SD大鼠房颤模型的构建及其外周血IL-17A变化的实验研究

李阅历; 钟理; 杨攀; 陈兴华; 邬娜; 李亚斐

doi:10.16016/j.1000-5404.201608162

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SD大鼠房颤模型的构建及其外周血IL-17A变化的实验研究

基础医学 | 更新时间：2026-01-09

- SD大鼠房颤模型的构建及其外周血IL-17A变化的实验研究
- Establishment of SD rat model for atrial fibrillation and changes of circulating IL-17A level
- 第三军医大学学报 2017年39卷第3期页码：253-258
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201608162
  中图分类号：
- 收稿：2016-08-29，
  
  纸质出版：2017-02-15
- 稿件说明：
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李阅历, 钟理, 杨攀, 等. SD大鼠房颤模型的构建及其外周血IL-17A变化的实验研究[J]. 第三军医大学学报, 2017,39(3):253-258.

Yueli Li, Li Zhong, Pan Yang, et al. Establishment of SD rat model for atrial fibrillation and changes of circulating IL-17A level[J]. Journal of Third Military Medical University, 2017, 39(3): 253-258.
李阅历, 钟理, 杨攀, 等. SD大鼠房颤模型的构建及其外周血IL-17A变化的实验研究[J]. 第三军医大学学报, 2017,39(3):253-258. DOI： 10.16016/j.1000-5404.201608162.

Yueli Li, Li Zhong, Pan Yang, et al. Establishment of SD rat model for atrial fibrillation and changes of circulating IL-17A level[J]. Journal of Third Military Medical University, 2017, 39(3): 253-258. DOI： 10.16016/j.1000-5404.201608162.

摘要

Abstract

Objective

To establish an animal model of atrial fibrillation (AF) which is similar to clinical pathogenesis and pathological changes in a short period

and to investigate the changes of circulating interleukin-17A (IL-17A) and C reactive protein (CRP) levels.

Methods

Twenty rats were randomly divided into AF group and negative control group. Transesophagael atrial rapid pacing was conducted in the AF group. The induction of AF was recorded and electrocardiography (ECG) was carried out. The concentrations of IL-17A and CRP in the plasma were measured by enzyme-linked immunosorbent assay (ELISA) and the atrial tissue fibrosis was evaluated by Masson trichrome staining.

Results

AF was induced in all rats of the AF group after atrial rapid pacing. The total inducibility was 96% and the average duration was 45.52±60.88 s. Compared to the negative control group

the atrial rate

QRS interval and QT interval were extended significantly in AF group (

0.05). The concentrations of IL-17A and CRP were significantly higher in the AF group than the control group

and were increased with the increased number of AF occurrence (

0.05). The fibrosis of atrial tissue was more serious in the AF group with higher collagen volume fraction (

0.05).

Conclusion

The induction of AF in SD rats by rapid transesophageal atrial pacing is an effective way with high successful rate

good repeatability and short period

which was one of the ideal ways to induce AF. IL-17A and CRP might play roles in the occurrence and development of AF.

关键词

Keywords

references

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