海马细胞胞浆RARα表达对大鼠学习记忆功能的影响

吴小凤; 任兰; 侯娜丽; 杨亭; 何慕兰; 谷燕; 黄鸿眉; 陈洁; 李廷玉

doi:10.16016/j.1000-5404.201608025

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海马细胞胞浆RARα表达对大鼠学习记忆功能的影响

基础医学 | 更新时间：2026-01-09

- 海马细胞胞浆RARα表达对大鼠学习记忆功能的影响
- Effect of cytoplasmic localization of retinoic acid receptor α in hippocampal cells on learning-memory function in rats
- 第三军医大学学报 2017年39卷第3期页码：208-215
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金-中加合作重点项目(81161120498);国家自然科学基金面上项目(81271385);国家自然科学基金面上项目(81100476)
- DOI：10.16016/j.1000-5404.201608025
  中图分类号：
- 收稿：2016-08-04，
  
  纸质出版：2017-02-15
- 稿件说明：
移动端阅览
吴小凤, 任兰, 侯娜丽, 等. 海马细胞胞浆RARα表达对大鼠学习记忆功能的影响[J]. 第三军医大学学报, 2017,39(3):208-215.

Xiaofeng Wu, Lan Ren, Nali Hou, et al. Effect of cytoplasmic localization of retinoic acid receptor α in hippocampal cells on learning-memory function in rats[J]. Journal of Third Military Medical University, 2017, 39(3): 208-215.
吴小凤, 任兰, 侯娜丽, 等. 海马细胞胞浆RARα表达对大鼠学习记忆功能的影响[J]. 第三军医大学学报, 2017,39(3):208-215. DOI： 10.16016/j.1000-5404.201608025.

Xiaofeng Wu, Lan Ren, Nali Hou, et al. Effect of cytoplasmic localization of retinoic acid receptor α in hippocampal cells on learning-memory function in rats[J]. Journal of Third Military Medical University, 2017, 39(3): 208-215. DOI： 10.16016/j.1000-5404.201608025.

摘要

Abstract

Objective

To determine the effect of the cytoplasm retinoic acid receptor α (RARα) expressed in hippocampal cells on learning-memory function in rats.

Methods

Sprague-Dawley rats were randomly divided into the Morris water maze (MWM) trained group (

=6) and the untrained group (

=6). The expression levels of RARα and GluR1 in the hippocampus of the 2 groups were measured after training by PCR and Western blotting. After the RARα shRNAs adeno-associated virus (AAV) and AAV-control shRNA were respectively injected stereotactically into the hippocampus of rats

the learning-memory function was tested by MWM test and the expression levels of RARα and GluR1 in the hippocampus were measured by PCR and Western blotting. After the PC12 cells induced by neuron growth factor (NGF)

in vitro

for 7 d to differentiate into neuron-like cells

the expression levels of RARα and GluR1

the calcium activity and the resting membrane potential level were detected.

Results

MWM training enhanced the learning-memory function

and the total protein and cytoplasm protein levels of RARα and GluR1 in the hippocampus when compared with the untrained rats (

0.01). After the intra-hippocampal injection of AAV-RARα shRNAs for 2 weeks

MWM test results showed that their learning-memory function was statistically decreased than the AAV-control shRNA rats (

0.05). Meanwhile

the total protein and cytoplasm protein levels of RARα and

GluR1 in the hippocampus were markedly decreased in the AAV-RARα shRNAs injected rats (

0.01). As a neuron model

in vitro

the resting membrane potential level of PC12 cells induced by NGF treatment was much closer to that of the neurons (

0.01)

the total and cytoplasm levels of RARα and GluR1 were also obviously increased compared with those of the control cells (

0.01)

and the cellular calcium activity was enhanced (

0.05).

Conclusion

The cytoplasmic expression level of RARα in the hippocampal cells is positively correlated with the learning-memory ability

and play an important role in regulating synaptic plasticity.

关键词

Keywords

references

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