Ⅰ型登革病毒感染后不同病程病毒核酸及其IgM抗体的变化规律

张培; 李春缘; 陈迪嘉; 李曦; 于德宪; 谢晓波; 陈月; 严华成; 任瑞文

doi:10.16016/j.1000-5404.201606170

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Ⅰ型登革病毒感染后不同病程病毒核酸及其IgM抗体的变化规律

军事医学：热带医学 | 更新时间：2026-01-09

- Ⅰ型登革病毒感染后不同病程病毒核酸及其IgM抗体的变化规律
- Dynamics of viral RNA and IgM antibody in patients with dengue virus type 1 infection
- 第三军医大学学报 2017年39卷第4期页码：328-332
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省科技计划项目(2014A020219007，2016A020219006，2014A020212252，2015A020218003);军队科技计划项目(CGZ14J006);广东省医学科学技术研究基金(A2015571)
- DOI：10.16016/j.1000-5404.201606170
  中图分类号：
- 收稿：2016-06-29，
  
  纸质出版：2017-02-28
- 稿件说明：
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张培, 李春缘, 陈迪嘉, 等. Ⅰ型登革病毒感染后不同病程病毒核酸及其IgM抗体的变化规律[J]. 第三军医大学学报, 2017,39(4):328-332.

Pei Zhang, Chunyuan Li, Dijia Chen, et al. Dynamics of viral RNA and IgM antibody in patients with dengue virus type 1 infection[J]. J Third Mil Med Univ, 2017, 39(4): 328-332.
张培, 李春缘, 陈迪嘉, 等. Ⅰ型登革病毒感染后不同病程病毒核酸及其IgM抗体的变化规律[J]. 第三军医大学学报, 2017,39(4):328-332. DOI： 10.16016/j.1000-5404.201606170.

Pei Zhang, Chunyuan Li, Dijia Chen, et al. Dynamics of viral RNA and IgM antibody in patients with dengue virus type 1 infection[J]. J Third Mil Med Univ, 2017, 39(4): 328-332. DOI： 10.16016/j.1000-5404.201606170.

摘要

Abstract

Objective

To investigate the dynamics of viral RNA and IgM antibody in patients infected with dengue virus type 1 (DV1)

in order to provide theoretical basis of rapid and accurate diagnosis of dengue fever.

Method

Clinical data and serum samples of DV1 infected patients from July to November 2014 were collected and retrospectively analyzed in this study. The dynamics of viral RNA and IgM antibody during the course of disease were analyzed by quantitative real-time reverse transcriptase polymerase chain reaction(RT-PCR) and enzyme-linked immunosorbent assay (ELISA).

Results

A total of 222 positive samples including 137 positive for viral RNA

146 positive for IgM

and 61 positive for both were detected. The positive ratio of viral RNA was about 75%~100% in acute phase (day 1 to 5). While the positive ratio of IgM antibody was low on day 1 to 2

but it increased quickly to 50%~70% on day 3 to 5. In convalescent phase (day 6 to 10)

the positive ratio of viral RNA rapidly decreased and was only about 20% after day 8. Meanwhile

the positive ratio of IgM antibody increased to 95%~100%. There was a statistically significant difference in the constituent ratio of viral RNA and IgM antibody

i.e.

DV1(+) IgM(-)

DV1(+) IgM(+)

DV1(-) IgM(+)

between the acute phase and convalescent phase (

=63.41

0.05).

Conclusion

In patients infected with DV1

the positive ratio of viral RNA is above 75% in acute phase

while the positive ratio of IgM antibody is above 95% in convalescent phase. The efficiency of diagnosis could be improved by applying more reasonable and effective detection assays according to the course of disease.

关键词

Keywords

references

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