骨形成蛋白和激活素的跨膜抑制剂调节自噬抑制谷氨酸诱导的大鼠脊髓背角神经组织细胞凋亡

张志鑫; 崔应麟; 李彦杰; 任锟; 邢若星

doi:10.16016/j.1000-5404.201606009

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骨形成蛋白和激活素的跨膜抑制剂调节自噬抑制谷氨酸诱导的大鼠脊髓背角神经组织细胞凋亡

基础医学 | 更新时间：2026-01-09

- 骨形成蛋白和激活素的跨膜抑制剂调节自噬抑制谷氨酸诱导的大鼠脊髓背角神经组织细胞凋亡
- BAMBI inhibits apoptosis in glutamate induced neurons and astrocytes in spinal dorsal horn of rats via regulating autophagy
- 第三军医大学学报 2017年39卷第3期页码：259-264
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201606009
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- 收稿：2016-06-01，
  
  纸质出版：2017-02-15
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张志鑫, 崔应麟, 李彦杰, 等. 骨形成蛋白和激活素的跨膜抑制剂调节自噬抑制谷氨酸诱导的大鼠脊髓背角神经组织细胞凋亡[J]. 第三军医大学学报, 2017,39(3):259-264.

Zhixin Zhang, Yinglin Cui, Yanjie Li, et al. BAMBI inhibits apoptosis in glutamate induced neurons and astrocytes in spinal dorsal horn of rats via regulating autophagy[J]. Journal of Third Military Medical University, 2017, 39(3): 259-264.
张志鑫, 崔应麟, 李彦杰, 等. 骨形成蛋白和激活素的跨膜抑制剂调节自噬抑制谷氨酸诱导的大鼠脊髓背角神经组织细胞凋亡[J]. 第三军医大学学报, 2017,39(3):259-264. DOI： 10.16016/j.1000-5404.201606009.

Zhixin Zhang, Yinglin Cui, Yanjie Li, et al. BAMBI inhibits apoptosis in glutamate induced neurons and astrocytes in spinal dorsal horn of rats via regulating autophagy[J]. Journal of Third Military Medical University, 2017, 39(3): 259-264. DOI： 10.16016/j.1000-5404.201606009.

摘要

Abstract

Objective

To determine the effect of the over-expression of BMP and activin membrane-bound inhibitor (BAMBI) on the apoptosis of rat neurons and astrocytes in spinal dorsal horn induced by glutamate (Glu).

Methods

Neurons and astrocytes were isolated from spinal dorsal horn of newborn Wistar rats

and primarily cultured. Then the cells were randomly divided into 6 groups: control group

Glu group

Glu+pcDNA group

Glu+pcDNA-BAMBI group

Glu+siRNA control group and Glu+BAMBI siRNA group. The expression of BAMBI was measured by Western blotting. Cell apoptosis was detected by flow cytometry. The expression of microtubule associated protein light chain 3 (LC3)

Beclin1 and p62 was measured by Western blotting. The content of GFP-LC3 in the cultured cell groups was observed by fluorescence microscopy. Finally

the levels of tumor necrosis factor-α (TNF-α)

interleukin-1β (IL-1β) and cleaved-Caspase3 were detected by Western blot analysis.

Results

Compared to the control cells

Glu treatment induced cell apoptosis

decreased the expression of LC3-Ⅰ

promoted the expression levels of LC3-Ⅱ

Beclin1 and p62

increased the content of GFP-LC3

and elevated the level of TNF-α

IL-1β and cleaved-Caspase 3 (

0.05). However

when compared with Glu treatment group

BAMBI overexpression inhibited the cell apoptosis

strongly promoted the expression of LC3 and Beclin1

while decreased the levels of LC3-Ⅰ and p62

significantly declined the levels of TNF-α

IL-1β and cleaved-Caspase3 (

0.05)

and induced a large accumulation of GFP-LC3 in the cells. The effects of BAMBI silence group was opposite to those of the overexpression.

Conclusion

The overexpression of BAMBI could enhance autophagy and inhibit apoptosis in glutamate-induced neurons and astrocytes in rat spinal dorsal horn.

关键词

Keywords

references

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