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缺血再灌注大鼠肾组织及尿液DcR2表达变化与肾损伤的关系
DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats
- 第三军医大学学报 2017年39卷第4期 页码：342-348
- 作者机构：
- 作者简介：
- 基金信息：国家自然科学基金面上项目(8164792)
DOI：10.16016/j.1000-5404.201608163
中图分类号：收稿：2016-08-30，
纸质出版：2017-02-28
- 稿件说明：
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刘佳睿, 陈佳, 陈客宏, 等. 缺血再灌注大鼠肾组织及尿液DcR2表达变化与肾损伤的关系[J]. 第三军医大学学报, 2017,39(4):342-348.
Jiarui Liu, Jia Chen, Kehong Chen, et al. DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats[J]. J Third Mil Med Univ, 2017, 39(4): 342-348.
刘佳睿, 陈佳, 陈客宏, 等. 缺血再灌注大鼠肾组织及尿液DcR2表达变化与肾损伤的关系[J]. 第三军医大学学报, 2017,39(4):342-348. DOI： 10.16016/j.1000-5404.201608163.
Jiarui Liu, Jia Chen, Kehong Chen, et al. DcR2 expression in renal tissue and urine after ischemia reperfusion related to kidney injury in rats[J]. J Third Mil Med Univ, 2017, 39(4): 342-348. DOI： 10.16016/j.1000-5404.201608163.
摘要
Abstract
Objective
2
To observe the expression of decoy receptor 2 (DcR2) in renal tissue and urine of rat after ischemia reperfusion injury (I/R) for investigating its correlation to kidney injury.
Method
2
Forty healthy male SD rats were randomized into sham operation group and I/R 60 min group. Blood
urine and bilateral renal tissues were collected at days 1
3
7
21 and 35 after operation. The levels of serum creatinine (Scr) and urinary NAG (uNAG) were detected by biochemical analysis. Renal morphologic changes were observed and pathologically assessed by PAS and Masson's trichrome staining. The expression of DcR2 in renal tissues was detected by immunohistochemical assay
and the expression of urine DcR2 (uDcR2) was measured by enzyme-linked immunosorbent assay (ELISA).
Results
2
Compared to the Scr and uNAG levels in the sham group (21.3±1.6 and 4.4±0.6 U/mmol
respectively)
they significantly increased in the I/R 60 min group(
P
<
0.05). At days 1
3
7
21 and 35 post operation
the Scr levels were 122.2±15.4
193.5±27.7
66.4±10.2
37.8±6.2
and 34.4±2.8 μmol/L
respectively. Accordingly
the uNAG levels were 9.8±2.0
17.0±2.5
7.8±1.5
6.5±2.1
6.7±2.3 U/mmol
respectively. Obvious pathological changes were found at the corticomedullary junction in the I/R 60 min group
including acute tubular necrosis
denudation
brush border and formation of cellular casts
which progressed to renal tubule atrophy
interstitial fibrosis and chronic kidney disease in later stage of reperfusion. Additionally
the expression of DcR2 in renal tissue and urine was increased significantly in the I/R 60 min group compared to the sham group(
P
<
0.05). The increased level of DcR2 and uDcR2 were positively correlated with renal function and kidney injury score (
P
<
0.05). Meanwhile
the Masson staining showed that in the I/R 60 min group renal DcR2 expression was positively correlated with interstitial fibrosis (
P
<
0.05).
Conclusion
2
The expression of DcR2 in renal tissue and uDcR2 level are positively related to renal function and kidney injury score in rats with I/R injury. This may suggest the important role of DcR2 played in the progress of renal interstitial fibrosis after ischemia reperfusion.
关键词
Keywords
references
Bellomo R Decade in review--acute kidney injury: Acute kidney injury--a decade of progress Nat Rev Nephrol 2015 11 11 636 637 10.1038/nrneph.2015.147 Bellomo R. Decade in review--acute kidney injury: Acute kidney injury--a decade of progress[J]. Nat Rev Nephrol, 2015,11(11): 636-637.DOI: 10.1038/nrneph.2015.147
Xu X Nie S Liu Z Epidemiology and Clinical Correlates of AKI in Chinese Hospitalized Adults Clin J Am SocNephrol 2015 10 9 1510 1518 10.2215/CJN.02140215 Xu X, Nie S, Liu Z, et al. Epidemiology and Clinical Correlates of AKI in Chinese Hospitalized Adults[J]. Clin J Am SocNephrol, 2015, 10(9): 1510-1518.DOI: 10.2215/CJN.02140215
Bouchard J Acharya A Cerda J A Prospective International Multicenter Study of AKI in the Intensive Care Unit Clin J Am Soc Nephrol 2015 10 8 1324 1331 10.2215/CJN.04360514 Bouchard J, Acharya A, Cerda J, et al. A Prospective International Multicenter Study of AKI in the Intensive Care Unit[J].Clin J Am Soc Nephrol, 2015, 10(8): 1324-1331.DOI: 10.2215/CJN.04360514
Takaori K Yanagita M Insights into the Mechanisms of the Acute Kidney Injury-to-Chronic Kidney Disease Continuum Nephron 2016 134 3 172 176 10.1159/000448081 Takaori K, Yanagita M. Insights into the Mechanisms of the Acute Kidney Injury-to-Chronic Kidney Disease Continuum[J]. Nephron, 2016, 134(3): 172-176.DOI: 10.1159/000448081
Coca S G Singanamala S Parikh C R Chronic kidney disease after acute kidney injury: a systematic review and meta-analysis Kidney Int 2012 81 5 442 448 10.1038/ki.2011.379 Coca S G, Singanamala S, Parikh C R. Chronic kidney disease after acute kidney injury: a systematic review and meta-analysis[J]. Kidney Int, 2012, 81(5): 442-448.DOI: 10.1038/ki.2011.379
Carruthers K H Metzger G Choi E A Therapeutic Role for Survivin in Mitigating the Harmful Effects of Ionizing Radiation Sarcoma 2016 2016 6 1 8 10.1155/2016/1830849 Carruthers K H, Metzger G, Choi E, et al. A Therapeutic Role for Survivin in Mitigating the Harmful Effects of Ionizing Radiation[J]. Sarcoma, 2016, 2016(6):1-8.DOI: 10.1155/2016/1830849
霍 本刚 戴 欢子 张 建国 IgA 肾病肾组织诱骗受体 2 表达变化与肾小管、间质损伤的相关性分析 临床内科杂志 2014 31 5 344 347 霍本刚, 戴欢子, 张建国, 等. IgA 肾病肾组织诱骗受体 2 表达变化与肾小管、间质损伤的相关性分析[J]. 临床内科杂志, 2014, 31(5): 344-347.
Wan X Hou L J Zhang L Y IKKalpha is involved in kidney recovery and regeneration of acute ischemia/reperfusion injury in mice through IL10-producing regulatory T cells Dis Model Mech 2015 8 7 733 742 10.1242/dmm.018200 Wan X, Hou L J, Zhang L Y, et al. IKKalpha is involved in kidney recovery and regeneration of acute ischemia/reperfusion injury in mice through IL10-producing regulatory T cells[J]. Dis Model Mech, 2015, 8(7): 733-742.DOI: 10.1242/dmm.018200
Cheng H Fan X Lawson W E Telomerase deficiency delays renal recovery in mice after ischemia-reperfusion injury by impairing autophagy Kidney Int 2015 88 1 85 94 10.1038/ki.2015.69 Cheng H, Fan X, Lawson W E, et al. Telomerase deficiency delays renal recovery in mice after ischemia-reperfusion injury by impairing autophagy[J]. Kidney Int, 2015, 88(1): 85-94.DOI: 10.1038/ki.2015.69
Yang L Besschetnova T Y Brooks C R Epithelial cell cycle arrest in G2/M mediates kidney fibrosis after injury Nat Med 2010 16 5 535 543 10.1038/nm.2144 Yang L, Besschetnova T Y, Brooks C R, et al. Epithelial cell cycle arrest in G2/M mediates kidney fibrosis after injury[J].Nat Med, 2010, 16(5): 535-543, 1p-143p.DOI: 10.1038/nm.2144
Feng L Xiong Y Cheng F Effect of ligustrazine on ischemia-reperfusion injury in murine kidney Transplant Proc 2004 36 7 1949 1951 10.1016/j.transproceed.2004.07.050 Feng L, Xiong Y, Cheng F, et al. Effect of ligustrazine on ischemia-reperfusion injury in murine kidney[J]. Transplant Proc, 2004, 36(7): 1949-1951.DOI: 10.1016/j.transproceed.2004.07.050
Melk A Baisantry A Schmitt R The yin and yang of autophagy in acute kidney injury Autophagy 2016 12 3 596 597 10.1080/15548627.2015.1135284 Melk A, Baisantry A, Schmitt R. The yin and yang of autophagy in acute kidney injury[J]. Autophagy, 2016, 12(3):596-597.DOI: 10.1080/15548627.2015.1135284
Ferenbach D A Bonventre J V Mechanisms of maladaptive repair after AKI leading to accelerated kidney ageing and CKD Nat Rev Nephrol 2015 11 5 264 276 10.1038/nrneph.2015.3 Ferenbach D A, Bonventre J V. Mechanisms of maladaptive repair after AKI leading to accelerated kidney ageing and CKD[J]. Nat Rev Nephrol, 2015, 11(5): 264-276.DOI: 10.1038/nrneph.2015.3
Bonventre J V Yang L Cellular pathophysiology of ischemic acute kidney injury J Clin Invest 2011 121 11 4210 4221 10.1172/JCI45161 Bonventre J V, Yang L. Cellular pathophysiology of ischemic acute kidney injury[J]. J Clin Invest, 2011, 121(11):4210-4221.DOI: 10.1172/JCI45161
Kusaba T Lalli M Kramann R Differentiated kidney epithelial cells repair injured proximal tubule Proc Natl Acad Sci U S A 2014 111 4 1527 1532 10.1073/pnas.1310653110 Kusaba T, Lalli M, Kramann R, et al. Differentiated kidney epithelial cells repair injured proximal tubule[J]. Proc Natl Acad Sci U S A, 2014, 111(4): 1527-1532.DOI: 10.1073/pnas.1310653110
Portilla D Apoptosis, fibrosis and senescence Nephron Clin Pract 2014 127 1-4 65 69 10.1159/000363717 Portilla D. Apoptosis, fibrosis and senescence[J]. Nephron Clin Pract, 2014, 127(1-4): 65-69.DOI: 10.1159/000363717
Duiker E W Mom C H de Jong S The clinical trail of TRAIL Eur J Cancer 2006 42 14 2233 2240 10.1016/j.ejca.2006.03.018 Duiker E W, Mom C H, de Jong S, et al. The clinical trail of TRAIL[J]. Eur J Cancer, 2006, 42(14): 2233-2240.DOI: 10.1016/j.ejca.2006.03.018
Munoz-Espin D Serrano M Cellular senescence: from physiology to pathology Nat Rev Mol Cell Biol 2014 15 7 482 496 10.1038/nrm3823 Munoz-Espin D, Serrano M. Cellular senescence: from physiology to pathology[J]. Nat Rev Mol Cell Biol, 2014, 15(7):482-496. DOI: 10.1038/nrm3823
Tchkonia T Zhu Y van Deursen J Cellular senescence and the senescent secretory phenotype: therapeutic opportunities J Clin Invest 2013 123 3 966 972 10.1038/nrm3823 Tchkonia T, Zhu Y, van Deursen J, et al. Cellular senescence and the senescent secretory phenotype: therapeutic opportunities[J]. J Clin Invest, 2013, 123(3): 966-972.DOI: 10.1038/nrm3823
Denic A Glassock R J Rule A D Structural and Functional Changes With the Aging Kidney Adv Chronic Kidney Dis 2016 23 1 19 28 10.1053/j.ackd.2015.08.004 Denic A, Glassock R J, Rule A D. Structural and Functional Changes With the Aging Kidney[J]. Adv Chronic Kidney Dis,2016, 23(1): 19-28.DOI: 10.1053/j.ackd.2015.08.004
Sagiv A Biran A Yon M Granule exocytosis mediates immune surveillance of senescent cells Oncogene 2013 32 15 1971 1977 10.1038/onc.2012.206 Sagiv A, Biran A, Yon M, et al. Granule exocytosis mediates immune surveillance of senescent cells[J]. Oncogene, 2013,32(15): 1971-1977.DOI: 10.1038/onc.2012.206
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