登革病毒感染人原代树突状细胞（CD209,-139A/-336A）模型的建立

杨裔; 方昕; 胡珍; 杨杰; 袁吉振; 尚伟龙; 饶贤才

doi:10.16016/j.1000-5404.201607134

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登革病毒感染人原代树突状细胞（CD209,-139A/-336A）模型的建立

军事医学：热带医学 | 更新时间：2026-01-09

- 登革病毒感染人原代树突状细胞（CD209,-139A/-336A）模型的建立
- Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus
- 第三军医大学学报 2017年39卷第4期页码：323-327
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重大科技专项(2012ZX09103301-038);国家自然科学基金青年科学基金(31200133)
- DOI：10.16016/j.1000-5404.201607134
  中图分类号：
- 收稿：2016-07-20，
  
  纸质出版：2017-02-28
- 稿件说明：
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杨裔, 方昕, 胡珍, 等. 登革病毒感染人原代树突状细胞（CD209,-139A/-336A）模型的建立[J]. 第三军医大学学报, 2017,39(4):323-327.

Yi Yang, Xin Fang, Zhen Hu, et al. Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus[J]. J Third Mil Med Univ, 2017, 39(4): 323-327.
杨裔, 方昕, 胡珍, 等. 登革病毒感染人原代树突状细胞（CD209,-139A/-336A）模型的建立[J]. 第三军医大学学报, 2017,39(4):323-327. DOI： 10.16016/j.1000-5404.201607134.

Yi Yang, Xin Fang, Zhen Hu, et al. Establishment of a human primary dendritic cell model (CD209,-139A/-336A)infected with dengue virus[J]. J Third Mil Med Univ, 2017, 39(4): 323-327. DOI： 10.16016/j.1000-5404.201607134.

摘要

Abstract

Objective

To establish a human primary dendritic cells(DCs) model infected with dengue virus (DENV).

Method

Peripheral blood samples defined with DC genotype of CD209

-139A/-336A were collected. The DC precursor cells were isolated by ficoll-gastrografin density gradient centrifugation and further induced to differentiate into immature DCs by culturing in RPMI1640 media supplemented with recombinant human granulocyte macrophage-colony stimulating factor (rhGM-CSF) and interleukin-4 (rhIL-4). The differentiation efficiency was determined by flow cytometry.The morphologic and biological characteristics of DCs were identified by Wright’s staining and lymphocyte proliferation assay

respectively. Finally

DENV-1 strain GZ2002 was used for construction and evaluation of the DENV infection in human DCs.

Results

The immature DCs with typical morphologic characteristics were successfully obtained from the isolated precursor cells with cytokine stimulation

of which the differentiation efficiency was greater than 90%. The acquired DCs could effectively stimulate lymphocyte proliferation and support the entire viral lifecycle during DENV infection

suggesting the successful establishment of DENV infected DC model.

Conclusion

DENV infection model based on human primary DCs is successfully established.

关键词

Keywords

references

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