinergic anti-inflammatory pathway Inflammation 2014 37 5 1763 1770 10.1007/s10753-014-9906-1 Xiang H, Hu B, Li Z, et al . Dexmedetomidine controls systemic cytokine levels through the cholinergic anti-inflammatory pathway[J ] . Inflammation, 2014, 37(5): 1763-1770. DOI: 10.1007/s10753-014-9906-1

Zhao M He X Bi X Y Vagal stimulation triggers peripheral vascular protection through the cholinergic anti-inflammatory pathway in a rat model of myocardial ischemia/reperfusion Basic Res Cardiol 2013 108 3 345 10.1007/s00395-013-0345-1 Zhao M, He X, Bi X Y, et al . Vagal stimulation triggers peripheral vascular protection through the cholinergic anti-inflammatory pathway in a rat model of myocardial ischemia/reperfusion[J ] . Basic Res Cardiol, 2013, 108(3): 345. DOI: 10.1007/s00395-013-0345-1

Liu D Zhang H G Chang M T Melanocortin-4 receptor agonists alleviate intestinal dysfunction in secondary intra-abdominal hypertension rat model J Surg Res 2015 195 1 263 270 10.1016/j.jss.2015.01.007 Liu D, Zhang H G, Chang M T, et al . Melanocortin-4 receptor agonists alleviate intestinal dysfunction in secondary intra-abdominal hypertension rat model[J ] . J Surg Res, 2015, 195(1): 263-270. DOI: 10.1016/j.jss.2015.01.007

Johnston G R Webster N R Cytokines and the immunomodulatory function of the vagus nerve Br J Anaesth 2009 102 4 453 462 10.1093/bja/aep037 Johnston G R, Webster N R. Cytokines and the immunomodulatory function of the vagus nerve[J]. Br J Anaesth, 2009, 102(4): 453-462. DOI: 10.1093/bja/aep037

Fernandez R Nardocci G Navarro C Neural reflex regulation of systemic inflammation: potential new targets for sepsis therapy Front Physiol 2014 5 489 10.3389/fphys.2014.00489 Fernandez R, Nardocci G, Navarro C, et al . Neural reflex regulation of systemic inflammation: potential new targets for sepsis therapy[J ] . Front Physiol, 2014, 5: 489. DOI: 10.3389/fphys.2014.00489

Kentish S J Vincent A D Kennaway D J High-Fat Diet-Induced Obesity Ablates Gastric Vagal Afferent Circadian Rhythms J Neurosci 2016 36 11 3199 3207 10.1523/JNEUROSCI.2710-15.2016 Kentish S J, Vincent A D, Kennaway D J, et al . High-Fat Diet-Induced Obesity Ablates Gastric Vagal Afferent Circadian Rhythms[J ] . J Neurosci, 2016, 36(11): 3199-3207. DOI: 10.1523/JNEUROSCI.2710-15.2016

Luyer M D de Haan J J Lubbers T Parasympathetic stimulation via the vagus nerve prevents systemic organ dysfunction by abrogating gut injury and lymph toxicity in trauma and hemorrhagic shock Shock 2013 39 5 460 461 10.1097/SHK.0b013e31828def5a Luyer M D, de Haan J J, Lubbers T, et al . Parasympathetic stimulation via the vagus nerve prevents systemic organ dysfunction by abrogating gut injury and lymph toxicity in trauma and hemorrhagic shock[J ] . Shock, 2013, 39(5): 460-461. DOI: 10.1097/SHK.0b013e31828def5a

Headland S E Norling L V The resolution of inflammation: Principles and challenges Semin Immunol 2015 27 3 149 160 10.1016/j.smim.2015.03.014 Headland S E, Norling L V. The resolution of inflammation: Principles and challenges[J]. Semin Immunol, 2015, 27(3): 149-160. DOI: 10.1016/j.smim.2015.03.014

Nathan C Points of control in inflammation Nature 2002 420 6917 846 852 10.1038/nature01320 Nathan C. Points of control in inflammation[J]. Nature, 2002, 420(6917): 846-852. DOI: 10.1038/nature01320

Atkinson S J A wandering path toward prevention for acute kidney injury J Clin Invest 2016 126 5 1640 1642 10.1172/JCI86826 Atkinson S J. A wandering path toward prevention for acute kidney injury[J]. J Clin Invest, 2016, 126(5): 1640-1642. DOI: 10.1172/JCI86826

Inoue T Abe C Sung S S Vagus nerve stimulation mediates protection from kidney ischemia-reperfusion injury through alpha7nAChR+ splenocytes J Clin Invest 2016 126 5 1939 1952 10.1172/JCI83658 Inoue T, Abe C, Sung S S, et al . Vagus nerve stimulation mediates protection from kidney ischemia-reperfusion injury through alpha7nAChR+ splenocytes[J ] . J Clin Invest, 2016, 126(5): 1939-1952. DOI: 10.1172/JCI83658

Lee S W Li Q Libbus I Chronic cyclic vagus nerve stimulation has beneficial electrophysiological effects on healthy hearts in the absence of autonomic imbalance Physiol Rep 2016 4 9 e12786 10.14814/phy2.12786 Lee S W, Li Q, Libbus I, et al . Chronic cyclic vagus nerve stimulation has beneficial electrophysiological effects on healthy hearts in the absence of autonomic imbalance[J ] . Physiol Rep, 2016, 4(9): e12786. DOI: 10.14814/phy2.12786

Bewernick B Schlaepfer T E Update on Neuromodulation for Treatment-Resistant Depression F1000Res 2015 4 F1000 Faculty Rev 1389 10.12688/f1000research.6633.1 Bewernick B, Schlaepfer T E. Update on Neuromodulation for Treatment-Resistant Depression[J]. F1000Res, 2015, 4 (F1000 Faculty Rev): 1389. DOI: 10.12688/f1000research.6633.1

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